Rabbit Recombinant Monoclonal PAX8 antibody. Carrier free. Suitable for WB, ICC/IF, IHC-Fr, IHC-P, ChIC/CUT&RUN-seq and reacts with Mouse, Human samples.
Images
![Immunohistochemistry (Frozen sections) - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (AB246332), expandable thumbnail](https://content.abcam.com/products/images/pax8-antibody-epr18715-bsa-and-azide-free-ab246332--immunohistochemistry-frozen-sections-img108961.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (AB246332)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (AB246332), expandable thumbnail](https://content.abcam.com/products/images/pax8-antibody-epr18715-bsa-and-azide-free-ab246332--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img86315.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (AB246332)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (AB246332), expandable thumbnail](https://content.abcam.com/products/images/pax8-antibody-epr18715-bsa-and-azide-free-ab246332--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img33484.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (AB246332)")
![Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (AB246332), expandable thumbnail](https://content.abcam.com/products/images/pax8-antibody-epr18715-bsa-and-azide-free-ab246332--immunocytochemistry-immunofluorescence-img67810.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (AB246332)")
![Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (AB246332), expandable thumbnail](https://content.abcam.com/products/images/pax8-antibody-epr18715-bsa-and-azide-free-ab246332--immunocytochemistry-immunofluorescence-img68332.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (AB246332)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | IHC-Fr | IHC-P | ChIC/CUT&RUN-seq | |
|---|---|---|---|---|---|
| Human | Expected | Tested | Expected | Tested | Tested |
| Mouse | Expected | Expected | Tested | Expected | Expected |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ChIC/CUT&RUN-seq
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
11 products for Alternative Product
3 products for Alternative Version
Target data
Function
Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.
Alternative names
Paired box protein Pax-8, PAX8
Recommended products
Rabbit Recombinant Monoclonal PAX8 antibody. Carrier free. Suitable for WB, ICC/IF, IHC-Fr, IHC-P, ChIC/CUT&RUN-seq and reacts with Mouse, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR18715
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab246332 is the carrier-free version of Anti-PAX8 antibody [EPR18715] ab191870.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PAX8 also known as 3a8 is a transcription factor with a molecular mass of approximately 48 kDa. It belongs to the paired box (PAX) family of proteins which are characterized by a specific DNA-binding domain. PAX8 plays an important role in organ development and cellular differentiation. This protein is mainly expressed in the thyroid kidneys and Müllerian-derived tissues. Researchers often detect PAX8 using immunohistochemistry (PAX8 IHC) techniques due to its tissue-specific expression patterns.
- Biological function summary
The PAX8 protein regulates the expression of genes related to organogenesis and cell fate determination. It forms part of transcriptional regulatory complexes partnering with other proteins to modulate gene activity. PAX8 supports embryonic development especially in the thyroid and urogenital tract. Its presence ensures the proper function and formation of these organs highlighting its significance in developmental biology.
- Pathways
PAX8 interacts with several key biological processes. It notably participates in the thyroid hormone synthesis pathway where it regulates the expression of thyroglobulin and thyroperoxidase. PAX8 also affects the renal system through its interaction with WT1 a protein involved in kidney development. These pathways illustrate PAX8's role in maintaining endocrine and renal functions by influencing important protein interactions.
