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AB255977

Anti-PAX9 antibody [7C2] - BSA and Azide free

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(1 Publication)

Rat Recombinant Monoclonal PAX9 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

Paired box protein Pax-9, PAX9

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-PAX9 antibody [7C2] - BSA and Azide free (AB255977)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-PAX9 antibody [7C2] - BSA and Azide free (AB255977)

This data was developed using ab28538 the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling PAX9 with ab28538 at 1/20 (49.7 µg/ml) dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strong nuclear staining in NIH/3T3 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Western blot - Anti-PAX9 antibody [7C2] - BSA and Azide free (AB255977)
  • WB

Lab

Western blot - Anti-PAX9 antibody [7C2] - BSA and Azide free (AB255977)

This data was developed using ab28538, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 70 seconds

All lanes:

Western blot - Anti-PAX9 antibody [7C2] - BSA and Azide free (ab255977) at 1/1000 dilution

Lane 1:

Mouse esophagus tissue lysate at 20 µg

Lane 2:

Mouse skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/10000 dilution

Predicted band size: 36 kDa

false

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

7C2

Isotype

IgG1

Carrier free

Yes

Reacts with

Mouse

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Does not cross react with other PAX proteins

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB97959

Anti-SOX2 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (AB97959)

  • 4
  • 75
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB53715

Anti-Patched / PTCH1 antibody

Western blot - Anti-Patched / PTCH1 antibody (AB53715)

  • 4
  • 7
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab255977 is the carrier-free version of ab28538.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PAX9 also known as paired box gene 9 is a transcription factor with a molecular mass of approximately 37 kDa. It belongs to the PAX family of proteins characterized by the presence of a paired box domain. Mechanically PAX9 plays a role in DNA binding and transcriptional regulation of target genes. It is expressed in developing teeth craniofacial regions limbs and parts of the neural crest. This expression pattern highlights PAX9’s involvement in early developmental processes.
Biological function summary

PAX9 contributes significantly to developmental patterning and organogenesis. It functions as a part of a transcription factor complex that coordinates the gene networks necessary for these processes. PAX9 interacts with other PAX family members and developmental regulators mediating the formation of structures such as teeth and skeletal elements. PAX9’s role in these processes ensures proper structural differentiation and morphogenesis during development.

Pathways

PAX9 plays an important role in the tooth development pathway and the Wnt signaling pathway. During tooth development it interacts with proteins like MSX1 and BMP4 guiding tooth patterning and morphogenesis. In the Wnt pathway PAX9 influences cell signaling pivotal for cell fate determination and embryonic development where it cooperates with Wnt proteins to modulate pathway-specific gene expression.

Mutations or dysregulation of PAX9 have been associated with conditions such as tooth agenesis and oral clefts. Tooth agenesis which involves missing teeth arises from disruptions in PAX9’s functionality during tooth development often involving MSX1 as well. Oral clefts such as cleft lip and palate also involve PAX9 interactions during craniofacial development. These associations emphasize PAX9's critical role in maintaining normal developmental functions and its impact on congenital anomalies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor required for normal development of thymus, parathyroid glands, ultimobranchial bodies, teeth, skeletal elements of skull and larynx as well as distal limbs.
See full target information PAX9
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1040 PubMed40640354

2025

Fortilin deficiency induces anti-atherosclerotic phenotypes in macrophages and protects hypercholesterolemic mice against atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Nattaporn Wanachottrakul,Decha Pinkaew,Sandipan Mukherjee,Preedakorn Chunhacha,Mari Nakashima,Asa A Brockman,Uttariya Pal,Hasseri B Halim,Yuhong Wei,Lena Tanaka,Hanna Huynh,Kota Ramana,Shiyou Chen,Rebecca A Ihrie,Ken Fujise
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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