AB269804

Anti-PAX7 antibody [SPM613] - BSA and Azide free

Name: Anti-PAX7 antibody [SPM613] - BSA and Azide free (ab269804) | Abcam
Brand: Abcam Limited
SKU: AB269804
Price: 905 USD
Availability: InStock

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Mouse Monoclonal PAX7 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PAX7 aa 300 to C-terminus.

View Alternative Names

HUP1, PAX7, Paired box protein Pax-7, HuP1

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [SPM613] - BSA and Azide free (AB269804)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [SPM613] - BSA and Azide free (AB269804)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human rhabdomyosarcoma tissue section labeling PAX7 with ab218473 at 1 μg/mL.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218473)

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

SPM613

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PAX7 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P23759

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p>Primary incubation is for 30 min at RT.</p>" } } }

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Product details

ab269804 is a carrier free version of ab218473.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PAX7 or paired box 7 is a member of the paired box (PAX) family of transcription factors. Alternative names for PAX7 include PAX7 protein and PAX7 FACS. This protein has a molecular weight of approximately 57 kDa. PAX7 is mainly expressed in muscle precursor cells where it plays an important role in regulating muscle development. Researchers commonly detect it through PAX7 staining techniques in laboratory settings.

Biological function summary

PAX7 functions are important for muscle cell proliferation and differentiation. It works within a transcriptional complex where it partners with other proteins to promote satellite cell maintenance. Satellite cells are essential for muscle regeneration and PAX7 regulates their self-renewal and differentiation. The protein also modulates various genetic targets that affect the muscle cellular environment ensuring proper muscle function and repair.

Pathways

PAX7 is involved in the myogenic regulatory pathways that guide muscle development and regeneration. It interacts with important proteins like MyoD and Myf5 to influence these processes. These pathways facilitate the conversion of satellite cells into mature muscle fibers important during muscle growth and healing. PAX7’s function in these pathways ensures satellite cells remain active and capable of muscle repair throughout an organism's life.

PAX7’s dysfunction can lead to muscle-related conditions such as rhabdomyosarcoma and certain muscle dystrophies. In rhabdomyosarcoma improper regulation of PAX7 leads to unchecked cellular proliferation contributing to tumor growth. This disorder links PAX7 to other proteins in the tumorigenic pathway such as PAX3 which similarly influences muscle cell behavior in pathological states. Identifying PAX7 alterations can guide diagnosis and therapeutic strategies in muscle-associated disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that is involved in the regulation of muscle stem cells proliferation, playing a role in myogenesis and muscle regeneration.

See full target information PAX7

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PAX7 antibody [SPM613] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [SPM613] - BSA and Azide free (AB269804)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise