Anti-PAX8 antibody [EPR13510]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR13510] (AB181054)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid tissue labelling PAX8 with purified ab181054 at a dilution of 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051 a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR13510] (AB181054)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling PAX8 with unpurified ab181054 at a dilution of 1/500 followed by incubation with a pre-diluted HRP-conjugated secondary antibody and counter-staining with Hematoxylin.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR13510] (AB181054)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid tissue labelling PAX8 with unpurified ab181054 at a dilution of 1/500 followed by incubation with a pre-diluted HRP-conjugated secondary antibody and counter-staining with Hematoxylin.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-PAX8 antibody [EPR13510] (AB181054)

Blocking/Diluting buffer : 5% NFDM/TBST

Loading Control : Rabbit monoclonal [EPR16891] to GAPDH (ab181602)

All lanes:

Western blot - Anti-PAX8 antibody [EPR13510] (ab181054) at 1/1000 dilution

Lane 1:

SK-OV-3 (Human ovarian cancer epithelial cell) cell lysate at 10 µg

Lane 2:

TT (Human thyroid carcinoma epithelial cell) cell lysate at 10 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 4:

Human kidney lysate at 10 µg

Lane 5:

Human heart lysate at 10 µg

Lane 6:

Human thyroid lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 48 kDa

Observed band size: 30 kDa,50 kDa

false

Exposure time: 7s

• ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR13510] (AB181054)

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 NIH : OVCAR-3 (Human ovary adenocarcinoma epithelial cell) cells and 5 µg of ab181054 [EPR13510]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

• ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR13510] (AB181054)

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 NIH : OVCAR-3 (Human ovary adenocarcinoma epithelial cell) cells and 5 µg of ab181054 [EPR13510]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods."

• ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR13510] (AB181054)

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 NIH : OVCAR-3 (Human ovary adenocarcinoma epithelial cell) cells and 5 µg of ab181054 [EPR13510]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods."