Rabbit Recombinant Monoclonal PAX8 antibody. C-terminal. Suitable for IHC-P and reacts with Human samples. Cited in 15 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR13511] - C-terminal (AB189249), expandable thumbnail](https://content.abcam.com/products/images/pax8-antibody-epr13511-c-terminal-ab189249--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img69742.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR13511] - C-terminal (AB189249)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR13511] - C-terminal (AB189249), expandable thumbnail](https://content.abcam.com/products/images/pax8-antibody-epr13511-c-terminal-ab189249--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133711.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR13511] - C-terminal (AB189249)")
Publications
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- Cell stem cell 29:1083-1101.e72022A scalable organoid model of human autosomal dominant polycystic kidney disease for disease mechanism and drug discovery.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTracy Tran,Cheng Jack Song,Trang Nguyen,Shun-Yang Cheng,Jill A McMahon,Rui Yang,Qiuyu Guo,Balint Der,Nils O Lindström,Daniel C-H Lin,Andrew P McMahonPubMed 35803227
- Cancers 13:2021Patient-Derived Xenografts of High-Grade Serous Ovarian Cancer Subtype as a Powerful Tool in Pre-Clinical Research.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMagdalena Cybula,Lin Wang,Luyao Wang,Ana Luiza Drumond-Bock,Katherine M Moxley,Doris M Benbrook,Camille Gunderson-Jackson,Maria J Ruiz-Echevarria,Resham Bhattacharya,Priyabrata Mukherjee,Magdalena BieniaszPubMed 34944908
- Developmental cell 56:2381-2398.e62021Spatial transcriptional mapping of the human nephrogenic program.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNils O Lindström,Rachel Sealfon,Xi Chen,Riana K Parvez,Andrew Ransick,Guilherme De Sena Brandine,Jinjin Guo,Bill Hill,Tracy Tran,Albert D Kim,Jian Zhou,Alicja Tadych,Aaron Watters,Aaron Wong,Elizabeth Lovero,Brendan H Grubbs,Matthew E Thornton,Jill A McMahon,Andrew D Smith,Seth W Ruffins,Chris Armit,Olga G Troyanskaya,Andrew P McMahonPubMed 34428401
- STAR protocols 2:1003142021regeneration of neo-nephrons in rodents by renal progenitor cell transplantation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuichiro Yamanaka et. al.PubMed 33554147
- Cell reports 32:1081302020Generation of Human Renal Vesicles in Mouse Organ Niche Using Nephron Progenitor Cell Replacement System.Applications:Unspecified applicationReactive species:Unspecified reactive speciesToshinari Fujimoto,Shuichiro Yamanaka,Susumu Tajiri,Tsuyoshi Takamura,Yatsumu Saito,Naoto Matsumoto,Kei Matsumoto,Toshiaki Tachibana,Hirotaka James Okano,Takashi YokooPubMed 32937125
- BMC research notes 13:3962020Immunohistochemical expression of PAX-8 in Sudanese patients diagnosed with malignant female reproductive tract tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEman T Ali et. al.PubMed 32847623
- Genes & cancer 11:106-1212020Multi-kinase targeted therapy as a promising treatment strategy for ovarian tumors expressing sfRon receptor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuyao Wang et. al.PubMed 33488949
- Scientific reports 10:98372020WNT and inflammatory signaling distinguish human Fallopian tube epithelial cell populations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIan M Rose et. al.PubMed 32555344
- JCI insight 4:2019Disruption of Robo2-Baiap2 integrated signaling drives cystic disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQinggang Li et. al.PubMed 31534052
- Developmental cell 50:102-116.e62019In Vivo Developmental Trajectories of Human Podocyte Inform In Vitro Differentiation of Pluripotent Stem Cell-Derived Podocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTracy Tran,Nils O Lindström,Andrew Ransick,Guilherme De Sena Brandine,Qiuyu Guo,Albert D Kim,Balint Der,Janos Peti-Peterdi,Andrew D Smith,Matthew Thornton,Brendan Grubbs,Jill A McMahon,Andrew P McMahonPubMed 31265809
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Not recommended | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Predicted |
| Rat | Not recommended | Not recommended | Predicted |
| Dog | Not recommended | Not recommended | Predicted |
| Orangutan | Not recommended | Not recommended | Predicted |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
Species Dog | Dilution info - | Notes - |
Species Orangutan | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Dog, Orangutan | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 - 1/10000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Dog, Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
13 products for Alternative Product
Target data
Function
Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.
Alternative names
Paired box protein Pax-8, PAX8
Recommended products
Rabbit Recombinant Monoclonal PAX8 antibody. C-terminal. Suitable for IHC-P and reacts with Human samples. Cited in 15 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR13511
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PAX8 also known as 3a8 is a transcription factor with a molecular mass of approximately 48 kDa. It belongs to the paired box (PAX) family of proteins which are characterized by a specific DNA-binding domain. PAX8 plays an important role in organ development and cellular differentiation. This protein is mainly expressed in the thyroid kidneys and Müllerian-derived tissues. Researchers often detect PAX8 using immunohistochemistry (PAX8 IHC) techniques due to its tissue-specific expression patterns.
- Biological function summary
The PAX8 protein regulates the expression of genes related to organogenesis and cell fate determination. It forms part of transcriptional regulatory complexes partnering with other proteins to modulate gene activity. PAX8 supports embryonic development especially in the thyroid and urogenital tract. Its presence ensures the proper function and formation of these organs highlighting its significance in developmental biology.
- Pathways
PAX8 interacts with several key biological processes. It notably participates in the thyroid hormone synthesis pathway where it regulates the expression of thyroglobulin and thyroperoxidase. PAX8 also affects the renal system through its interaction with WT1 a protein involved in kidney development. These pathways illustrate PAX8's role in maintaining endocrine and renal functions by influencing important protein interactions.
- Associated diseases and disorders
PAX8 mutations and dysregulation connect to conditions like congenital hypothyroidism and renal agenesis. In congenital hypothyroidism defects in PAX8 lead to impaired thyroid development affecting hormone production. Moreover its interaction with BCL2 an important apoptosis regulator relates to certain cancers such as ovarian carcinoma. The misregulation of PAX8 increases the risk and progression of these disorders by disrupting normal physiological processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR13511] - C-terminal (ab189249), expandable thumbnail](https://content.abcam.com/products/images/pax8-antibody-epr13511-c-terminal-ab189249--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img69742.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR13511] - C-terminal (ab189249)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR13511] - C-terminal (ab189249)
Nuclear staining on paraffin-embedded sections of human thyroid carcinoma tissue, performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. Samples were incubated with primary antibody at a 1:10000 dilution (0.1 μg/ml). A Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as the counterstain. A secondary antibody-only control was also performed.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR13511] - C-terminal (ab189249), expandable thumbnail](https://content.abcam.com/products/images/pax8-antibody-epr13511-c-terminal-ab189249--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133711.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR13511] - C-terminal (ab189249)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR13511] - C-terminal (ab189249)
Immunohistochemical analysis of formalin fixed paraffin embedded human thyroid carcinoma labeling PAX8 with ab189249 at a 1/500 dilution and HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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