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AB53490

Anti-PAX8 antibody [PAX8R1]

3

(6 Reviews)

|

(40 Publications)

Anti-PAX8 antibody [PAX8R1] (ab53490) is a mouse monoclonal antibody detecting PAX8 in Western Blot, Flow Cytometry, IP, IHC-P. Suitable for Human, Mouse, Rat.

- Over 30 publications
- Trusted since 2007

View Alternative Names

Paired box protein Pax-8, PAX8

6 Images
Western blot - Anti-PAX8 antibody [PAX8R1] (AB53490)
  • WB

Unknown

Western blot - Anti-PAX8 antibody [PAX8R1] (AB53490)

All lanes:

Western blot - Anti-PAX8 antibody [PAX8R1] (ab53490) at 1/100 dilution

All lanes:

HeLa whole cell lysate at 50 µg

Secondary

All lanes:

Mouse IgG antibody at 1/2500 dilution

Predicted band size: 48 kDa

Observed band size: 60 kDa

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Immunocytochemistry - Anti-PAX8 antibody [PAX8R1] (AB53490)
  • ICC

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Immunocytochemistry - Anti-PAX8 antibody [PAX8R1] (AB53490)

ab53490, at a 1/10 dilution, staining PAX8 in HeLa cells, using Immunocytochemistry, plus AlexaFluor®488 Goat Anti-mouse IgG secondary anitbody.

Flow Cytometry - Anti-PAX8 antibody [PAX8R1] (AB53490)
  • Flow Cyt

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Flow Cytometry - Anti-PAX8 antibody [PAX8R1] (AB53490)

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with ab53490 at 0.5 μg/sample (shaded) or isotype control (unshaded) followed by Alexa Fluor® 488-conjugated goat anti-mouse IgG (0.25μg)

Immunoprecipitation - Anti-PAX8 antibody [PAX8R1] (AB53490)
  • IP

Unknown

Immunoprecipitation - Anti-PAX8 antibody [PAX8R1] (AB53490)

Immunoprecipitation : ab53490 : 5µg, RIPA lysate of HeLa cells : 500µg. WB : ab53490 at a 1 : 100 dilution, Secondary antibody - anti-mouse IgG at a 1 : 1000 dilution

All lanes:

Immunoprecipitation - Anti-PAX8 antibody [PAX8R1] (ab53490)

Predicted band size: 48 kDa

false

Western blot - Anti-PAX8 antibody [PAX8R1] (AB53490)
  • WB

Unknown

Western blot - Anti-PAX8 antibody [PAX8R1] (AB53490)

All lanes:

Western blot - Anti-PAX8 antibody [PAX8R1] (ab53490) at 1/100 dilution

All lanes:

F2408 whole cell lysate at 25 µg

Secondary

All lanes:

Mouse IgG antibody at 1/2500 dilution

Predicted band size: 48 kDa

Observed band size: 60 kDa

false

Western blot - Anti-PAX8 antibody [PAX8R1] (AB53490)
  • WB

Unknown

Western blot - Anti-PAX8 antibody [PAX8R1] (AB53490)

All lanes:

Western blot - Anti-PAX8 antibody [PAX8R1] (ab53490) at 1/100 dilution

All lanes:

NIH3T3 whole cell lysate at 25 µg

Secondary

All lanes:

Mouse IgG antibody at 1/2500 dilution

Predicted band size: 48 kDa

Observed band size: 60 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

PAX8R1

Isotype

IgG1

Carrier free

No

Reacts with

Rat, Human, Mouse

Applications

Flow Cyt, IP, ICC, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PAX8. The exact immunogen used to generate this antibody is proprietary information.

Q06710

Reactivity data

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Product details

What is this antibody validated in?
Anti-PAX8 antibody [PAX8R1] (ab53490) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.

What is the molecular weight of PAX8?
Anti-PAX8 [PAX8R1] (ab53490) specifically detects a band for PAX8 (UniProt: Q06710) at a molecular weight of 48kDa.

Trusted by the scientific community
Anti-PAX8 [PAX8R1] (ab53490) was first used in a scientific publication in 2007 and has been cited over 30 times in peer-reviewed journals.

Reviewed by scientists
Anti-PAX8 [PAX8R1] (ab53490) has over 5 independent reviews from customers.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from TCS
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PAX8 also known as 3a8 is a transcription factor with a molecular mass of approximately 48 kDa. It belongs to the paired box (PAX) family of proteins which are characterized by a specific DNA-binding domain. PAX8 plays an important role in organ development and cellular differentiation. This protein is mainly expressed in the thyroid kidneys and Mьllerian-derived tissues. Researchers often detect PAX8 using immunohistochemistry (PAX8 IHC) techniques due to its tissue-specific expression patterns.
Biological function summary

The PAX8 protein regulates the expression of genes related to organogenesis and cell fate determination. It forms part of transcriptional regulatory complexes partnering with other proteins to modulate gene activity. PAX8 supports embryonic development especially in the thyroid and urogenital tract. Its presence ensures the proper function and formation of these organs highlighting its significance in developmental biology.

Pathways

PAX8 interacts with several key biological processes. It notably participates in the thyroid hormone synthesis pathway where it regulates the expression of thyroglobulin and thyroperoxidase. PAX8 also affects the renal system through its interaction with WT1 a protein involved in kidney development. These pathways illustrate PAX8's role in maintaining endocrine and renal functions by influencing important protein interactions.

