Anti-PAX8 antibody [EPR18715] (ab191870)

Anti-PAX8 antibody [EPR18715] (ab191870) is a rabbit monoclonal antibody detecting PAX8 in Western Blot, IHC-P, IHC-Fr, ICC/IF. Suitable for Human, Mouse.

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Images

Immunohistochemistry (Frozen sections) - Anti-PAX8 antibody [EPR18715] (AB191870), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	ICC/IF	IHC-Fr	IHC-P	ChIC/CUT&RUN-seq
Human	Tested	Tested	Expected	Tested	Tested
Mouse	Tested	Expected	Tested	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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Target data

See full target information PAX8

Function

Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.

Alternative names

Paired box protein Pax-8, PAX8

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR18715
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-PAX8 antibody [EPR18715] (ab191870) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.

What is the molecular weight of PAX8?

Anti-PAX8 [EPR18715] (ab191870) specifically detects a band for PAX8 (UniProt: Q06710) at a molecular weight of 48kDa.

Trusted by the scientific community

Anti-PAX8 [EPR18715] (ab191870) was first used in a scientific publication in 2015 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products

We have a range of other formats of antibody clone [EPR18715] also available for your convenience:
ab191870, Alexa Fluor® 488 - ab214955, Alexa Fluor® 647 - ab215953, Alexa Fluor® 555 - ab217733, Carrier free - ab246332

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PAX8 also known as 3a8 is a transcription factor with a molecular mass of approximately 48 kDa. It belongs to the paired box (PAX) family of proteins which are characterized by a specific DNA-binding domain. PAX8 plays an important role in organ development and cellular differentiation. This protein is mainly expressed in the thyroid kidneys and Mьllerian-derived tissues. Researchers often detect PAX8 using immunohistochemistry (PAX8 IHC) techniques due to its tissue-specific expression patterns.

Biological function summary

The PAX8 protein regulates the expression of genes related to organogenesis and cell fate determination. It forms part of transcriptional regulatory complexes partnering with other proteins to modulate gene activity. PAX8 supports embryonic development especially in the thyroid and urogenital tract. Its presence ensures the proper function and formation of these organs highlighting its significance in developmental biology.

Pathways

PAX8 interacts with several key biological processes. It notably participates in the thyroid hormone synthesis pathway where it regulates the expression of thyroglobulin and thyroperoxidase. PAX8 also affects the renal system through its interaction with WT1 a protein involved in kidney development. These pathways illustrate PAX8's role in maintaining endocrine and renal functions by influencing important protein interactions.

Associated diseases and disorders

PAX8 mutations and dysregulation connect to conditions like congenital hypothyroidism and renal agenesis. In congenital hypothyroidism defects in PAX8 lead to impaired thyroid development affecting hormone production. Moreover its interaction with BCL2 an important apoptosis regulator relates to certain cancers such as ovarian carcinoma. The misregulation of PAX8 increases the risk and progression of these disorders by disrupting normal physiological processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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10 product images

Immunohistochemistry (Frozen sections) - Anti-PAX8 antibody [EPR18715] (ab191870)
Immunohistochemistry (Frozen sections) analysis of mouse kidney tissue labelling PAX8 with ab191870 at a dilution of 1/500. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/1000). Nuclear staining is visible in the mouse kidney.
Western blot - Anti-PAX8 antibody [EPR18715] (ab191870)
Blocking/Dilution buffer: 5% NFDM/TBST.
PAX8 is expressed in the excretory system and thyroid gland. (PMID: 1723950, 9590297, 1069301)
All lanes: Western blot - Anti-PAX8 antibody [EPR18715] (ab191870) at 1/1000 dilution
Lane 1: Human thyroid cancer lysate at 10 µg
Lane 2: Human fetal liver lysate at 10 µg
Lane 3: Human fetal heart lysate at 10 µg
Lane 4: Human fetal kidney lysate at 10 µg
Lane 5: Human fetal spleen lysate at 10 µg
Secondary
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 3min
Western blot - Anti-PAX8 antibody [EPR18715] (ab191870)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-PAX8 antibody [EPR18715] (ab191870) at 1/1000 dilution
Lane 1: NIH:OVCAR-3 (Human ovary adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 2: SK-OV-3 (Human ovarian cancer cell line) whole cell lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 3min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR18715] (ab191870)
Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling PAX8 with ab191870 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nucleus staining on tumor cells of thyroid carcinoma is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR18715] (ab191870)
Immunohistochemical analysis of paraffin-embedded Human endometrium carcinoma tissue labeling PAX8 with ab191870 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nucleus staining on tumor cells of the endometrium carcinoma is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody [EPR18715] (ab191870)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH:OVCAR-3 (Human ovary adenocarcinoma cell line) cells labeling PAX8 with ab191870 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nucleus staining on NIH:OVCAR-3 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab191870 at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody [EPR18715] (ab191870)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-OV-3 (Human ovarian cancer cell line) cells labeling PAX8 with ab191870 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nucleus staining on SK-OV-3 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab191870 at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR18715] (ab191870)
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) cells and 5 µg of ab191870 [EPR18715]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods."
ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR18715] (ab191870)
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) cells and 5 µg of ab191870 [EPR18715]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods."
ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR18715] (ab191870)
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) cells and 5 µg of ab191870 [EPR18715]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods."