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AB28538

Anti-PAX9 antibody [7C2]

4

(1 Review)

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(11 Publications)

Rat Recombinant Monoclonal PAX9 antibody. Suitable for ICC/IF, WB and reacts with Mouse samples. Cited in 11 publications.

View Alternative Names

Pax-9, Paired box protein Pax-9

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-PAX9 antibody [7C2] (AB28538)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-PAX9 antibody [7C2] (AB28538)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling PAX9 with ab28538 at 1/20 (49.7 μg/ml) dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strong nuclear staining in NIH/3T3 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Western blot - Anti-PAX9 antibody [7C2] (AB28538)
  • WB

Lab

Western blot - Anti-PAX9 antibody [7C2] (AB28538)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-PAX9 antibody [7C2] (ab28538) at 1/1000 dilution

Lane 1:

Mouse esophagus tissue lysate at 20 µg

Lane 2:

Mouse skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/10000 dilution

Predicted band size: 36 kDa

false

Exposure time: 70s

  • Carrier free

    Anti-PAX9 antibody [7C2] - BSA and Azide free

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

7C2

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Mouse

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Does not cross react with other PAX proteins

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Complementary Products

Primary Antibodies

AB53715

Anti-Patched / PTCH1 antibody

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Primary Antibodies

AB155279

Anti-SOX10 antibody [EPR4007]

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Immunohistochemistry (Frozen sections) - Anti-SOX10 antibody [EPR4007] (AB155279)

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Primary Antibodies

AB97959

Anti-SOX2 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (AB97959)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/20", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

This product has switched from a hybridoma to recombinant production method on 08th March 2021.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PAX9 also known as paired box gene 9 is a transcription factor with a molecular mass of approximately 37 kDa. It belongs to the PAX family of proteins characterized by the presence of a paired box domain. Mechanically PAX9 plays a role in DNA binding and transcriptional regulation of target genes. It is expressed in developing teeth craniofacial regions limbs and parts of the neural crest. This expression pattern highlights PAX9’s involvement in early developmental processes.
Biological function summary

PAX9 contributes significantly to developmental patterning and organogenesis. It functions as a part of a transcription factor complex that coordinates the gene networks necessary for these processes. PAX9 interacts with other PAX family members and developmental regulators mediating the formation of structures such as teeth and skeletal elements. PAX9’s role in these processes ensures proper structural differentiation and morphogenesis during development.

Pathways

PAX9 plays an important role in the tooth development pathway and the Wnt signaling pathway. During tooth development it interacts with proteins like MSX1 and BMP4 guiding tooth patterning and morphogenesis. In the Wnt pathway PAX9 influences cell signaling pivotal for cell fate determination and embryonic development where it cooperates with Wnt proteins to modulate pathway-specific gene expression.

Mutations or dysregulation of PAX9 have been associated with conditions such as tooth agenesis and oral clefts. Tooth agenesis which involves missing teeth arises from disruptions in PAX9’s functionality during tooth development often involving MSX1 as well. Oral clefts such as cleft lip and palate also involve PAX9 interactions during craniofacial development. These associations emphasize PAX9's critical role in maintaining normal developmental functions and its impact on congenital anomalies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor required for normal development of thymus, parathyroid glands, ultimobranchial bodies, teeth, skeletal elements of skull and larynx as well as distal limbs.
See full target information Pax9
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Science advances 6: PubMed33328221

2020

Spatiotemporal cellular movement and fate decisions during first pharyngeal arch morphogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Yuan,Yong-Hwee Eddie Loh,Xia Han,Jifan Feng,Thach-Vu Ho,Jinzhi He,Junjun Jing,Kimberly Groff,Alan Wu,Yang Chai

Journal of gastroenterology 55:763-774 PubMed32556644

2020

Retinoic acid receptor γ activation promotes differentiation of human induced pluripotent stem cells into esophageal epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Yasufumi Koterazawa,Michiyo Koyanagi-Aoi,Keiichiro Uehara,Yoshihiro Kakeji,Takashi Aoi

The Journal of clinical investigation 130:4396-4410 PubMed32427591

2020

Noggin regulates foregut progenitor cell programming, and misexpression leads to esophageal atresia.

Applications

Unspecified application

Species

Unspecified reactive species

Carolina Pinzon-Guzman,Sreedhara Sangadala,Katherine M Riera,Evgenya Y Popova,Elizabeth Manning,Won Jae Huh,Matthew S Alexander,Julia S Shelton,Scott D Boden,James R Goldenring

Development (Cambridge, England) 146: PubMed31444215

2019

is required for cardiovascular development and interacts with in the pharyngeal endoderm to control 4th pharyngeal arch artery morphogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Helen M Phillips,Catherine A Stothard,Wasay M Shaikh Qureshi,Anastasia I Kousa,J Alberto Briones-Leon,Ramada R Khasawneh,Chloe O'Loughlin,Rachel Sanders,Silvia Mazzotta,Rebecca Dodds,Kerstin Seidel,Timothy Bates,Mitsushiro Nakatomi,Simon J Cockell,Jürgen E Schneider,Timothy J Mohun,René Maehr,Ralf Kist,Heiko Peters,Simon D Bamforth

Cell reports 28:257-266.e5 PubMed31269445

2019

Polycomb Repressive Complex 1 Controls Maintenance of Fungiform Papillae by Repressing Sonic Hedgehog Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Carmit Bar,Idan Cohen,Dejian Zhao,Venu Pothula,Anna Litskevitch,Haruhiko Koseki,Deyou Zheng,Elena Ezhkova

Developmental cell 44:679-693.e5 PubMed29587142

2018

Developmental History Provides a Roadmap for the Emergence of Tumor Plasticity.

Applications

Unspecified application

Species

Unspecified reactive species

Purushothama Rao Tata,Ryan D Chow,Srinivas Vinod Saladi,Aleksandra Tata,Arvind Konkimalla,Anne Bara,Daniel Montoro,Lida P Hariri,Angela R Shih,Mari Mino-Kenudson,Hongmei Mou,Shioko Kimura,Leif W Ellisen,Jayaraj Rajagopal

Development (Cambridge, England) 144:2560-2569 PubMed28576771

2017

BMP signaling orchestrates a transcriptional network to control the fate of mesenchymal stem cells in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jifan Feng,Junjun Jing,Jingyuan Li,Hu Zhao,Vasu Punj,Tingwei Zhang,Jian Xu,Yang Chai

Developmental dynamics : an official publication of the American Association of Anatomists 238:29-42 PubMed19097184

2008

Transcriptional dynamics of endodermal organ formation.

Applications

ICC/IF

Species

Mouse

Richard I Sherwood,Tzong-Yang Albert Chen,Douglas A Melton

Proceedings of the National Academy of Sciences of 104:16663-8 PubMed17925434

2007

Oncogenic cooperation and coamplification of developmental transcription factor genes in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jude Kendall,Qing Liu,Amy Bakleh,Alex Krasnitz,Ken C Q Nguyen,B Lakshmi,William L Gerald,Scott Powers,David Mu

Mechanisms of development 121:1313-22 PubMed15454262

2004

Pax9 is required for filiform papilla development and suppresses skin-specific differentiation of the mammalian tongue epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Leon Jonker,Ralf Kist,Andrew Aw,Ilka Wappler,Heiko Peters
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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