Anti-PAX7 antibody ab187339 is a rabbit polyclonal antibody that is used in PAX7 western blotting and immunofluorescence. Suitable for human and mouse samples.
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- Science robotics 8:eadd93692023Anti-inflammatory therapy enables robot-actuated regeneration of aged muscle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesS L McNamara,B R Seo,B R Freedman,E B Roloson,J T Alvarez,C T O'Neill,H H Vandenburgh,C J Walsh,D J MooneyPubMed 36947599
- Frontiers in physiology 13:10444882022Chemokine-like receptor 1 plays a critical role in modulating the regenerative and contractile properties of muscle tissue.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulian Boesch,Eliane Pierrel,Christian Lambert,Arno Doelemeyer,Julie Kreider,Nathalie Accart,Serge SummermatterPubMed 36467705
- Nature materials 22:249-2592022Active tissue adhesive activates mechanosensors and prevents muscle atrophy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSungmin Nam,Bo Ri Seo,Alexander J Najibi,Stephanie L McNamara,David J MooneyPubMed 36357687
- Ophthalmic research 66:301-3062022Differences in Inferior Oblique Muscle Satellite Cell Populations between Primary and Secondary Inferior Oblique Muscle Overaction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui Hao,Yuchuan Wang,Liping Chen,Yang Liu,Wei ZhangPubMed 36302348
- Journal of cachexia, sarcopenia and muscle 13:1403-14172022Role of skeletal muscle satellite cells in the repair of osteoporotic fractures mediated by β-catenin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenxiong Jin,Weiwei Da,Yongjian Zhao,Tengteng Wang,Hao Xu,Bing Shu,Xiang Gao,Qi Shi,Yong Ma,Yan Zhang,Yongjun Wang,Dezhi TangPubMed 35178895
- Frontiers in cell and developmental biology 9:7853802021Transcriptome-wide -Methyladenosine Methylome Profiling Reveals mA Regulation of Skeletal Myoblast Differentiation in Cattle ().Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinran Yang,Jianfang Wang,Xinhao Ma,Jiawei Du,Chugang Mei,Linsen ZanPubMed 34938736
- Frontiers in endocrinology 12:6939772021An Study on Extracellular Vesicles From Adipose-Derived Mesenchymal Stem Cells in Protecting Stress Urinary Incontinence Through MicroRNA-93/F3 Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLu Wang,Yali Wang,Yuancui Xiang,Jinping Ma,Hui Zhang,Jingfang Dai,Yanan Hou,Yupei Yang,Jingru Ma,Hongjuan LiPubMed 34484115
- Advanced biology 5:e20002802021Milliscale Substrate Curvature Promotes Myoblast Self-Organization and Differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChe J Connon,Ricardo M GouveiaPubMed 33852180
- Stem cell research & therapy 12:1312021Pluripotent stem cell-induced skeletal muscle progenitor cells with givinostat promote myoangiogenesis and restore dystrophin in injured Duchenne dystrophic muscle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWanling Xuan,Mahmood Khan,Muhammad AshrafPubMed 33579366
- Scientific reports 10:176472020Association of advanced glycation end products with sarcopenia and frailty in chronic kidney disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunko Yabuuchi et. al.PubMed 33077879
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 69% PBS, 30% Glycerol (glycerin, glycerine), 0.1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PAX7. The exact immunogen used to generate this antibody is proprietary information. Database link P23759
Reactivity data
Select an application| ICC/IF | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 3 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/2000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
Transcription factor that is involved in the regulation of muscle stem cells proliferation, playing a role in myogenesis and muscle regeneration.
Alternative names
HUP1, HUP1, PAX7, Paired box protein Pax-7, HuP1
Recommended products
Anti-PAX7 antibody ab187339 is a rabbit polyclonal antibody that is used in PAX7 western blotting and immunofluorescence. Suitable for human and mouse samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 69% PBS, 30% Glycerol (glycerin, glycerine), 0.1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PAX7. The exact immunogen used to generate this antibody is proprietary information. Database link P23759
- Purification technique
Affinity purification Immunogen
- Specificity
Western blot analysis of ab187339 detects a prominent ~54 kDa protein in human HeLa, MCF7, rhabdomyosarcoma and skeletal muscle satellite cells and a weaker ~57 kDa protein in HeLa, rhabdomyosarcoma and skeletal muscle satellite cells. The ~57 kDa band seems to be enriched in immunoprecipitations from HeLa lysate, suggesting a bias for the larger isoform in ths application.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PAX7 or paired box 7 is a member of the paired box (PAX) family of transcription factors. Alternative names for PAX7 include PAX7 protein and PAX7 FACS. This protein has a molecular weight of approximately 57 kDa. PAX7 is mainly expressed in muscle precursor cells where it plays an important role in regulating muscle development. Researchers commonly detect it through PAX7 staining techniques in laboratory settings.
