Name: Anti-PAX8 antibody [SP348] - BSA and Azide free
SKU: ab242429
Rating: 5 (1 reviews)

Anti-PAX8 [SP348] antibody (ab242429) is a rabbit monoclonal antibody that is provided in a PBS only formulation free from BSA and azide. Formulated to be conjugation-ready. It is used to detect PAX8 in Western Blot, Flow Cytometry, IHC-P, ICC/IF, mIHC. Suitable for Human samples.

- BSA, sodium azide, and glycerol-free for consistent conjugation with fluorochromes, enzymes and more

Images

Flow Cytometry - Anti-PAX8 antibody [SP348] - BSA and Azide free (AB242429), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	mIHC	Flow Cyt	WB	IHC-P	ICC/IF	ChIC/CUT&RUN-seq
Human	Tested	Tested	Expected	Tested	Tested	Tested
Mouse	Predicted	Predicted	Predicted	Predicted	Predicted	Predicted
Rat	Predicted	Predicted	Predicted	Predicted	Predicted	Predicted
Dog	Predicted	Predicted	Predicted	Predicted	Predicted	Predicted

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat, Dog	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Primary antibody incubation for 30 minutes at 4°C.

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat, Dog	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat, Dog	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Primary antibody incubation for 10 minutes at room temperature. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat, Dog	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat, Dog	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat, Dog	Dilution info -	Notes -

Associated Products

Select an associated product type

7 products for Alternative Product

1 product for Alternative Version

Target data

See full target information PAX8

Function

Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.

Alternative names

Paired box protein Pax-8, PAX8

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: SP348
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

What is this antibody validated in?

Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), Multiplex IHC (mIHC) in Human samples.

What is the molecular weight of PAX8?

Anti-PAX8 [SP348] - BSA and Azide free (ab242429) specifically detects a band for PAX8 (UniProt: Q06710) at a molecular weight of 48kDa.

Collaboration

Anti-PAX8 [SP348] - BSA and Azide free (ab242429) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).

Other related products

We have a range of other formats of antibody clone [SP348] also available for your convenience:
ab227707, Carrier free - ab242429, Alexa Fluor® 647 - ab313345

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PAX8 also known as 3a8 is a transcription factor with a molecular mass of approximately 48 kDa. It belongs to the paired box (PAX) family of proteins which are characterized by a specific DNA-binding domain. PAX8 plays an important role in organ development and cellular differentiation. This protein is mainly expressed in the thyroid kidneys and Mьllerian-derived tissues. Researchers often detect PAX8 using immunohistochemistry (PAX8 IHC) techniques due to its tissue-specific expression patterns.

Biological function summary

The PAX8 protein regulates the expression of genes related to organogenesis and cell fate determination. It forms part of transcriptional regulatory complexes partnering with other proteins to modulate gene activity. PAX8 supports embryonic development especially in the thyroid and urogenital tract. Its presence ensures the proper function and formation of these organs highlighting its significance in developmental biology.

Pathways

PAX8 interacts with several key biological processes. It notably participates in the thyroid hormone synthesis pathway where it regulates the expression of thyroglobulin and thyroperoxidase. PAX8 also affects the renal system through its interaction with WT1 a protein involved in kidney development. These pathways illustrate PAX8's role in maintaining endocrine and renal functions by influencing important protein interactions.

Associated diseases and disorders

PAX8 mutations and dysregulation connect to conditions like congenital hypothyroidism and renal agenesis. In congenital hypothyroidism defects in PAX8 lead to impaired thyroid development affecting hormone production. Moreover its interaction with BCL2 an important apoptosis regulator relates to certain cancers such as ovarian carcinoma. The misregulation of PAX8 increases the risk and progression of these disorders by disrupting normal physiological processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

