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Mouse Monoclonal PAX8 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, African green monkey, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human PAX8.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [1F8-3A8] (AB183573), expandable thumbnail
  • Western blot - Anti-PAX8 antibody [1F8-3A8] (AB183573), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody [1F8-3A8] (AB183573), expandable thumbnail
  • Western blot - Anti-PAX8 antibody [1F8-3A8] (AB183573), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

Preservative: 0.05% Sodium azide
Constituents: 69% PBS, 30% Glycerol (glycerin, glycerine), 0.1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human PAX8. The exact immunogen used to generate this antibody is proprietary information. Database link Q06710

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Expected
Tested
Mouse
Expected
Tested
Expected
African green monkey
Tested
Expected
Tested
Primates
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/500 - 1/2000
Notes

-

Species
African green monkey
Dilution info
1/500 - 1/2000
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Primates
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/20 - 1/100
Notes

-

Expected
Expected

Species
Human, African green monkey
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Primates
Dilution info
-
Notes

-

Tested
Tested

Species
African green monkey
Dilution info
1/50 - 1/200
Notes

-

Species
Human
Dilution info
1/50 - 1/200
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Primates
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.

Alternative names

Paired box protein Pax-8, PAX8

Recommended products

Mouse Monoclonal PAX8 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, African green monkey, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human PAX8.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human PAX8. The exact immunogen used to generate this antibody is proprietary information. Database link Q06710
Clone number
1F8-3A8
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PAX8 also known as 3a8 is a transcription factor with a molecular mass of approximately 48 kDa. It belongs to the paired box (PAX) family of proteins which are characterized by a specific DNA-binding domain. PAX8 plays an important role in organ development and cellular differentiation. This protein is mainly expressed in the thyroid kidneys and Müllerian-derived tissues. Researchers often detect PAX8 using immunohistochemistry (PAX8 IHC) techniques due to its tissue-specific expression patterns.

Biological function summary

The PAX8 protein regulates the expression of genes related to organogenesis and cell fate determination. It forms part of transcriptional regulatory complexes partnering with other proteins to modulate gene activity. PAX8 supports embryonic development especially in the thyroid and urogenital tract. Its presence ensures the proper function and formation of these organs highlighting its significance in developmental biology.

Pathways

PAX8 interacts with several key biological processes. It notably participates in the thyroid hormone synthesis pathway where it regulates the expression of thyroglobulin and thyroperoxidase. PAX8 also affects the renal system through its interaction with WT1 a protein involved in kidney development. These pathways illustrate PAX8's role in maintaining endocrine and renal functions by influencing important protein interactions.

Associated diseases and disorders

PAX8 mutations and dysregulation connect to conditions like congenital hypothyroidism and renal agenesis. In congenital hypothyroidism defects in PAX8 lead to impaired thyroid development affecting hormone production. Moreover its interaction with BCL2 an important apoptosis regulator relates to certain cancers such as ovarian carcinoma. The misregulation of PAX8 increases the risk and progression of these disorders by disrupting normal physiological processes.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [1F8-3A8] (ab183573), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [1F8-3A8] (ab183573)

    Immunohistochemistry analysis of PAX8 showing staining in the nucleus of paraffin-embedded human kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab183573 diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Western blot - Anti-PAX8 antibody [1F8-3A8] (ab183573), expandable thumbnail

    Western blot - Anti-PAX8 antibody [1F8-3A8] (ab183573)

    4-20% Tris-HCl polyacrylamide gel. Blocked with 5% BSA/TBST for at least 1 hour.

    All lanes: Western blot - Anti-PAX8 antibody [1F8-3A8] (ab183573) at 1/1000 dilution

    Lane 1: SKOV-3 cell lysate at 60 µg

    Lane 2: COS7 cell lysate at 60 µg

    Lane 3: Negative control HeLa whole cell lysates at 60 µg

    Secondary

    All lanes: HRP-conjugated goat anti-mouse IgG at 1/500 dilution

    Predicted band size: 48 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody [1F8-3A8] (ab183573), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody [1F8-3A8] (ab183573)

    Immunofluorescent analysis of PAX8 (green) in 293T (left panel) and COS7 cells (right panel) using ab183573 at a 1/50 dilution. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% blocker BSA for 15 minutes at room temperature. A DyLight 488-conjugated goat anti-mouse IgG secondary antibody was used at a dilution of 1/400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.

  • Western blot - Anti-PAX8 antibody [1F8-3A8] (ab183573), expandable thumbnail

    Western blot - Anti-PAX8 antibody [1F8-3A8] (ab183573)

    4-20% Tris-HCl polyacrylamide gel. Blocked with 5% BSA/TBST for at least 1 hour.

    All lanes: Western blot - Anti-PAX8 antibody [1F8-3A8] (ab183573) at 1/1000 dilution

    Lane 1: untransfected HEK293T cell lysate at 25 µg

    Lane 2: HEK293T cell lysate over expressing PAX8 at 25 µg

    Secondary

    All lanes: HRP-conjugated goat anti-mouse IgG at 1/500 dilution

    Predicted band size: 48 kDa

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Product protocols

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