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AB97477

Anti-PAX8 antibody

4

(2 Reviews)

|

(10 Publications)

Rabbit Polyclonal PAX8 antibody. Suitable for IP, ChIP, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat, Dog samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PAX8 aa 1-250.

View Alternative Names

Paired box protein Pax-8, PAX8

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody (AB97477)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody (AB97477)

Immunofluorescence analysis of paraformaldehyde-fixed A549 (human lung carcinoma cell line) whole cells lableing PAX8 with ab97477 at 1/200 dilution. Bottom : Costained with DNA probe stain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (AB97477)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (AB97477)

Immunohistochemical analysis of paraffin-embedded DLD1 xenograft tissue staining PAX8 protein at nucleolus with ab97477 at 1/500 dilution.

Antigen Retrieval : EDTA based buffer, pH 8.0, 15min.

ChIP - Anti-PAX8 antibody (AB97477)
  • ChIP

Supplier Data

ChIP - Anti-PAX8 antibody (AB97477)

PAX8 antibody immunoprecipitates PAX8 protein-DNA in ChIP experiments.

ChIP Sample : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate;

A. 5 μg preimmune rabbit IgG;

B. 5 μg of ab97477.

The precipitated DNA was detected by PCR with primer set targeting to E2F1 promoter.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (AB97477)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (AB97477)

Immunohistochemical analysis of paraffin-embedded rat thyroid gland tissue staining PAX8 protein at cytosol with ab97477 at 1/500 dilution.

Antigen Retrieval : EDTA based buffer, pH 8.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (AB97477)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (AB97477)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue staining PAX8 protein at nucleus with ab97477 at 1/500 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (AB97477)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (AB97477)

Immunohistochemical analysis of paraffin-embedded dog kidney tissue stained for PAX8 protein at nucleus with ab97477 at a 1/2000 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min .

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human, Dog

Applications

IP, ChIP, IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PAX8 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q06710

Specificity

<p>Based on sequence homology, this antibody may cross react with PAX1, PAX2, PAX5 or PAX6 protein.</p>

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/500 - 1/1000", "IP-species-notes": "<p></p>", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PAX8 also known as 3a8 is a transcription factor with a molecular mass of approximately 48 kDa. It belongs to the paired box (PAX) family of proteins which are characterized by a specific DNA-binding domain. PAX8 plays an important role in organ development and cellular differentiation. This protein is mainly expressed in the thyroid kidneys and Müllerian-derived tissues. Researchers often detect PAX8 using immunohistochemistry (PAX8 IHC) techniques due to its tissue-specific expression patterns.
Biological function summary

The PAX8 protein regulates the expression of genes related to organogenesis and cell fate determination. It forms part of transcriptional regulatory complexes partnering with other proteins to modulate gene activity. PAX8 supports embryonic development especially in the thyroid and urogenital tract. Its presence ensures the proper function and formation of these organs highlighting its significance in developmental biology.

Pathways

PAX8 interacts with several key biological processes. It notably participates in the thyroid hormone synthesis pathway where it regulates the expression of thyroglobulin and thyroperoxidase. PAX8 also affects the renal system through its interaction with WT1 a protein involved in kidney development. These pathways illustrate PAX8's role in maintaining endocrine and renal functions by influencing important protein interactions.

PAX8 mutations and dysregulation connect to conditions like congenital hypothyroidism and renal agenesis. In congenital hypothyroidism defects in PAX8 lead to impaired thyroid development affecting hormone production. Moreover its interaction with BCL2 an important apoptosis regulator relates to certain cancers such as ovarian carcinoma. The misregulation of PAX8 increases the risk and progression of these disorders by disrupting normal physiological processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.
See full target information PAX8
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Reproductive biology and endocrinology : RB&E 23:47 PubMed40140889

2025

A novel senotherapeutic strategy with azithromycin for preventing endometriosis progression.

Applications

Unspecified application

Species

Unspecified reactive species

Reina Sonehara,Tomoko Nakamura,Takehiko Takeda,Satoshi Kaseki,Tomomi Seki,Hideaki Tanaka,Atsushi Yabuki,Natsuki Miyake,Ayako Muraoka,Satoko Osuka,Akira Iwase,Hiroaki Kajiyama

Antioxidants (Basel, Switzerland) 13: PubMed39594548

2024

An In Vitro Oxidative Stress Model of the Human Inner Ear Using Human-Induced Pluripotent Stem Cell-Derived Otic Progenitor Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Minjin Jeong,Sho Kurihara,Konstantina M Stankovic

Clinical and translational medicine 14:e1694 PubMed38797942

2024

Targeting miR-31 represses tumourigenesis and dedifferentiation of BRAF-associated thyroid carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Peitao Zhang,Lizhao Guan,Wei Sun,Yu Zhang,Yaying Du,Shukai Yuan,Xiaolong Cao,Zhengquan Yu,Qiang Jia,Xiangqian Zheng,Zhaowei Meng,Xingrui Li,Li Zhao

Cell stem cell 30:950-961.e7 PubMed37419105

2023

Generating high-fidelity cochlear organoids from human pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Stephen T Moore,Takashi Nakamura,Jing Nie,Alexander J Solivais,Isabel Aristizábal-Ramírez,Yoshitomo Ueda,Mayakannan Manikandan,V Shweta Reddy,Daniel R Romano,John R Hoffman,Benjamin J Perrin,Rick F Nelson,Gregory I Frolenkov,Susana M Chuva de Sousa Lopes,Eri Hashino

Frontiers in cell and developmental biology 9:602197 PubMed33968919

2021

Activin/Nodal/TGF-β Pathway Inhibitor Accelerates BMP4-Induced Cochlear Gap Junction Formation During Differentiation of Embryonic Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ichiro Fukunaga,Yoko Oe,Cheng Chen,Keiko Danzaki,Sayaka Ohta,Akito Koike,Katsuhisa Ikeda,Kazusaku Kamiya

Molecular and cellular biochemistry 476:1751-1763 PubMed33433832

2021

The circular RNA circSlc7a11 promotes bone cancer pain pathogenesis in rats by modulating LLC-WRC 256 cell proliferation and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Han-Wen Chen,Xiao-Xia Zhang,Zhu-Ding Peng,Zu-Min Xing,Yi-Wen Zhang,Ya-Lan Li

Frontiers in cellular neuroscience 11:409 PubMed29311837

2017

Generation of Otic Sensory Neurons from Mouse Embryonic Stem Cells in 3D Culture.

Applications

ICC/IF

Species

Unspecified reactive species

Michael Perny,Ching-Chia Ting,Sonja Kleinlogel,Pascal Senn,Marta Roccio

PloS one 11:e0162508 PubMed27607106

2016

Modulation of Wnt Signaling Enhances Inner Ear Organoid Development in 3D Culture.

Applications

IHC-P

Species

Unspecified reactive species

Rachel E DeJonge,Xiao-Ping Liu,Christopher R Deig,Stefan Heller,Karl R Koehler,Eri Hashino

Thyroid : official journal of the American Thyroid 25:455-61 PubMed25585054

2015

Human embryonic stem cells form functional thyroid follicles.

Applications

Unspecified application

Species

Unspecified reactive species

Risheng Ma,Rauf Latif,Terry F Davies

Nature protocols 9:1229-44 PubMed24784820

2014

3D mouse embryonic stem cell culture for generating inner ear organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Karl R Koehler,Eri Hashino
View all publications
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Product promise

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