Rabbit Polyclonal PAX8 antibody. Suitable for IP, ChIP, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PAX8 aa 1-250.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
IP | ChIP | WB | IHC-P | ICC/IF | |
---|---|---|---|---|---|
Human | Tested | Tested | Tested | Tested | Tested |
Mouse | Expected | Expected | Expected | Tested | Expected |
Rat | Expected | Expected | Expected | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 - 1/2000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes - |
Species Rat | Dilution info 1/100 - 1/1000 | Notes - |
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Select an associated product type
Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.
Paired box protein Pax-8, PAX8
Rabbit Polyclonal PAX8 antibody. Suitable for IP, ChIP, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PAX8 aa 1-250.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
PAX8 also known as 3a8 is a transcription factor with a molecular mass of approximately 48 kDa. It belongs to the paired box (PAX) family of proteins which are characterized by a specific DNA-binding domain. PAX8 plays an important role in organ development and cellular differentiation. This protein is mainly expressed in the thyroid kidneys and Müllerian-derived tissues. Researchers often detect PAX8 using immunohistochemistry (PAX8 IHC) techniques due to its tissue-specific expression patterns.
The PAX8 protein regulates the expression of genes related to organogenesis and cell fate determination. It forms part of transcriptional regulatory complexes partnering with other proteins to modulate gene activity. PAX8 supports embryonic development especially in the thyroid and urogenital tract. Its presence ensures the proper function and formation of these organs highlighting its significance in developmental biology.
PAX8 interacts with several key biological processes. It notably participates in the thyroid hormone synthesis pathway where it regulates the expression of thyroglobulin and thyroperoxidase. PAX8 also affects the renal system through its interaction with WT1 a protein involved in kidney development. These pathways illustrate PAX8's role in maintaining endocrine and renal functions by influencing important protein interactions.
PAX8 mutations and dysregulation connect to conditions like congenital hypothyroidism and renal agenesis. In congenital hypothyroidism defects in PAX8 lead to impaired thyroid development affecting hormone production. Moreover its interaction with BCL2 an important apoptosis regulator relates to certain cancers such as ovarian carcinoma. The misregulation of PAX8 increases the risk and progression of these disorders by disrupting normal physiological processes.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Terms & Conditions.
PAX8 antibody immunoprecipitates PAX8 protein-DNA in ChIP experiments.
ChIP Sample: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate;
A. 5 μg preimmune rabbit IgG;
B. 5 μg of ab97477.
The precipitated DNA was detected by PCR with primer set targeting to E2F1 promoter.
10% SDS-PAGE
All lanes: Western blot - Anti-PAX8 antibody (ab97477) at 1/10000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Lane 2: DDDDK-tagged PAX8 transfected HEK-293T whole cell lysate at 30 µg
All lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 48 kDa
ab97477 immunoprecipitates PAX8 protein in IP experiments.
IP Sample: 1000 μg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
1- 40 μg HEK-293T whole cell lysate;
2- Control with 2 μg of preimmune rabbit IgG;
3- Immunoprecipitation of PAX8 protein by 2 μg ofab97477.
10% SDS-PAGE
The immunoprecipitated PAX8 protein was detected byab97477 at 1/1000 dilution, followed by anti-rabbit IgG antibody.
All lanes: Immunoprecipitation - Anti-PAX8 antibody (ab97477)
Predicted band size: 48 kDa
Immunohistochemical analysis of paraffin-embedded DLD1 xenograft tissue staining PAX8 protein at nucleolus with ab97477 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
Immunofluorescence analysis of paraformaldehyde-fixed A549 (human lung carcinoma cell line) whole cells lableing PAX8 with ab97477 at 1/200 dilution. Bottom: Costained with DNA probe stain.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue staining PAX8 protein at nucleus with ab97477 at 1/500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
10% SDS-PAGE
All lanes: Western blot - Anti-PAX8 antibody (ab97477) at 1/1000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Lane 2: HEK-293T cell nuclear extract at 30 µg
Predicted band size: 48 kDa
Immunohistochemical analysis of paraffin-embedded rat thyroid gland tissue staining PAX8 protein at cytosol with ab97477 at 1/500 dilution.
Antigen Retrieval:EDTA based buffer, pH 8.0, 15 min.
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