Anti-PAX7 antibody [EPR30466-531]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PAX7 antibody [EPR30466-531] (AB324017)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized TT (human thyroid carcinoma epithelial cell) cells labelling PAX7 with ab324017 at 1/500 (0.994 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

Confocal image showing nuclear staining in TT cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Low expression : HeLa.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [EPR30466-531] (AB324017)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a Mouse Pax7 expression vector containing a Myc-His tag and (B) HEK-293T transfected with empty vector containing a Myc-His tag labeling PAX7 with ab324017 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a Mouse Pax7 expression vector containing a Myc-His tag, no signal in (B) HEK-293T transfected with empty vector containing a Myc-His tag.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [EPR30466-531] (AB324017)

Immunohistochemical analysis of paraffin-embedded Mouse E12.5 brain tissue labeling PAX7 with ab324017 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse E12.5 brain.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [EPR30466-531] (AB324017)

Immunohistochemical analysis of paraffin-embedded (A) C2C12 (mouse myoblast) cell pellets; (B) NIH/3T3 (mouse embryonic fibroblast) cell pellets labeling PAX7 with ab324017 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in (A) C2C12 (mouse myoblast) cell pellets, no signal in (B) NIH/3T3 (mouse embryonic fibroblast) cell pellets.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [EPR30466-531] (AB324017)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling PAX7 with ab324017 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in mouse testis (PMID : 25133429).
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [EPR30466-531] (AB324017)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling PAX7 with ab324017 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in mouse cerebrum.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-PAX7 antibody [EPR30466-531] (AB324017)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : HeLa (PMID : 11030621).

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-PAX7 antibody [EPR30466-531] (ab324017) at 1/1000 dilution

Lane 1:

TT (human thyroid carcinoma epithelial cell) whole cell lysate at 60 µg

Lane 2:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 60 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 54 kDa,124 kDa

false

Exposure time: 6s

• WB

Supplier Data

Western blot - Anti-PAX7 antibody [EPR30466-531] (AB324017)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : mouse kidney (PMID : 11030621).

To minimize protein degradation, tissues were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-PAX7 antibody [EPR30466-531] (ab324017) at 1/1000 dilution

Lane 1:

Mouse E12-E14.5 brain tissue lysate at 20 µg

Lane 2:

Mouse kidney tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 54 kDa,124 kDa

false

Exposure time: 48s

• WB

Supplier Data

Western blot - Anti-PAX7 antibody [EPR30466-531] (AB324017)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : mouse testis (PMID : 25133429)

To minimize protein degradation, tissues were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-PAX7 antibody [EPR30466-531] (ab324017) at 1/1000 dilution

Lane 1:

Mouse E12 embryo tissue lysate at 60 µg

Lane 2:

Mouse E15 brain tissue lysate at 60 µg

Lane 3:

Mouse testis tissue lysate at 60 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 54 kDa,124 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Anti-PAX7 antibody [EPR30466-531] (AB324017)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : NIH/3T3 (PMID : 11030621).

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-PAX7 antibody [EPR30466-531] (ab324017) at 1/1000 dilution

Lane 1:

C2C12 (mouse myoblast) whole cell lysate at 20 µg

Lane 2:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 54 kDa,124 kDa

false

Exposure time: 81s

• WB

Supplier Data

Western blot - Anti-PAX7 antibody [EPR30466-531] (AB324017)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-PAX7 antibody [EPR30466-531] (ab324017) at 1/1000 dilution

Lane 1:

C2C12 (mouse myoblast) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

C2C12 transfected with siRNA specifically targeting PAX7 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 54 kDa,124 kDa

false

Exposure time: 95s