- Biological function summary
PAX7 functions are important for muscle cell proliferation and differentiation. It works within a transcriptional complex where it partners with other proteins to promote satellite cell maintenance. Satellite cells are essential for muscle regeneration and PAX7 regulates their self-renewal and differentiation. The protein also modulates various genetic targets that affect the muscle cellular environment ensuring proper muscle function and repair.
- Pathways
PAX7 is involved in the myogenic regulatory pathways that guide muscle development and regeneration. It interacts with important proteins like MyoD and Myf5 to influence these processes. These pathways facilitate the conversion of satellite cells into mature muscle fibers important during muscle growth and healing. PAX7’s function in these pathways ensures satellite cells remain active and capable of muscle repair throughout an organism's life.
- Associated diseases and disorders
PAX7’s dysfunction can lead to muscle-related conditions such as rhabdomyosarcoma and certain muscle dystrophies. In rhabdomyosarcoma improper regulation of PAX7 leads to unchecked cellular proliferation contributing to tumor growth. This disorder links PAX7 to other proteins in the tumorigenic pathway such as PAX3 which similarly influences muscle cell behavior in pathological states. Identifying PAX7 alterations can guide diagnosis and therapeutic strategies in muscle-associated disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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7 product images
Immunocytochemistry/ Immunofluorescence - Anti-PAX7 antibody (ab187339)
Immunocytochemistry analysis of formalin-fixed HeLa cells permeabilized with 0.1% Triton X-100 staining PAX-7 in the nucleus with ab187339 in a 1/100 dilution (green). Secondary was DyLight-conjugated secondary antibody. Negative control (left), had no primary antibody. F-actin was stianed with fluorescent red phalloidin, and nuclei were stained with DAPI or Hoechst.
Immunocytochemistry/ Immunofluorescence - Anti-PAX7 antibody (ab187339)
Immunocytochemistry analysis of formalin-fixed MCF-7 cells (human breast adenocarcinoma cell line) permeabilized with 0.1% Triton X-100 staining PAX-7 in the nucleus with ab187339 in a 1/100 dilution (green). Secondary was DyLight-conjugated secondary antibody. Negative control (left), had no primary antibody. F-actin was stianed with fluorescent red phalloidin, and nuclei were stained with DAPI or Hoechst.
Immunocytochemistry/ Immunofluorescence - Anti-PAX7 antibody (ab187339)
Immunocytochemistry analysis of formalin-fixed C2C12 cells (mouse myoblast cell line) permeabilized with 0.1% Triton X-100 staining PAX-7 in the nucleus with ab187339 in a 1/100 dilution (green). Secondary was DyLight-conjugated secondary antibody. Negative control (left), had no primary antibody. F-actin was stianed with fluorescent red phalloidin, and nuclei were stained with DAPI or Hoechst.
Western blot - Anti-PAX7 antibody (ab187339)
All lanes: Western blot - Anti-PAX7 antibody (ab187339) at 1/1000 dilution
Lane 1: HeLa lysate transfected with empty vector control at 50 µg
Lane 2: HeLa lysate overexpressing PAX7 at 50 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 56 kDa
Western blot - Anti-PAX7 antibody (ab187339)
All lanes: Western blot - Anti-PAX7 antibody (ab187339) at 1/1000 dilution
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: MCF7 whole cell lysate at 50 µg
Lane 3: HSkMSC whole cell lysate at 50 µg
Lane 4: SJCRH30 whole cell lysate at 50 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 56 kDa
Immunoprecipitation - Anti-PAX7 antibody (ab187339)
Immunoprecipitation of PAX7 was performed using HeLa whole cell lysate. Antigen-antibody complexes were formed by incubating 500μg of lysate with 3μg of ab187339 overnight on a rocking platform at 4°C. The immune complexes were captured on 50ul Protein A/G Agarose, washed extensively, and eluted. Samples was resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween-20 for 1 hour. The membrane was probed with ab187339 at 1/1000 dilution overnight rotating at 4°C.
All lanes: Immunoprecipitation - Anti-PAX7 antibody (ab187339)
Developed using the ECL technique.
Predicted band size: 56 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PAX7 antibody (ab187339)
Immunofluorescent analysis of PAX7 in MCF7 (left panel) and HeLa (right panel) cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were probed with a ab187339 at 1/100 dilution for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/400 dilution for 30 minutes at room temperature. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.
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