PAX8 mutations and dysregulation connect to conditions like congenital hypothyroidism and renal agenesis. In congenital hypothyroidism defects in PAX8 lead to impaired thyroid development affecting hormone production. Moreover its interaction with BCL2 an important apoptosis regulator relates to certain cancers such as ovarian carcinoma. The misregulation of PAX8 increases the risk and progression of these disorders by disrupting normal physiological processes.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.
See full target information PAX8

Publications (40)

Recent publications for all applications. Explore the full list and refine your search

NPJ precision oncology 9:306 PubMed40885787

2025

Ex vivo 3D micro-tumour testing platform for predicting clinical response to platinum-based therapy in patients with high-grade serous ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Esmee Koedoot,Dieudonné J van der Meer,Anne M van Altena,Lieke J Ceton,Timothy J P Sijsenaar,Marta G Montero,Fanny Grillet,Jurgen M J Piek,Ruud L M Bekkers,Maurice J D L van der Vorst,Auke M T Huijben,Astrid Baalbergen,Kevin G J A Voogdt,Loes Verhoeven,Huberdina P M Smedts,Klaus Weber,Hans Marten Hazelbag,Tjalling Bosse,Els L van Persijn-van Meerten,Cornelis D de Kroon,Leo Price,Willemijn Vader,Judith R Kroep,Nelleke P B Ottevanger

BMC cancer 25:989 PubMed40461968

2025

CEBPB promotes transformation of endometrial complex atypical hyperplasia to endometrial cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahong Tan,Lin Zhao,Daoqi Wang,Xiaodie Wu,Yongxiang Bi,Hanying Wang,Na Ma,Dehong Yang,Wei Dong,Jie Zhang

Cell death & disease 16:312 PubMed40246814

2025

USP9X integrates TGF-β and hypoxia signalings to promote ovarian cancer chemoresistance via HIF-2α-maintained stemness.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenlei Zhang,Xiujie Yu,Liqi Wen,Jia'nan Wang,Zhufeng Li,Yu Zhang,Jiayu Cheng,Ronglin Kan,Wanting Zhang,Yan Shen,Shukai Yuan,Li Zhao

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2305913 PubMed38059822

2023

Regeneration of Thyroid Glands in the Spleen Restores Homeostasis in Thyroidectomy Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xue-Jiao Tian,Zhi-Jie Yin,Zhen-Jiang Li,Zhen-Zhen Wang,Zhen Xing,Chun-Yan Liu,Lin-Tao Wang,Chun-Ming Wang,Jun-Feng Zhang,Lei Dong

Molecular carcinogenesis 62:1417-1427 PubMed37265438

2023

Insulin-like growth factor (IGF) and hepatocyte growth factor (HGF) in follicular fluid cooperatively promote the oncogenesis of high-grade serous carcinoma from fallopian tube epithelial cells: Dissection of the molecular effects.

Applications

Unspecified application

Species

Unspecified reactive species

Tang-Yuan Chu,Aye Aye Khine,Na-Yi Yuan Wu,Pao-Chu Chen,Sung-Chao Chu,Ming-Hsun Lee,Hsuan-Shun Huang

British journal of cancer 128:1765-1776 PubMed36810910

2023

The DNA damage response in advanced ovarian cancer: functional analysis combined with machine learning identifies signatures that correlate with chemotherapy sensitivity and patient outcome.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas D J Walker,Zahra F Faraahi,Marcus J Price,Amy Hawarden,Caitlin A Waddell,Bryn Russell,Dominique M Jones,Aiste McCormick,N Gavrielides,S Tyagi,Laura C Woodhouse,Bethany Whalley,Connor Roberts,Emma J Crosbie,Richard J Edmondson

BMC cancer 23:44 PubMed36639622

2023

Claudin18.2 as a potential therapeutic target for primary ovarian mucinous carcinomas and metastatic ovarian mucinous carcinomas from upper gastrointestinal primary tumours.

Applications

Unspecified application

Species

Unspecified reactive species

Fujun Wang,Yao Yang,Xiuzhen Du,Xiaoying Zhu,Yanjiao Hu,Changyu Lu,Lei Sui,Han Zhao,Kejuan Song,Qin Yao

Nature communications 13:7860 PubMed36543770

2022

SOX17-positive rete testis epithelium is required for Sertoli valve formation and normal spermiogenesis in the male mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Aya Uchida,Kenya Imaimatsu,Honoka Suzuki,Xiao Han,Hiroki Ushioda,Mami Uemura,Kasane Imura-Kishi,Ryuji Hiramatsu,Hinako M Takase,Yoshikazu Hirate,Atsuo Ogura,Masami Kanai-Azuma,Akihiko Kudo,Yoshiakira Kanai

Scientific reports 12:19921 PubMed36402859

2022

Hnf1b renal expression directed by a distal enhancer responsive to Pax8.

Applications

Unspecified application

Species

Unspecified reactive species

L Goea,I Buisson,V Bello,A Eschstruth,M Paces-Fessy,R Le Bouffant,A Chesneau,S Cereghini,J F Riou,M Umbhauer

International journal of molecular sciences 23: PubMed35682896

2022

Ovulation Enhances Intraperitoneal and Ovarian Seedings of High-Grade Serous Carcinoma Cells Originating from the Fallopian Tube: Confirmation in a Bursa-Free Mouse Xenograft Model.

Applications

Unspecified application

Species

Unspecified reactive species

Che-Fang Hsu,Vaishnavi Seenan,Liang-Yuan Wang,Tang-Yuan Chu
View all publications

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