AB189249

Anti-PAX8 antibody [EPR13511] - C-terminal

Name: Anti-PAX8 antibody - C-terminal [EPR13511] (ab189249) | Abcam
Brand: Abcam Limited
SKU: AB189249
Price: 250 USD
Availability: InStock
Rating: 4 (1 reviews)

20ul selling size
BOND RX™ Validated
RabMAb
Recombinant
What is this?

(1 Review)

(21 Publications)

Rabbit Recombinant Monoclonal PAX8 antibody. C-terminal. Suitable for IHC-P and reacts with Human samples. Cited in 21 publications.

View Alternative Names

Paired box protein Pax-8, PAX8

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR13511] - C-terminal (AB189249)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR13511] - C-terminal (AB189249)

Nuclear staining on paraffin-embedded sections of human thyroid carcinoma tissue, performed on a Leica Biosystems BOND^® RX instrument. Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. Samples were incubated with primary antibody at a 1 : 10000 dilution (0.1 μg/ml). A Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as the counterstain. A secondary antibody-only control was also performed.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR13511] - C-terminal (AB189249)

Immunohistochemical analysis of formalin fixed paraffin embedded human thyroid carcinoma labeling PAX8 with ab189249 at a 1/500 dilution and HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Carrier free

Anti-PAX8 antibody [EPR13511] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13511

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/10000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PAX8 also known as 3a8 is a transcription factor with a molecular mass of approximately 48 kDa. It belongs to the paired box (PAX) family of proteins which are characterized by a specific DNA-binding domain. PAX8 plays an important role in organ development and cellular differentiation. This protein is mainly expressed in the thyroid kidneys and Müllerian-derived tissues. Researchers often detect PAX8 using immunohistochemistry (PAX8 IHC) techniques due to its tissue-specific expression patterns.

Biological function summary

The PAX8 protein regulates the expression of genes related to organogenesis and cell fate determination. It forms part of transcriptional regulatory complexes partnering with other proteins to modulate gene activity. PAX8 supports embryonic development especially in the thyroid and urogenital tract. Its presence ensures the proper function and formation of these organs highlighting its significance in developmental biology.

Pathways

PAX8 interacts with several key biological processes. It notably participates in the thyroid hormone synthesis pathway where it regulates the expression of thyroglobulin and thyroperoxidase. PAX8 also affects the renal system through its interaction with WT1 a protein involved in kidney development. These pathways illustrate PAX8's role in maintaining endocrine and renal functions by influencing important protein interactions.

PAX8 mutations and dysregulation connect to conditions like congenital hypothyroidism and renal agenesis. In congenital hypothyroidism defects in PAX8 lead to impaired thyroid development affecting hormone production. Moreover its interaction with BCL2 an important apoptosis regulator relates to certain cancers such as ovarian carcinoma. The misregulation of PAX8 increases the risk and progression of these disorders by disrupting normal physiological processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.

See full target information PAX8

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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8136 PubMed40885711

2025

Controlling nephron precursor differentiation to generate proximal-biased kidney organoids with emerging maturity.

Applications

Unspecified application

Species

Unspecified reactive species

Jack Schnell,Zhen Miao,MaryAnne Achieng,Connor C Fausto,Kari Koppitch,Lola Takhirov,Victoria Wang,Faith De Kuyper,Biao Huang,Megan Schreiber,Pedro Medina,Matthew E Thornton,Brendan Grubbs,Zhongwei Li,Junhyong Kim,Nils O Lindström

Nature communications 16:7912 PubMed40855070

2025

Axial nephron fate switching demonstrates a plastic system tunable on demand.

Applications

Unspecified application

Species

Unspecified reactive species

MaryAnne A Achieng,Jack Schnell,Connor C Fausto,Réka L Csipán,Kari Koppitch,Matthew E Thornton,Brendan H Grubbs,Nils O Lindström

Nature communications 16:4930 PubMed40436842

2025

An RNA transmethylation pathway governs kidney nephrogenic potential.

