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AB212694

Anti-PAX6 antibody [PAX6/498] - BSA and Azide free

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(1 Publication)

Mouse Monoclonal PAX6 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PAX6 aa 1-300.

View Alternative Names

AN2, PAX6, Paired box protein Pax-6, Aniridia type II protein, Oculorhombin

1 Images
Western blot - Anti-PAX6 antibody [PAX6/498] - BSA and Azide free (AB212694)
  • WB

Supplier Data

Western blot - Anti-PAX6 antibody [PAX6/498] - BSA and Azide free (AB212694)

All lanes:

Western blot - Anti-PAX6 antibody [PAX6/498] - BSA and Azide free (ab212694) at 1 µg/mL

All lanes:

Human cerebellum lysate

Predicted band size: 47 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

PAX6/498

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human PAX6 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

P26367

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
ab212694 is purified from Bioreactor Concentrate by Protein A/G.
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PAX6 protein also known as paired box 6 is a transcription factor involved in the development of eyes and neural tissues. It consists of a paired domain and a homeodomain collectively giving a molecular weight of approximately 48 kDa. This protein is highly expressed in developing ocular and central nervous tissues where it controls the expression of genes necessary for proper development. PAX6 is a widely studied marker in developmental biology due to its critical role in cellular differentiation and patterning processes.
Biological function summary

PAX6 acts as an important regulator in eye and neural development functioning alone and as part of larger complexes with other transcription factors. This role ensures the correct formation of ocular structures including the lens retina and cornea and neural elements by activating downstream targets important for cell fate decisions. The PAX6 protein intricately influences the genetic circuits that guide stem cell differentiation highlighting its importance in embryogenesis and organogenesis.

Pathways

PAX6 is pivotal in the Wnt signaling pathway and retinoic acid signaling. Its interaction within the Wnt pathway orchestrates vital developmental processes including those of eye morphogenesis and brain patterning. The protein collaborates with other transcriptional regulators such as SOX2 and EYA1 within these pathways facilitating the complex network of signals necessary for organ development and cellular identity specification.

PAX6 mutations have strong associations with aniridia and congenital eye malformations. Mutations in PAX6 disrupt the transcriptional regulation required for eye development often resulting in severe vision defects and structural anomalies. The intracacy of its involvement extends to disorders like Wilms' tumor wherein its altered expression or function in conjunction with proteins such as WT1 may contribute to oncogenesis. Scientists frequently use PAX6 staining to investigate these conditions and better understand their molecular underpinnings.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

PAX6 is a transcription factor with important roles in the development of the eye, nose, central nervous system, and pancreas. It competes with PAX4 for binding to a common element in the promoters of glucagon, insulin, and somatostatin. This supplementary information is collated from multiple sources and compiled automatically.
See full target information PAX6
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 9:18096 PubMed31792260

2019

BioSphincters to treat Fecal Incontinence in Nonhuman Primates.

Applications

Unspecified application

Species

Unspecified reactive species

Prabhash Dadhich,Jaime L Bohl,Riccardo Tamburrini,Elie Zakhem,Christie Scott,Nancy Kock,Erin Mitchell,John Gilliam,Khalil N Bitar
View all publications
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