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Rabbit Polyclonal PAX8 antibody. Suitable for IP, ChIP, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PAX8 aa 1-250.


Images

ChIP - Anti-PAX8 antibody (AB97477), expandable thumbnail
  • Western blot - Anti-PAX8 antibody (AB97477), expandable thumbnail
  • Immunoprecipitation - Anti-PAX8 antibody (AB97477), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (AB97477), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody (AB97477), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human PAX8 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q06710

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPChIPWBIHC-PICC/IF
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Expected
Expected
Expected
Tested
Expected
Rat
Expected
Expected
Expected
Tested
Expected

Tested
Tested

Species

Human

Dilution info

1/500 - 1/1000

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500 - 1/2000

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100 - 1/1000

Notes

-

Species

Rat

Dilution info

1/100 - 1/1000

Notes

-

Species

Human

Dilution info

1/100 - 1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/1000

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.

Alternative names

Recommended products

Rabbit Polyclonal PAX8 antibody. Suitable for IP, ChIP, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PAX8 aa 1-250.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human PAX8 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q06710
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PAX8 also known as 3a8 is a transcription factor with a molecular mass of approximately 48 kDa. It belongs to the paired box (PAX) family of proteins which are characterized by a specific DNA-binding domain. PAX8 plays an important role in organ development and cellular differentiation. This protein is mainly expressed in the thyroid kidneys and Müllerian-derived tissues. Researchers often detect PAX8 using immunohistochemistry (PAX8 IHC) techniques due to its tissue-specific expression patterns.

Biological function summary

The PAX8 protein regulates the expression of genes related to organogenesis and cell fate determination. It forms part of transcriptional regulatory complexes partnering with other proteins to modulate gene activity. PAX8 supports embryonic development especially in the thyroid and urogenital tract. Its presence ensures the proper function and formation of these organs highlighting its significance in developmental biology.

Pathways

PAX8 interacts with several key biological processes. It notably participates in the thyroid hormone synthesis pathway where it regulates the expression of thyroglobulin and thyroperoxidase. PAX8 also affects the renal system through its interaction with WT1 a protein involved in kidney development. These pathways illustrate PAX8's role in maintaining endocrine and renal functions by influencing important protein interactions.

Associated diseases and disorders

PAX8 mutations and dysregulation connect to conditions like congenital hypothyroidism and renal agenesis. In congenital hypothyroidism defects in PAX8 lead to impaired thyroid development affecting hormone production. Moreover its interaction with BCL2 an important apoptosis regulator relates to certain cancers such as ovarian carcinoma. The misregulation of PAX8 increases the risk and progression of these disorders by disrupting normal physiological processes.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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8 product images

  • ChIP - Anti-PAX8 antibody (ab97477), expandable thumbnail

    ChIP - Anti-PAX8 antibody (ab97477)

    PAX8 antibody immunoprecipitates PAX8 protein-DNA in ChIP experiments.

    ChIP Sample: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate;

    A. 5 μg preimmune rabbit IgG;

    B. 5 μg of ab97477.

    The precipitated DNA was detected by PCR with primer set targeting to E2F1 promoter.

  • Western blot - Anti-PAX8 antibody (ab97477), expandable thumbnail

    Western blot - Anti-PAX8 antibody (ab97477)

    10% SDS-PAGE

    All lanes: Western blot - Anti-PAX8 antibody (ab97477) at 1/10000 dilution

    Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

    Lane 2: DDDDK-tagged PAX8 transfected HEK-293T whole cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 48 kDa

  • Immunoprecipitation - Anti-PAX8 antibody (ab97477), expandable thumbnail

    Immunoprecipitation - Anti-PAX8 antibody (ab97477)

    ab97477 immunoprecipitates PAX8 protein in IP experiments.

    IP Sample: 1000 μg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

    1- 40 μg HEK-293T whole cell lysate;

    2- Control with 2 μg of preimmune rabbit IgG;

    3- Immunoprecipitation of PAX8 protein by 2 μg ofab97477.

    10% SDS-PAGE

    The immunoprecipitated PAX8 protein was detected byab97477 at 1/1000 dilution, followed by anti-rabbit IgG antibody.

    All lanes: Immunoprecipitation - Anti-PAX8 antibody (ab97477)

    Predicted band size: 48 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (ab97477), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (ab97477)

    Immunohistochemical analysis of paraffin-embedded DLD1 xenograft tissue staining PAX8 protein at nucleolus with ab97477 at 1/500 dilution.

    Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.

  • Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody (ab97477), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody (ab97477)

    Immunofluorescence analysis of paraformaldehyde-fixed A549 (human lung carcinoma cell line) whole cells lableing PAX8 with ab97477 at 1/200 dilution. Bottom: Costained with DNA probe stain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (ab97477), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (ab97477)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue staining PAX8 protein at nucleus with ab97477 at 1/500 dilution.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

  • Western blot - Anti-PAX8 antibody (ab97477), expandable thumbnail

    Western blot - Anti-PAX8 antibody (ab97477)

    10% SDS-PAGE

    All lanes: Western blot - Anti-PAX8 antibody (ab97477) at 1/1000 dilution

    Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

    Lane 2: HEK-293T cell nuclear extract at 30 µg

    Predicted band size: 48 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (ab97477), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody (ab97477)

    Immunohistochemical analysis of paraffin-embedded rat thyroid gland tissue staining PAX8 protein at cytosol with ab97477 at 1/500 dilution.

    Antigen Retrieval:EDTA based buffer, pH 8.0, 15 min.

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Product protocols

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