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Rabbit Recombinant Monoclonal PAX8 antibody. Suitable for IHC-P, WB, ChIC/CUT&RUN-seq and reacts with Human samples. Cited in 1 publication.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR13510] (AB181054), expandable thumbnail
  • Western blot - Anti-PAX8 antibody [EPR13510] (AB181054), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR13510] (AB181054), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR13510] (AB181054), expandable thumbnail
  • ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR13510] (AB181054), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBChIC/CUT&RUN-seq
Human
Tested
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/250 - 1/1000
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Associated Products

Select an associated product type

14 products for Alternative Product

Target data

Function

Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.

Alternative names

Paired box protein Pax-8, PAX8

Recommended products

Rabbit Recombinant Monoclonal PAX8 antibody. Suitable for IHC-P, WB, ChIC/CUT&RUN-seq and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR13510
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PAX8 also known as 3a8 is a transcription factor with a molecular mass of approximately 48 kDa. It belongs to the paired box (PAX) family of proteins which are characterized by a specific DNA-binding domain. PAX8 plays an important role in organ development and cellular differentiation. This protein is mainly expressed in the thyroid kidneys and Müllerian-derived tissues. Researchers often detect PAX8 using immunohistochemistry (PAX8 IHC) techniques due to its tissue-specific expression patterns.

Biological function summary

The PAX8 protein regulates the expression of genes related to organogenesis and cell fate determination. It forms part of transcriptional regulatory complexes partnering with other proteins to modulate gene activity. PAX8 supports embryonic development especially in the thyroid and urogenital tract. Its presence ensures the proper function and formation of these organs highlighting its significance in developmental biology.

Pathways

PAX8 interacts with several key biological processes. It notably participates in the thyroid hormone synthesis pathway where it regulates the expression of thyroglobulin and thyroperoxidase. PAX8 also affects the renal system through its interaction with WT1 a protein involved in kidney development. These pathways illustrate PAX8's role in maintaining endocrine and renal functions by influencing important protein interactions.

Associated diseases and disorders

PAX8 mutations and dysregulation connect to conditions like congenital hypothyroidism and renal agenesis. In congenital hypothyroidism defects in PAX8 lead to impaired thyroid development affecting hormone production. Moreover its interaction with BCL2 an important apoptosis regulator relates to certain cancers such as ovarian carcinoma. The misregulation of PAX8 increases the risk and progression of these disorders by disrupting normal physiological processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR13510] (ab181054), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR13510] (ab181054)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid tissue labelling PAX8 with purified ab181054 at a dilution of 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Western blot - Anti-PAX8 antibody [EPR13510] (ab181054), expandable thumbnail

    Western blot - Anti-PAX8 antibody [EPR13510] (ab181054)

    Blocking/Diluting buffer: 5% NFDM/TBST

    Loading Control: Rabbit monoclonal [EPR16891] to GAPDH (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602)

    All lanes: Western blot - Anti-PAX8 antibody [EPR13510] (ab181054) at 1/1000 dilution

    Lane 1: SK-OV-3 (Human ovarian cancer epithelial cell) cell lysate at 10 µg

    Lane 2: TT (Human thyroid carcinoma epithelial cell) cell lysate at 10 µg

    Lane 3: 293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

    Lane 4: Human kidney lysate at 10 µg

    Lane 5: Human heart lysate at 10 µg

    Lane 6: Human thyroid lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 48 kDa

    Observed band size: 30 kDa, 50 kDa

    Exposure time: 7s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR13510] (ab181054), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR13510] (ab181054)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid tissue labelling PAX8 with unpurified ab181054 at a dilution of 1/500 followed by incubation with a pre-diluted HRP-conjugated secondary antibody and counter-staining with Hematoxylin.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR13510] (ab181054), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR13510] (ab181054)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling PAX8 with unpurified ab181054 at a dilution of 1/500 followed by incubation with a pre-diluted HRP-conjugated secondary antibody and counter-staining with Hematoxylin.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR13510] (ab181054), expandable thumbnail

    ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR13510] (ab181054)

    ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) cells and 5 µg of ab181054 [EPR13510]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

  • ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR13510] (ab181054), expandable thumbnail

    ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR13510] (ab181054)

    ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) cells and 5 µg of ab181054 [EPR13510]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods."

  • ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR13510] (ab181054), expandable thumbnail

    ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR13510] (ab181054)

    ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) cells and 5 µg of ab181054 [EPR13510]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods."

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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