Anti-PAX8 antibody [PAX8R1]
3
(6 Reviews)
|
(40 Publications)
Anti-PAX8 antibody [PAX8R1] (ab53490) is a mouse monoclonal antibody detecting PAX8 in Western Blot, Flow Cytometry, IP, IHC-P. Suitable for Human, Mouse, Rat.
- Over 30 publications
- Trusted since 2007
View Alternative Names
Paired box protein Pax-8, PAX8
- WB
Unknown
Western blot - Anti-PAX8 antibody [PAX8R1] (AB53490)
All lanes:
Western blot - Anti-PAX8 antibody [PAX8R1] (ab53490) at 1/100 dilution
All lanes:
HeLa whole cell lysate at 50 µg
Secondary
All lanes:
Mouse IgG antibody at 1/2500 dilution
Predicted band size: 48 kDa
Observed band size: 60 kDa
false
- ICC
Unknown
Immunocytochemistry - Anti-PAX8 antibody [PAX8R1] (AB53490)
ab53490, at a 1/10 dilution, staining PAX8 in HeLa cells, using Immunocytochemistry, plus AlexaFluor®488 Goat Anti-mouse IgG secondary anitbody.
- Flow Cyt
Unknown
Flow Cytometry - Anti-PAX8 antibody [PAX8R1] (AB53490)
HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with ab53490 at 0.5 μg/sample (shaded) or isotype control (unshaded) followed by Alexa Fluor® 488-conjugated goat anti-mouse IgG (0.25μg)
- IP
Unknown
Immunoprecipitation - Anti-PAX8 antibody [PAX8R1] (AB53490)
Immunoprecipitation : ab53490 : 5µg, RIPA lysate of HeLa cells : 500µg. WB : ab53490 at a 1 : 100 dilution, Secondary antibody - anti-mouse IgG at a 1 : 1000 dilution
All lanes:
Immunoprecipitation - Anti-PAX8 antibody [PAX8R1] (ab53490)
Predicted band size: 48 kDa
false
- WB
Unknown
Western blot - Anti-PAX8 antibody [PAX8R1] (AB53490)
All lanes:
Western blot - Anti-PAX8 antibody [PAX8R1] (ab53490) at 1/100 dilution
All lanes:
F2408 whole cell lysate at 25 µg
Secondary
All lanes:
Mouse IgG antibody at 1/2500 dilution
Predicted band size: 48 kDa
Observed band size: 60 kDa
false
- WB
Unknown
Western blot - Anti-PAX8 antibody [PAX8R1] (AB53490)
All lanes:
Western blot - Anti-PAX8 antibody [PAX8R1] (ab53490) at 1/100 dilution
All lanes:
NIH3T3 whole cell lysate at 25 µg
Secondary
All lanes:
Mouse IgG antibody at 1/2500 dilution
Predicted band size: 48 kDa
Observed band size: 60 kDa
false
Reactivity data
Product details
Anti-PAX8 antibody [PAX8R1] (ab53490) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.
What is the molecular weight of PAX8?
Anti-PAX8 [PAX8R1] (ab53490) specifically detects a band for PAX8 (UniProt: Q06710) at a molecular weight of 48kDa.
Trusted by the scientific community
Anti-PAX8 [PAX8R1] (ab53490) was first used in a scientific publication in 2007 and has been cited over 30 times in peer-reviewed journals.
Reviewed by scientists
Anti-PAX8 [PAX8R1] (ab53490) has over 5 independent reviews from customers.
Properties and storage information
Form
Purification technique
Purification notes
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The PAX8 protein regulates the expression of genes related to organogenesis and cell fate determination. It forms part of transcriptional regulatory complexes partnering with other proteins to modulate gene activity. PAX8 supports embryonic development especially in the thyroid and urogenital tract. Its presence ensures the proper function and formation of these organs highlighting its significance in developmental biology.
Pathways
PAX8 interacts with several key biological processes. It notably participates in the thyroid hormone synthesis pathway where it regulates the expression of thyroglobulin and thyroperoxidase. PAX8 also affects the renal system through its interaction with WT1 a protein involved in kidney development. These pathways illustrate PAX8's role in maintaining endocrine and renal functions by influencing important protein interactions.
Product protocols
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Target data
Publications (40)
Recent publications for all applications. Explore the full list and refine your search
NPJ precision oncology 9:306 PubMed40885787
2025
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BMC cancer 25:989 PubMed40461968
2025
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Cell death & disease 16:312 PubMed40246814
2025
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Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2305913 PubMed38059822
2023
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Molecular carcinogenesis 62:1417-1427 PubMed37265438
2023
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British journal of cancer 128:1765-1776 PubMed36810910
2023
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BMC cancer 23:44 PubMed36639622
2023
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Nature communications 13:7860 PubMed36543770
2022
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Scientific reports 12:19921 PubMed36402859
2022
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International journal of molecular sciences 23: PubMed35682896
2022
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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