Name: Anti-PAX8 antibody [SP348] - N-terminal
SKU: ab227707
Rating: 4 (1 reviews)

Anti-PAX8 antibody [SP348] - N-terminal is a rabbit recombinant monoclonal antibody that is used to detect PAX8 in ChIC/CUT&RUN-seq, Flow cytometry, ICC/IF, IHC-P, Western blot, mIHC. Suitable for Human samples.

- Recombinant format for unrivaled batch-batch consistency

Images

Multiplex immunohistochemistry - Anti-PAX8 antibody [SP348] - N-terminal (AB227707), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.1% Sodium azide
Constituents: 98.9% PBS, 1% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	mIHC	Flow Cyt	WB	IHC-P	ICC/IF	ChIC/CUT&RUN-seq
Human	Tested	Tested	Tested	Tested	Tested	Tested
Mouse	Predicted	Predicted	Predicted	Predicted	Predicted	Predicted
Rat	Predicted	Predicted	Predicted	Predicted	Predicted	Predicted
Dog	Predicted	Predicted	Predicted	Predicted	Predicted	Predicted

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/200	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat, Dog	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes Primary antibody incubation for 30 minutes at 4°C.

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat, Dog	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes Primary antibody incubation for 1 hour at room temperature.

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat, Dog	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol. Primary antibody incubation for 10 minutes at room temperature.

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat, Dog	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat, Dog	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat, Dog	Dilution info -	Notes -

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

See full target information PAX8

Function

Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.

Alternative names

Paired box protein Pax-8, PAX8

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.1% Sodium azide
Constituents: 98.9% PBS, 1% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: SP348
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Anti-PAX8 antibody [SP348] - N-terminal (ab227707) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ChIC/CUT&RUN-seq, Flow Cyt ICC/IF, IHC-P, WB and mIHC.

Abcam's high quality manufacturing and validation processes ensure Anti-PAX8 antibody [SP348] - N-terminal (ab227707) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Anti-PAX8 antibody [SP348] - N-terminal (ab227707) specifically detects PAX8 (UniProt ID: Q06710; Molecular weight: 49kDa) and is sold in 100 µL, 500 µL and 1 mL selling sizes.

Conjugation-ready, carrier free format available for antibody clone SP348 - Anti-PAX8 antibody [SP348] - BSA and Azide free ab242429.

Antibody clone SP348 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor^® 647 (Alexa Fluor® 647 Anti-PAX8 - N-terminal antibody [SP348] ab313345).

Anti-PAX8 antibody [SP348] - N-terminal (ab227707) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).

Highly-specific to PAX8. Western blotting data to guarantee specificity to PAX8 with no cross-reactivity with other PAX family members. Validated for CUT&RUN-seq, a key application to map protein-DNA interactions on a genome-wide scale using NGS. A highly-validated antibody for studying PAX8 as a transcription factor, its role in thyroid and renal cancers and epigenetic regulation. This antibody is crucial in cancer research, particularly in understanding PAX8's involvement in tumorigenesis and gene expression and is widely used in studies of embryogenesis and cancers of the Mьllerian duct.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PAX8 also known as 3a8 is a transcription factor with a molecular mass of approximately 48 kDa. It belongs to the paired box (PAX) family of proteins which are characterized by a specific DNA-binding domain. PAX8 plays an important role in organ development and cellular differentiation. This protein is mainly expressed in the thyroid kidneys and Müllerian-derived tissues. Researchers often detect PAX8 using immunohistochemistry (PAX8 IHC) techniques due to its tissue-specific expression patterns.

Biological function summary

The PAX8 protein regulates the expression of genes related to organogenesis and cell fate determination. It forms part of transcriptional regulatory complexes partnering with other proteins to modulate gene activity. PAX8 supports embryonic development especially in the thyroid and urogenital tract. Its presence ensures the proper function and formation of these organs highlighting its significance in developmental biology.

Pathways

PAX8 interacts with several key biological processes. It notably participates in the thyroid hormone synthesis pathway where it regulates the expression of thyroglobulin and thyroperoxidase. PAX8 also affects the renal system through its interaction with WT1 a protein involved in kidney development. These pathways illustrate PAX8's role in maintaining endocrine and renal functions by influencing important protein interactions.

Associated diseases and disorders

PAX8 mutations and dysregulation connect to conditions like congenital hypothyroidism and renal agenesis. In congenital hypothyroidism defects in PAX8 lead to impaired thyroid development affecting hormone production. Moreover its interaction with BCL2 an important apoptosis regulator relates to certain cancers such as ovarian carcinoma. The misregulation of PAX8 increases the risk and progression of these disorders by disrupting normal physiological processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

17 product images

Multiplex immunohistochemistry - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
Fluorescence multiplex immunohistochemical analysis of Human thyroid gland tissue (Formalin/PFA-fixed paraffin-embedded sections).

Panel A: merged staining of anti-Thyroglobulin (Anti-Thyroglobulin antibody [EPR3614(2)] ab151539, magenta; Opal™690), anti-Thyroid Peroxidase/TPO (Anti-Thyroid Peroxidase/TPO antibody [EPR5380] ab109383, green; Opal™520) and anti-PAX8 (ab227707, red; Opal™570) on human thyroid gland. Panel B: anti-Thyroid Peroxidase/TPO stained on cytoplasm of follicular epithelial cells. Panel C: anti-PAX8 stained on nucleus of follicular epithelial cells. Panel D: anti-Thyroglobulin stained on colloid. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

The section was incubated in three rounds of staining: in the order of Anti-Thyroglobulin antibody [EPR3614(2)] ab151539 at 1/5000 dilution (0.167 μg/ml), Anti-Thyroid Peroxidase/TPO antibody [EPR5380] ab109383 at 1/1500 dilution (0.072 μg/ml) , and ab227707 at 1/200 dilution (5.22 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. DAPI (blue) was used as a nuclear counter stain.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Flow Cytometry - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
Flow Cytometry analysis of SK-OV-3 (human ovarian cancer epithelial cell) cells labeling PAX8 with purified ab227707 at 1:400 dilution (1.015 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) - Black. Unlabeled control - Blue.
Flow Cytometry - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
Flow Cytometry analysis of HL-60 (human promyelocytic leukemia cell line) cells, labeling PAX8 with ab227707 at 1/100 dilution (green) compared to a Rabbit IgG negative control (blue).
Immunocytochemistry/ Immunofluorescence - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
Immunocytochemistry/ Immunofluorescence analysis of SK-OV-3 (human ovarian cancer epithelial cell) cells labeling PAX8 with purified ab227707 at 1:50 (8.1 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Western blot - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
All lanes: Western blot - Anti-PAX8 antibody [SP348] - N-terminal (ab227707) at 0.398 µg/mL
Lane 1: SK-OV-3 (human ovarian cancer epithelial cell) cell lysate at 20 µg
Lane 2: human kidney tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 0.05 µg/mL
Predicted band size: 48 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.
Western blot - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
All lanes: Western blot - Anti-PAX8 antibody [SP348] - N-terminal (ab227707) at 1/100 dilution
Lane 1: PAX1 transfected HEK-293 (human epithelial cel line from embryonic kidney) cell lysate
Lane 2: PAX2 transfected HEK-293 (human epithelial cel line from embryonic kidney) cell lysate
Lane 3: PAX3 transfected HEK-293 (human epithelial cel line from embryonic kidney) cell lysate
Lane 4: PAX4 transfected HEK-293 (human epithelial cel line from embryonic kidney) cell lysate
Lane 5: PAX5 transfected HEK-293 (human epithelial cel line from embryonic kidney) cell lysate
Lane 6: PAX6 transfected HEK-293 (human epithelial cel line from embryonic kidney) cell lysate
Lane 7: PAX7 transfected HEK-293 (human epithelial cel line from embryonic kidney) cell lysate
Lane 8: PAX8 transfected HEK-293 (human epithelial cel line from embryonic kidney) cell lysate
Lane 9: PAX9 transfected HEK-293 (human epithelial cel line from embryonic kidney) cell lysate
Lane 10: Mock transfected HEK-293 (human epithelial cel line from embryonic kidney) cell lysate
Predicted band size: 48 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
Formalin-fixed, paraffin-embedded human kidney tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
Formalin-fixed, paraffin-embedded human uterus tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
Formalin-fixed, paraffin-embedded human thyroid tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
Formalin-fixed, paraffin-embedded human fallopian tube tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for PAX8 using ab227707 at 1/100 dilution in immunohistochemical analysis.
ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) cells and 5 µg of ab227707 [SP348]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods."
ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) cells and 5 µg of ab227707 [SP348]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods."
ChIC/CUT&RUN sequencing - Anti-PAX8 antibody [SP348] - N-terminal (ab227707)
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) cells and 5 µg of ab227707 [SP348]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods."