Anti-PAX7 antibody [EPR30466-531] (ab324017)

Rabbit Recombinant Monoclonal PAX7 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Transfected cell line - Mouse, Mouse, Human samples.

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Images

Western blot - Anti-PAX7 antibody [EPR30466-531] (AB324017), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	WB	ICC/IF
Human	Expected	Tested	Tested
Mouse	Tested	Tested	Not recommended
Transfected cell line - Mouse	Tested	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell line - Mouse	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell line - Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Transfected cell line - Mouse	Dilution info -	Notes -

Target data

See full target information Pax7

Function

Transcription factor that is involved in the regulation of muscle stem cells proliferation, playing a role in myogenesis and muscle regeneration.

Additional Targets

PAX7

Alternative names

Pax-7, Paired box protein Pax-7

Rabbit Recombinant Monoclonal PAX7 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Transfected cell line - Mouse, Mouse, Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR30466-531
Purification technique: Affinity purification Protein A
Specificity: Unsuitable for mouse ICC.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

Western blot - Anti-PAX7 antibody [EPR30466-531] (ab324017)
PAX7 Western blot staining using rabbit Anti-PAX7 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-PAX7 antibody [EPR30466-531] (ab324017) at 1/1000 dilution
Lane 1: C2C12 (mouse myoblast) transfected with scrambled siRNA control whole cell lysate at 20 µg
Lane 2: C2C12 transfected with siRNA specifically targeting PAX7 whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 54 kDa, 124 kDa
Exposure time: 95s
Western blot - Anti-PAX7 antibody [EPR30466-531] (ab324017)
PAX7 Western blot staining using rabbit Anti-PAX7 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: NIH/3T3 (PMID:11030621).
To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-PAX7 antibody [EPR30466-531] (ab324017) at 1/1000 dilution
Lane 1: C2C12 (mouse myoblast) whole cell lysate at 20 µg
Lane 2: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 54 kDa, 124 kDa
Exposure time: 81s
Western blot - Anti-PAX7 antibody [EPR30466-531] (ab324017)
PAX7 Western blot staining using rabbit Anti-PAX7 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: mouse testis (PMID:25133429)
To minimize protein degradation, tissues were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-PAX7 antibody [EPR30466-531] (ab324017) at 1/1000 dilution
Lane 1: Mouse E12 embryo tissue lysate at 60 µg
Lane 2: Mouse E15 brain tissue lysate at 60 µg
Lane 3: Mouse testis tissue lysate at 60 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 54 kDa, 124 kDa
Exposure time: 26s
Western blot - Anti-PAX7 antibody [EPR30466-531] (ab324017)
PAX7 Western blot staining using rabbit Anti-PAX7 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: mouse kidney (PMID:11030621).
To minimize protein degradation, tissues were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-PAX7 antibody [EPR30466-531] (ab324017) at 1/1000 dilution
Lane 1: Mouse E12-E14.5 brain tissue lysate at 20 µg
Lane 2: Mouse kidney tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 54 kDa, 124 kDa
Exposure time: 48s
Western blot - Anti-PAX7 antibody [EPR30466-531] (ab324017)
PAX7 Western blot staining using rabbit Anti-PAX7 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: HeLa (PMID:11030621).
To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-PAX7 antibody [EPR30466-531] (ab324017) at 1/1000 dilution
Lane 1: TT (human thyroid carcinoma epithelial cell) whole cell lysate at 60 µg
Lane 2: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 60 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 54 kDa, 124 kDa
Exposure time: 6s
Immunocytochemistry/ Immunofluorescence - Anti-PAX7 antibody [EPR30466-531] (ab324017)
PAX7 Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-PAX7 antibody
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized TT (human thyroid carcinoma epithelial cell) cells labelling PAX7 with ab324017 at 1/500 (0.994 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).
Confocal image showing nuclear staining in TT cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).

Low expression: HeLa.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [EPR30466-531] (ab324017)
PAX7 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse cerebrum using rabbit Anti-PAX7 antibody
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling PAX7 with ab324017 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in mouse cerebrum.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [EPR30466-531] (ab324017)
PAX7 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse E12.5 brain using rabbit Anti-PAX7 antibody
Immunohistochemical analysis of paraffin-embedded Mouse E12.5 brain tissue labeling PAX7 with ab324017 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse E12.5 brain.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [EPR30466-531] (ab324017)
PAX7 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse testis using rabbit Anti-PAX7 antibody
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling PAX7 with ab324017 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining in mouse testis (PMID:25133429).

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [EPR30466-531] (ab324017)
PAX7 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-PAX7 antibody
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a Mouse Pax7 expression vector containing a Myc-His tag and (B) HEK-293T transfected with empty vector containing a Myc-His tag labeling PAX7 with ab324017 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a Mouse Pax7 expression vector containing a Myc-His tag, no signal in (B) HEK-293T transfected with empty vector containing a Myc-His tag.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [EPR30466-531] (ab324017)
PAX7 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-PAX7 antibody
Immunohistochemical analysis of paraffin-embedded (A) C2C12 (mouse myoblast) cell pellets; (B) NIH/3T3 (mouse embryonic fibroblast) cell pellets labeling PAX7 with ab324017 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in (A) C2C12 (mouse myoblast) cell pellets, no signal in (B) NIH/3T3 (mouse embryonic fibroblast) cell pellets.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins