Anti-PAX6 antibody [AD2.38]

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-PAX6 antibody [AD2.38] (AB78545)

Immunohistochemical analysis of 10% paraformaldehyde-fixed frozen Human pancreas tissue labeling PAX6 with ab78545 at 1/1000 dilution.

Nuclear staining in pancreas islets. IHC-fr image of PAX6 staining in a section of frozen normal human pancreas performed on a Leica Biosystems BOND^® RX instrument. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab78545, 1ug/ml dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [AD2.38] (AB78545)

Immunohistochemical analysis of formalin fixed paraffin embedded Human pancreas tissue labelling PAX6 with ab78545 at a concentration of 1/500 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Nuclear staining on human pancreas. The section was incubated with ab78545 for 30 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Sections were counterstained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [AD2.38] (AB78545)

Immunohistochemical analysis of formalin fixed paraffin embedded Human retina tissue labelling PAX6 with ab78545 at a concentration of 1/500 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Nuclear staining on human retina. The section was incubated with ab78545 for 30 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Sections were counterstained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [AD2.38] (AB78545)

Immunohistochemical analysis of formalin fixed paraffin embedded Rat pancreas tissue labelling PAX6 with ab78545 at a concentration of 1/500 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Nuclear staining on rat pancreas. The section was incubated with ab78545 for 30 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Sections were counterstained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [AD2.38] (AB78545)

Immunohistochemical analysis of formalin fixed paraffin embedded Mouse pancreas tissue labelling PAX6 with ab78545 at a concentration of 1/500 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Nuclear staining on mouse pancreas. The section was incubated with ab78545 for 30 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Sections were counterstained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [AD2.38] (AB78545)

Immunohistochemical analysis of formalin fixed paraffin embedded Rat retina tissue labelling PAX6 with ab78545 at a concentration of 1/500 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Nuclear staining on rat retina. The section was incubated with ab78545 for 30 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Sections were counterstained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [AD2.38] (AB78545)

Immunohistochemical analysis of formalin fixed paraffin embedded Mouse retina tissue labelling PAX6 with ab78545 at a concentration of 1/500 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Nuclear staining on mouse retina. The section was incubated with ab78545 for 30 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Sections were counterstained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-PAX6 antibody [AD2.38] (AB78545)

Immunohistochemical analysis of 10% paraformaldehyde-fixed frozen Mouse pancreas tissue labeling PAX6 with ab78545 at 1/1000 dilution.

Nuclear staining in pancreas islets. IHC-fr image of PAX6 staining in a section of frozen normal mouse pancreas performed on a Leica Biosystems BOND^® RX instrument. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab78545, 1ug/ml dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-PAX6 antibody [AD2.38] (AB78545)

Immunohistochemical analysis of 10% paraformaldehyde-fixed frozen Rat pancreas tissue labeling PAX6 with ab78545 at 1/1000 dilution.

Nuclear staining in pancreas islets. IHC-fr image of PAX6 staining in a section of frozen normal mouse pancreas performed on a Leica Biosystems BOND^® RX instrument. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab78545, 1ug/ml dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [AD2.38] (AB78545)

Immunohistochemical analysis of formalin fixed paraffin embedded Mouse skeletal muscle tissue labelling PAX6 with ab78545 at a concentration of 1/500 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on the mouse skeletal muscle. The section was incubated with ab78545 for 30 mins at room temperature, followed by anti-Mouse IgG1 antibody (ab125913) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Sections were counterstained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-PAX6 antibody [AD2.38] (AB78545)

Immunofluorescent analysis of 4% Paraformaldehyde fixed, 0.1% Triton X-100 permeabilized Primary mouse neurons/glia, DIV1 and DIV14 with ab78545 at 1/200 dilution followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 dilution (Green). ab7291 Anti-alpha Tubulin antibody was used for counterstain at a 1/1000 dilution followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) preadsorbed at 1/1000 dilution (Red). Nuclear DNA was labelled with DAPI (shown in blue).

ab78545 staining Pax6 in primary mouse neurons/glia, DIV1 (top row) and DIV14 (bottom row) both prepared from E18 mouse hippocampal brain area (obtained from Transnetyx Tissue by BrainBits, LLC, cat.no. C57EHP). A subset of cells show staining in the nucleus at DIV1 (likely neuroprogenitor cells), while differentiated neurons (DIV14) are largely Pax6 negative.

• IHC-Fr

AbReview32699****

Immunohistochemistry (Frozen sections) - Anti-PAX6 antibody [AD2.38] (AB78545)

Immunohistochemical analysis of mouse brain tissue, staining PAX6 with ab78545.

Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/100 in PBST) for 12 hours at 4°C. An AlexaFluor®568-conjugated goat anti-mouse polyclonal IgG (1/1000) was used as the secondary antibody.
This image was generated using a previous batch manufactured using hybridoma production method.

This image is courtesy of an anonymous Abreview.

• IHC-Fr

AbReview51262****

Immunohistochemistry (Frozen sections) - Anti-PAX6 antibody [AD2.38] (AB78545)

Immunohistochemical analysis of PFA-fixed frozen murine embryonic brain coronal sections, labelling PAX6 with ab78545 at a dilution of 1/100 incubated for 8 hours at 4°C in blocking buffer diluent. Permeabilization was with Triton X-100 and blocking was with 1% serum for 1 hour. Heat mediated antigen retrival was with 10mM sodium citrate buffer pH6.0 for 10 minutes at 650W in a microwave. The secondary was a goat Alexa Fluor^® 488 at 1/700.
This image was generated using a previous batch manufactured using hybridoma production method

Image is courtesy of an AbReview submitted by Dr Bhavin Shah.