Mouse Monoclonal PAX7 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PAX7 aa 300 to C-terminus.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [SPM613] - BSA and Azide free (AB269804), expandable thumbnail](https://content.abcam.com/products/images/pax7-antibody-spm613-bsa-and-azide-free-ab269804--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136875.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [SPM613] - BSA and Azide free (AB269804)")
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Recombinant Fragment Protein within Human PAX7 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P23759
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-1 µg/mL | Notes Primary incubation is for 30 min at RT. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
1 products for Alternative Version
5 products for Alternative Product
Target data
Function
Transcription factor that is involved in the regulation of muscle stem cells proliferation, playing a role in myogenesis and muscle regeneration.
Alternative names
Paired box protein Pax-7, HuP1, HUP1, PAX7
Recommended products
Mouse Monoclonal PAX7 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PAX7 aa 300 to C-terminus.
Alternative names
Paired box protein Pax-7, HuP1, HUP1, PAX7
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human PAX7 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P23759
- Carrier free
Yes
- Clone number
SPM613
- Purification technique
Affinity purification Protein G
- Light chain type
kappa
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
ab269804 is a carrier free version of Anti-PAX7 antibody [SPM613] ab218473.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PAX7 or paired box 7 is a member of the paired box (PAX) family of transcription factors. Alternative names for PAX7 include PAX7 protein and PAX7 FACS. This protein has a molecular weight of approximately 57 kDa. PAX7 is mainly expressed in muscle precursor cells where it plays an important role in regulating muscle development. Researchers commonly detect it through PAX7 staining techniques in laboratory settings.
- Biological function summary
PAX7 functions are important for muscle cell proliferation and differentiation. It works within a transcriptional complex where it partners with other proteins to promote satellite cell maintenance. Satellite cells are essential for muscle regeneration and PAX7 regulates their self-renewal and differentiation. The protein also modulates various genetic targets that affect the muscle cellular environment ensuring proper muscle function and repair.
- Pathways
PAX7 is involved in the myogenic regulatory pathways that guide muscle development and regeneration. It interacts with important proteins like MyoD and Myf5 to influence these processes. These pathways facilitate the conversion of satellite cells into mature muscle fibers important during muscle growth and healing. PAX7’s function in these pathways ensures satellite cells remain active and capable of muscle repair throughout an organism's life.
- Associated diseases and disorders
PAX7’s dysfunction can lead to muscle-related conditions such as rhabdomyosarcoma and certain muscle dystrophies. In rhabdomyosarcoma improper regulation of PAX7 leads to unchecked cellular proliferation contributing to tumor growth. This disorder links PAX7 to other proteins in the tumorigenic pathway such as PAX3 which similarly influences muscle cell behavior in pathological states. Identifying PAX7 alterations can guide diagnosis and therapeutic strategies in muscle-associated disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [SPM613] - BSA and Azide free (ab269804), expandable thumbnail](https://content.abcam.com/products/images/pax7-antibody-spm613-bsa-and-azide-free-ab269804--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136875.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [SPM613] - BSA and Azide free (ab269804)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX7 antibody [SPM613] - BSA and Azide free (ab269804)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human rhabdomyosarcoma tissue section labeling PAX7 with Anti-PAX7 antibody [SPM613] ab218473 at 1 µg/mL.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAX7 antibody [SPM613] ab218473)
.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com