15 product images

Flow Cytometry - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)
Flow Cytometry analysis of SK-OV-3 (human ovarian cancer epithelial cell) cells labeling PAX8 with purified Anti-PAX8 antibody [SP348] - N-terminal ab227707 at 1:400 dilution (1.015 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) - Black. Unlabeled control - Blue.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242429)
Flow Cytometry - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)
Flow Cytometry analysis of HL-60 (human promyelocytic leukemia cell line) cells, labeling PAX8 with Anti-PAX8 antibody [SP348] - N-terminal ab227707 at 1/100 dilution (green) compared to a Rabbit IgG negative control (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-PAX8 antibody [SP348] - N-terminal ab227707).
Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)
Immunocytochemistry/ Immunofluorescence analysis of SK-OV-3 (human ovarian cancer epithelial cell) cells labeling PAX8 with purified Anti-PAX8 antibody [SP348] - N-terminal ab227707 at 1:50 (8.1 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242429)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)
Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for PAX8 using Anti-PAX8 antibody [SP348] - N-terminal ab227707 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-PAX8 antibody [SP348] - N-terminal ab227707).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)
Formalin-fixed, paraffin-embedded human kidney tissue stained for PAX8 using Anti-PAX8 antibody [SP348] - N-terminal ab227707 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-PAX8 antibody [SP348] - N-terminal ab227707).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)
Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for PAX8 using Anti-PAX8 antibody [SP348] - N-terminal ab227707 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-PAX8 antibody [SP348] - N-terminal ab227707).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)
Formalin-fixed, paraffin-embedded human uterus tissue stained for PAX8 using Anti-PAX8 antibody [SP348] - N-terminal ab227707 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-PAX8 antibody [SP348] - N-terminal ab227707).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)
Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for PAX8 using Anti-PAX8 antibody [SP348] - N-terminal ab227707 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-PAX8 antibody [SP348] - N-terminal ab227707).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)
Formalin-fixed, paraffin-embedded human thyroid tissue stained for PAX8 using Anti-PAX8 antibody [SP348] - N-terminal ab227707 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-PAX8 antibody [SP348] - N-terminal ab227707).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)
Formalin-fixed, paraffin-embedded human fallopian tube tissue stained for PAX8 using Anti-PAX8 antibody [SP348] - N-terminal ab227707 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-PAX8 antibody [SP348] - N-terminal ab227707).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for PAX8 using Anti-PAX8 antibody [SP348] - N-terminal ab227707 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-PAX8 antibody [SP348] - N-terminal ab227707).
Multiplex immunohistochemistry - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)
Fluorescence multiplex immunohistochemical analysis of Human thyroid gland tissue (Formalin/PFA-fixed paraffin-embedded sections).

Panel A: merged staining of anti-Thyroglobulin (Anti-Thyroglobulin antibody [EPR3614(2)] ab151539, magenta; Opal™690), anti-Thyroid Peroxidase/TPO (Anti-Thyroid Peroxidase/TPO antibody [EPR5380] ab109383, green; Opal™520) and anti-PAX8 (Anti-PAX8 antibody [SP348] - N-terminal ab227707, red; Opal™570) on human thyroid gland. Panel B: anti-Thyroid Peroxidase/TPO stained on cytoplasm of follicular epithelial cells. Panel C: anti-PAX8 stained on nucleus of follicular epithelial cells. Panel D: anti-Thyroglobulin stained on colloid. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

The section was incubated in three rounds of staining: in the order of Anti-Thyroglobulin antibody [EPR3614(2)] ab151539 at 1/5000 dilution (0.167 μg/ml), Anti-Thyroid Peroxidase/TPO antibody [EPR5380] ab109383 at 1/1500 dilution (0.072 μg/ml) , and Anti-PAX8 antibody [SP348] - N-terminal ab227707 at 1/200 dilution (5.22 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. DAPI (blue) was used as a nuclear counter stain.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-PAX8 antibody [SP348] - N-terminal ab227707).
ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)
"This data was developed using the same antibody clone in a different buffer formulation (Anti-PAX8 antibody [SP348] - N-terminal ab227707).
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) cells and 5 µg of Anti-PAX8 antibody [SP348] - N-terminal ab227707 [SP348]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods."
ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)
"This data was developed using the same antibody clone in a different buffer formulation (Anti-PAX8 antibody [SP348] - N-terminal ab227707).
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) cells and 5 µg of Anti-PAX8 antibody [SP348] - N-terminal ab227707 [SP348]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods."
ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [SP348] - BSA and Azide free (ab242429)
"This data was developed using the same antibody clone in a different buffer formulation (Anti-PAX8 antibody [SP348] - N-terminal ab227707).
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) cells and 5 µg of Anti-PAX8 antibody [SP348] - N-terminal ab227707 [SP348]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods."