Rabbit Recombinant Monoclonal PAX7 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Transfected cell line - Mouse, Mouse, Human samples.
Images
![Western blot - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (AB324018), expandable thumbnail](https://content.abcam.com/products/images/pax7-antibody-epr30466-531-bsa-and-azide-free-ab324018--western-blot-img446176.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (AB324018)")
![Western blot - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (AB324018), expandable thumbnail](https://content.abcam.com/products/images/pax7-antibody-epr30466-531-bsa-and-azide-free-ab324018--western-blot-img446177.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (AB324018)")
![Western blot - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (AB324018), expandable thumbnail](https://content.abcam.com/products/images/pax7-antibody-epr30466-531-bsa-and-azide-free-ab324018--western-blot-img446178.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (AB324018)")
![Western blot - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (AB324018), expandable thumbnail](https://content.abcam.com/products/images/pax7-antibody-epr30466-531-bsa-and-azide-free-ab324018--western-blot-img446179.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (AB324018)")
![Western blot - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (AB324018), expandable thumbnail](https://content.abcam.com/products/images/pax7-antibody-epr30466-531-bsa-and-azide-free-ab324018--western-blot-img446180.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (AB324018)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Expected | Tested | Tested |
| Mouse | Tested | Tested | Not recommended |
| Transfected cell line - Mouse | Tested | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Transfected cell line - Mouse | Dilution info - | Notes - |
Target data
Function
Transcription factor that is involved in the regulation of muscle stem cells proliferation, playing a role in myogenesis and muscle regeneration.
Additional Targets
PAX7
Alternative names
Pax-7, Paired box protein Pax-7
Rabbit Recombinant Monoclonal PAX7 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF and reacts with Transfected cell line - Mouse, Mouse, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR30466-531
- Purification technique
- Affinity purification Protein A
- Specificity
Unsuitable for mouse ICC.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab324018 is the carrier free version of Anti-PAX7 antibody [EPR30466-531] ab324017.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
11 product images
![Western blot - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018), expandable thumbnail](https://content.abcam.com/products/images/pax7-antibody-epr30466-531-bsa-and-azide-free-ab324018--western-blot-img446176.jpg "Western blot - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018)")
Western blot - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018)
PAX7 Western blot staining using rabbit Anti-PAX7 antibody
This data was developed using Anti-PAX7 antibody [EPR30466-531] ab324017, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-PAX7 antibody [EPR30466-531] (Anti-PAX7 antibody [EPR30466-531] ab324017) at 1/1000 dilution
Lane 1: C2C12 (mouse myoblast) transfected with scrambled siRNA control whole cell lysate at 20 µg
Lane 2: C2C12 transfected with siRNA specifically targeting PAX7 whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 54 kDa, 124 kDa
Exposure time: 95s
![Western blot - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018), expandable thumbnail](https://content.abcam.com/products/images/pax7-antibody-epr30466-531-bsa-and-azide-free-ab324018--western-blot-img446177.jpg "Western blot - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018)")
Western blot - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018)
PAX7 Western blot staining using rabbit Anti-PAX7 antibody
This data was developed using Anti-PAX7 antibody [EPR30466-531] ab324017, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: NIH/3T3 (PMID:11030621).
To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-PAX7 antibody [EPR30466-531] (Anti-PAX7 antibody [EPR30466-531] ab324017) at 1/1000 dilution
Lane 1: C2C12 (mouse myoblast) whole cell lysate at 20 µg
Lane 2: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 54 kDa, 124 kDa
Exposure time: 81s
![Western blot - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018), expandable thumbnail](https://content.abcam.com/products/images/pax7-antibody-epr30466-531-bsa-and-azide-free-ab324018--western-blot-img446178.jpg "Western blot - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018)")
Western blot - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018)
PAX7 Western blot staining using rabbit Anti-PAX7 antibody
This data was developed using Anti-PAX7 antibody [EPR30466-531] ab324017, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: mouse testis (PMID:25133429)
To minimize protein degradation, tissues were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-PAX7 antibody [EPR30466-531] (Anti-PAX7 antibody [EPR30466-531] ab324017) at 1/1000 dilution
Lane 1: Mouse E12 embryo tissue lysate at 60 µg
Lane 2: Mouse E15 brain tissue lysate at 60 µg
Lane 3: Mouse testis tissue lysate at 60 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 54 kDa, 124 kDa
Exposure time: 26s
![Western blot - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018), expandable thumbnail](https://content.abcam.com/products/images/pax7-antibody-epr30466-531-bsa-and-azide-free-ab324018--western-blot-img446179.jpg "Western blot - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018)")
Western blot - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018)
PAX7 Western blot staining using rabbit Anti-PAX7 antibody
This data was developed using Anti-PAX7 antibody [EPR30466-531] ab324017, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: mouse kidney (PMID:11030621).
To minimize protein degradation, tissues were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-PAX7 antibody [EPR30466-531] (Anti-PAX7 antibody [EPR30466-531] ab324017) at 1/1000 dilution
Lane 1: Mouse E12-E14.5 brain tissue lysate at 20 µg
Lane 2: Mouse kidney tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 54 kDa, 124 kDa
Exposure time: 48s
![Western blot - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018), expandable thumbnail](https://content.abcam.com/products/images/pax7-antibody-epr30466-531-bsa-and-azide-free-ab324018--western-blot-img446180.jpg "Western blot - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018)")
Western blot - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018)
PAX7 Western blot staining using rabbit Anti-PAX7 antibody
This data was developed using Anti-PAX7 antibody [EPR30466-531] ab324017, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: HeLa (PMID:11030621).
To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-PAX7 antibody [EPR30466-531] (Anti-PAX7 antibody [EPR30466-531] ab324017) at 1/1000 dilution
Lane 1: TT (human thyroid carcinoma epithelial cell) whole cell lysate at 60 µg
Lane 2: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 60 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 54 kDa, 124 kDa
Exposure time: 6s
![Immunocytochemistry/ Immunofluorescence - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018), expandable thumbnail](https://content.abcam.com/products/images/pax7-antibody-epr30466-531-bsa-and-azide-free-ab324018--immunocytochemistry-immunofluorescence-img446189.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018)")
Immunocytochemistry/ Immunofluorescence - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018)
PAX7 Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-PAX7 antibody
This data was developed using Anti-PAX7 antibody [EPR30466-531] ab324017, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized TT (human thyroid carcinoma epithelial cell) cells labelling PAX7 with Anti-PAX7 antibody [EPR30466-531] ab324017 at 1/500 (0.994 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).
Confocal image showing nuclear staining in TT cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Low expression: HeLa.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018), expandable thumbnail](https://content.abcam.com/products/images/pax7-antibody-epr30466-531-bsa-and-azide-free-ab324018--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446227.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018)
PAX7 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse cerebrum using rabbit Anti-PAX7 antibody
This data was developed using Anti-PAX7 antibody [EPR30466-531] ab324017, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling PAX7 with Anti-PAX7 antibody [EPR30466-531] ab324017 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in mouse cerebrum.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018), expandable thumbnail](https://content.abcam.com/products/images/pax7-antibody-epr30466-531-bsa-and-azide-free-ab324018--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446228.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018)
PAX7 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse E12.5 brain using rabbit Anti-PAX7 antibody
This data was developed using Anti-PAX7 antibody [EPR30466-531] ab324017, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse E12.5 brain tissue labeling PAX7 with Anti-PAX7 antibody [EPR30466-531] ab324017 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse E12.5 brain.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018), expandable thumbnail](https://content.abcam.com/products/images/pax7-antibody-epr30466-531-bsa-and-azide-free-ab324018--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446229.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018)
PAX7 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse testis using rabbit Anti-PAX7 antibody
This data was developed using Anti-PAX7 antibody [EPR30466-531] ab324017, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling PAX7 with Anti-PAX7 antibody [EPR30466-531] ab324017 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining in mouse testis (PMID:25133429).
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018), expandable thumbnail](https://content.abcam.com/products/images/pax7-antibody-epr30466-531-bsa-and-azide-free-ab324018--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446230.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018)
PAX7 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-PAX7 antibody
This data was developed using Anti-PAX7 antibody [EPR30466-531] ab324017, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a Mouse Pax7 expression vector containing a Myc-His tag and (B) HEK-293T transfected with empty vector containing a Myc-His tag labeling PAX7 with Anti-PAX7 antibody [EPR30466-531] ab324017 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a Mouse Pax7 expression vector containing a Myc-His tag, no signal in (B) HEK-293T transfected with empty vector containing a Myc-His tag.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018), expandable thumbnail](https://content.abcam.com/products/images/pax7-antibody-epr30466-531-bsa-and-azide-free-ab324018--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446231.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018)
PAX7 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-PAX7 antibody
This data was developed using Anti-PAX7 antibody [EPR30466-531] ab324017, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) C2C12 (mouse myoblast) cell pellets; (B) NIH/3T3 (mouse embryonic fibroblast) cell pellets labeling PAX7 with Anti-PAX7 antibody [EPR30466-531] ab324017 at 1/2000 (0.249 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in (A) C2C12 (mouse myoblast) cell pellets, no signal in (B) NIH/3T3 (mouse embryonic fibroblast) cell pellets.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunocytochemistry/ Immunofluorescence - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018), expandable thumbnail](https://content.abcam.com/products/images/pax7-antibody-epr30466-531-bsa-and-azide-free-ab324018--immunocytochemistry-immunofluorescence-img446189.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018), expandable thumbnail](https://content.abcam.com/products/images/pax7-antibody-epr30466-531-bsa-and-azide-free-ab324018--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446227.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018), expandable thumbnail](https://content.abcam.com/products/images/pax7-antibody-epr30466-531-bsa-and-azide-free-ab324018--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446228.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018), expandable thumbnail](https://content.abcam.com/products/images/pax7-antibody-epr30466-531-bsa-and-azide-free-ab324018--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img446229.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [EPR30466-531] - BSA and Azide free (ab324018)")
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