- Associated diseases and disorders
PAX8 mutations and dysregulation connect to conditions like congenital hypothyroidism and renal agenesis. In congenital hypothyroidism defects in PAX8 lead to impaired thyroid development affecting hormone production. Moreover its interaction with BCL2 an important apoptosis regulator relates to certain cancers such as ovarian carcinoma. The misregulation of PAX8 increases the risk and progression of these disorders by disrupting normal physiological processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunohistochemistry (Frozen sections) - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332), expandable thumbnail](https://content.abcam.com/products/images/pax8-antibody-epr18715-bsa-and-azide-free-ab246332--immunohistochemistry-frozen-sections-img108961.jpg "Immunohistochemistry (Frozen sections) - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)")
Immunohistochemistry (Frozen sections) - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)
Immunohistochemistry (Frozen sections) analysis of mouse kidney tissue labelling PAX8 with Anti-PAX8 antibody [EPR18715] ab191870 at a dilution of 1/500. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/1000). Nuclear staining is visible in the mouse kidney.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAX8 antibody [EPR18715] ab191870).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332), expandable thumbnail](https://content.abcam.com/products/images/pax8-antibody-epr18715-bsa-and-azide-free-ab246332--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img86315.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)
Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling PAX8 with Anti-PAX8 antibody [EPR18715] ab191870 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nucleus staining on tumor cells of thyroid carcinoma is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAX8 antibody [EPR18715] ab191870).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332), expandable thumbnail](https://content.abcam.com/products/images/pax8-antibody-epr18715-bsa-and-azide-free-ab246332--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img33484.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)
Immunohistochemical analysis of paraffin-embedded Human endometrium carcinoma tissue labeling PAX8 with Anti-PAX8 antibody [EPR18715] ab191870 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nucleus staining on tumor cells of the endometrium carcinoma is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAX8 antibody [EPR18715] ab191870).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332), expandable thumbnail](https://content.abcam.com/products/images/pax8-antibody-epr18715-bsa-and-azide-free-ab246332--immunocytochemistry-immunofluorescence-img67810.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)")
Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH:OVCAR-3 (Human ovary adenocarcinoma cell line) cells labeling PAX8 with Anti-PAX8 antibody [EPR18715] ab191870 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nucleus staining on NIH:OVCAR-3 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: Anti-PAX8 antibody [EPR18715] ab191870 at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAX8 antibody [EPR18715] ab191870).
![Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332), expandable thumbnail](https://content.abcam.com/products/images/pax8-antibody-epr18715-bsa-and-azide-free-ab246332--immunocytochemistry-immunofluorescence-img68332.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)")
Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-OV-3 (Human ovarian cancer cell line) cells labeling PAX8 with Anti-PAX8 antibody [EPR18715] ab191870 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nucleus staining on SK-OV-3 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: Anti-PAX8 antibody [EPR18715] ab191870 at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAX8 antibody [EPR18715] ab191870).
![ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332), expandable thumbnail](https://content.abcam.com/products/images/pax8-antibody-epr18715-bsa-and-azide-free-ab246332--chic-cut-run-sequencing-img424399.jpg "ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)")
ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)
"This data was developed using the same antibody clone in a different buffer formulation (Anti-PAX8 antibody [EPR18715] ab191870).
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) cells and 5 µg of Anti-PAX8 antibody [EPR18715] ab191870 [EPR18715]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods."![ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332), expandable thumbnail](https://content.abcam.com/products/images/pax8-antibody-epr18715-bsa-and-azide-free-ab246332--chic-cut-run-sequencing-img424400.jpg "ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)")
ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)
"This data was developed using the same antibody clone in a different buffer formulation (Anti-PAX8 antibody [EPR18715] ab191870).
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) cells and 5 µg of Anti-PAX8 antibody [EPR18715] ab191870 [EPR18715]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods."![ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332), expandable thumbnail](https://content.abcam.com/products/images/pax8-antibody-epr18715-bsa-and-azide-free-ab246332--chic-cut-run-sequencing-img424398.jpg "ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)")
ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)
"This data was developed using the same antibody clone in a different buffer formulation (Anti-PAX8 antibody [EPR18715] ab191870).
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) cells and 5 µg of Anti-PAX8 antibody [EPR18715] ab191870 [EPR18715]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods."
![ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332), expandable thumbnail](https://content.abcam.com/products/images/pax8-antibody-epr18715-bsa-and-azide-free-ab246332--chic-cut-run-sequencing-img424399.jpg/_jcr_content/renditions/webp-475.webp "ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)")
![ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332), expandable thumbnail](https://content.abcam.com/products/images/pax8-antibody-epr18715-bsa-and-azide-free-ab246332--chic-cut-run-sequencing-img424400.jpg/_jcr_content/renditions/webp-475.webp "ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)")
![ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332), expandable thumbnail](https://content.abcam.com/products/images/pax8-antibody-epr18715-bsa-and-azide-free-ab246332--chic-cut-run-sequencing-img424398.jpg/_jcr_content/renditions/webp-475.webp "ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [EPR18715] - BSA and Azide free (ab246332)")
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