Applications

Unspecified application

Species

Unspecified reactive species

Harini Ramalingam,Jesus Alvarez,Andrea Flaten,Patricia Cobo-Stark,Nicholas Foster,Elyse Grilli,Ronak Lakhia,Karam Aboudehen,Thomas Carroll,Vishal Patel

Developmental cell 59:595-612.e8 PubMed38340720

2024

Identification of a core transcriptional program driving the human renal mesenchymal-to-epithelial transition.

Applications

Unspecified application

Species

Unspecified reactive species

John-Poul Ng-Blichfeldt,Benjamin J Stewart,Menna R Clatworthy,Julie M Williams,Katja Röper

Life medicine 2:lnad047 PubMed39872058

2023

Generation of renal tubular organoids from adult SOX9 kidney progenitor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dewei Zhou,Dandan Li,Hao Nie,Jun Duan,Sarah Liu,Yujia Wang,Wei Zuo

Cell reports. Medicine 4:101055 PubMed37220750

2023

Spatial and temporal intra-tumoral heterogeneity in advanced HGSOC: Implications for surgical and clinical outcomes.

Applications

Unspecified application

Species

Unspecified reactive species

Paula Cunnea,Edward W Curry,Elizabeth L Christie,Katherine Nixon,Chun Hei Kwok,Ahwan Pandey,Ratri Wulandari,Kerstin Thol,Jennifer Ploski,Cristina Morera-Albert,Stephen McQuaid,Jingky Lozano-Kuehne,James J Clark,Jonathan Krell,Euan A Stronach,Iain A McNeish,David D L Bowtell,Christina Fotopoulou

Cell stem cell 29:1083-1101.e7 PubMed35803227

2022

A scalable organoid model of human autosomal dominant polycystic kidney disease for disease mechanism and drug discovery.

Applications

Unspecified application

Species

Unspecified reactive species

Tracy Tran,Cheng Jack Song,Trang Nguyen,Shun-Yang Cheng,Jill A McMahon,Rui Yang,Qiuyu Guo,Balint Der,Nils O Lindström,Daniel C-H Lin,Andrew P McMahon

Cancers 13: PubMed34944908

2021

Patient-Derived Xenografts of High-Grade Serous Ovarian Cancer Subtype as a Powerful Tool in Pre-Clinical Research.

Applications

Unspecified application

Species

Unspecified reactive species

Magdalena Cybula,Lin Wang,Luyao Wang,Ana Luiza Drumond-Bock,Katherine M Moxley,Doris M Benbrook,Camille Gunderson-Jackson,Maria J Ruiz-Echevarria,Resham Bhattacharya,Priyabrata Mukherjee,Magdalena Bieniasz

Developmental cell 56:2381-2398.e6 PubMed34428401

2021

Spatial transcriptional mapping of the human nephrogenic program.

Applications

Unspecified application

Species

Unspecified reactive species

Nils O Lindström,Rachel Sealfon,Xi Chen,Riana K Parvez,Andrew Ransick,Guilherme De Sena Brandine,Jinjin Guo,Bill Hill,Tracy Tran,Albert D Kim,Jian Zhou,Alicja Tadych,Aaron Watters,Aaron Wong,Elizabeth Lovero,Brendan H Grubbs,Matthew E Thornton,Jill A McMahon,Andrew D Smith,Seth W Ruffins,Chris Armit,Olga G Troyanskaya,Andrew P McMahon

STAR protocols 2:100314 PubMed33554147

2021

regeneration of neo-nephrons in rodents by renal progenitor cell transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Shuichiro Yamanaka,Kenji Matsui,Toshinari Fujimoto,Tsuyoshi Takamura,Yatsumu Saito,Naoto Matsumoto,Susumu Tajiri,Kei Matsumoto,Takashi Yokoo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PAX8 antibody [EPR13511] - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR13511] - C-terminal (AB189249)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR13511] - C-terminal (AB189249)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (21)

Product promise