AB34360

Anti-PAX7 antibody

(5 Reviews)

(43 Publications)

Rabbit Polyclonal PAX7 antibody. Suitable for WB and reacts with Human samples. Cited in 43 publications.

View Alternative Names

HUP1, PAX7, Paired box protein Pax-7, HuP1

1 Images

Lab

Western blot - Anti-PAX7 antibody (AB34360)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab34360 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

All lanes:

Western blot - Anti-PAX7 antibody (ab34360) at 1 µg/mL

Lane 1:

Human Uterus Tissue Lysate (negative control) at 10 µg

Lane 2:

TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/5000 dilution

Predicted band size: 56 kDa

Observed band size: 37 kDa,65 kDa,90 kDa

true

Exposure time: 2min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PAX7 or paired box 7 is a member of the paired box (PAX) family of transcription factors. Alternative names for PAX7 include PAX7 protein and PAX7 FACS. This protein has a molecular weight of approximately 57 kDa. PAX7 is mainly expressed in muscle precursor cells where it plays an important role in regulating muscle development. Researchers commonly detect it through PAX7 staining techniques in laboratory settings.

Biological function summary

PAX7 functions are important for muscle cell proliferation and differentiation. It works within a transcriptional complex where it partners with other proteins to promote satellite cell maintenance. Satellite cells are essential for muscle regeneration and PAX7 regulates their self-renewal and differentiation. The protein also modulates various genetic targets that affect the muscle cellular environment ensuring proper muscle function and repair.

Pathways

PAX7 is involved in the myogenic regulatory pathways that guide muscle development and regeneration. It interacts with important proteins like MyoD and Myf5 to influence these processes. These pathways facilitate the conversion of satellite cells into mature muscle fibers important during muscle growth and healing. PAX7’s function in these pathways ensures satellite cells remain active and capable of muscle repair throughout an organism's life.

PAX7’s dysfunction can lead to muscle-related conditions such as rhabdomyosarcoma and certain muscle dystrophies. In rhabdomyosarcoma improper regulation of PAX7 leads to unchecked cellular proliferation contributing to tumor growth. This disorder links PAX7 to other proteins in the tumorigenic pathway such as PAX3 which similarly influences muscle cell behavior in pathological states. Identifying PAX7 alterations can guide diagnosis and therapeutic strategies in muscle-associated disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that is involved in the regulation of muscle stem cells proliferation, playing a role in myogenesis and muscle regeneration.

See full target information PAX7

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Publications (43)

Recent publications for all applications. Explore the full list and refine your search

PloS one 18:e0281718 PubMed36763621

2023

Metformin regulates myoblast differentiation through an AMPK-dependent mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Eleonora Maniscalco,Giuliana Abbadessa,Magalì Giordano,Loredana Grasso,Paolo Borrione,Silvia Racca

Nature 613:169-178 PubMed36544018

2022

Senescence atlas reveals an aged-like inflamed niche that blunts muscle regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria Moiseeva,Andrés Cisneros,Valentina Sica,Oleg Deryagin,Yiwei Lai,Sascha Jung,Eva Andrés,Juan An,Jessica Segalés,Laura Ortet,Vera Lukesova,Giacomo Volpe,Alberto Benguria,Ana Dopazo,Salvador Aznar Benitah,Yasuteru Urano,Antonio Del Sol,Miguel A Esteban,Yasuyuki Ohkawa,Antonio L Serrano,Eusebio Perdiguero,Pura Muñoz-Cánoves

NPJ science of food 6:41 PubMed36057641

2022

Production of scaffold-free cell-based meat using cell sheet technology.

Applications

Unspecified application

Species

Unspecified reactive species

Ryu-Ichiro Tanaka,Katsuhisa Sakaguchi,Azumi Yoshida,Hironobu Takahashi,Yuji Haraguchi,Tatsuya Shimizu

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e22401 PubMed35726676

2022

SNAI1 is upregulated during muscle regeneration and represses FGF21 and ATF3 expression by directly binding their promoters.

Applications

Unspecified application

Species

Unspecified reactive species

Ines Elia,Giulia Realini,Vittoria Di Mauro,Sara Borghi,Laura Bottoni,Salvatore Tornambè,Libero Vitiello,Stephen J Weiss,Mario Chiariello,Annalaura Tamburrini,Salvatore Oliviero,Francesco Neri,Maurizio Orlandini,Federico Galvagni

Bioengineering (Basel, Switzerland) 7: PubMed33105732

2020

Characterization of Functional Human Skeletal Myotubes and Neuromuscular Junction Derived-From the Same Induced Pluripotent Stem Cell Source.

Applications

Unspecified application

Species

Unspecified reactive species

Xiufang Guo,Agnes Badu-Mensah,Michael C Thomas,Christopher W McAleer,James J Hickman

Development (Cambridge, England) 147: PubMed32541004

2020

Differentiation of the human PAX7-positive myogenic precursors/satellite cell lineage .

Applications

Unspecified application

Species

Unspecified reactive species

Ziad Al Tanoury,Jyoti Rao,Olivier Tassy,Bénédicte Gobert,Svetlana Gapon,Jean-Marie Garnier,Erica Wagner,Aurore Hick,Arielle Hall,Emanuela Gussoni,Olivier Pourquié

American journal of physiology. Cell physiology 319:C116-C128 PubMed32374678

2020

Premature satellite cell activation before injury accelerates myogenesis and disrupts neuromuscular junction maturation in regenerating muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Nasibeh Daneshvar,Ryuichi Tatsumi,Jason Peeler,Judy E Anderson

Cells 9: PubMed32340154

2020

Satellite Cells in Skeletal Muscle of the Hibernating Dormouse, a Natural Model of Quiescence and Re-Activation: Focus on the Cell Nucleus.

Applications

Unspecified application

Species

Unspecified reactive species

Manuela Malatesta,Manuela Costanzo,Barbara Cisterna,Carlo Zancanaro

Acta physiologica (Oxford, England) 226:e13278 PubMed30943315

2019

miR-29c improves skeletal muscle mass and function throughout myocyte proliferation and differentiation and by repressing atrophy-related genes.

Applications

Unspecified application

Species

Unspecified reactive species

William José Silva,Flavia Aparecida Graça,André Cruz,João Guilherme Silvestre,Siegfried Labeit,Elen Haruka Miyabara,Chao Yun Irene Yan,Da Zhi Wang,Anselmo Sigari Moriscot

The Journal of physiology 597:889-901 PubMed30511745

2018

Lipin1 is required for skeletal muscle development by regulating MEF2c and MyoD expression.

Applications

Unspecified application

Species

Unspecified reactive species

Abdulrahman Jama,Dengtong Huang,Abdullah A Alshudukhi,Roman Chrast,Hongmei Ren

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PAX7 antibody

Western blot - Anti-PAX7 antibody (AB34360)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

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Secondary Antibodies

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Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (43)

Product promise