AB2264

Anti-Paxillin antibody

(5 Reviews)

(15 Publications)

Rabbit Polyclonal Paxillin antibody. Suitable for WB and reacts with Mouse samples. Cited in 15 publications.

View Alternative Names

Paxillin, Pxn

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Western blot - Anti-Paxillin antibody (AB2264)

ab2264 detects a band at 68 kDa in NIH 3T3 lysate, as well as an additional band at 43kDa. An NCBI BLAST search of the peptide sequence identified an N terminal fragment of the Paxillin molecule with a primary molecular weight of 36 kDa. We believe the 43 kDa band observed may correspond to this fragment.

All lanes:

Western blot - Anti-Paxillin antibody (ab2264) at 1/500 dilution

All lanes:

NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 65 kDa

Observed band size: 43 kDa,68 kDa

false

Exposure time: 20min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Paxillin also known as PXN is a cytoskeletal protein involved in the assembly of focal adhesions. It has a molecular weight of approximately 68 kDa. You will find Paxillin expressed widely in various tissues including the liver colon and lung. Paxillin plays a part in cell motility by interacting with other focal adhesion proteins. It plays a significant mechanical role by serving as a docking site for structural and signaling molecules.

Biological function summary

Paxillin serves key functions in cellular processes such as migration proliferation and survival. It interacts with different proteins including vinculin and talin forming part of a complex at the focal adhesion sites. The phosphorylation state of Paxillin can modulate its interactions and in this way influence the assembly of signaling complexes that control dynamic cellular processes.

Pathways

Paxillin participates in the integrin signaling and MAPK pathways. It operates by linking integrin receptors to the actin cytoskeleton facilitating signal transduction. Paxillin phosphorylation is an important regulatory mechanism within these pathways. In particular its interaction with focal adhesion kinase (FAK) and Src family kinases signifies its role in transmitting signals from the extracellular matrix to the cellular interior which impacts cell behavior and response.

Paxillin has links with osteosarcoma and lung cancer. Deregulation of Paxillin expression or its phosphorylation state contributes to oncogenic transformation and tumor progression. In cancer Paxillin associates with altered activity of proteins such as Src and FAK exacerbating disease states. This relationship highlights Paxillin's potential as a biomarker and therapeutic target in treating related malignancies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Recruits other proteins such as TRIM15 to focal adhesion.

See full target information Pxn

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Neuroscience 448:160-171 PubMed33002558

2020

Fmn2 Regulates Growth Cone Motility by Mediating a Molecular Clutch to Generate Traction Forces.

Applications

Unspecified application

Species

Unspecified reactive species

Ketakee Ghate,Sampada P Mutalik,Lakshmi Kavitha Sthanam,Shamik Sen,Aurnab Ghose

Oncology reports 44:1105-1115 PubMed32705241

2020

Paxillin knockdown suppresses metastasis and epithelial‑mesenchymal transition in colorectal cancer via the ERK signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Long Wen,Xiaoqian Zhang,Junling Zhang,Shanwen Chen,Yongchen Ma,Jianwen Hu,Taohua Yue,Jingui Wang,Jing Zhu,Tao Wu,Xin Wang

The Journal of clinical endocrinology and metabolism 105: PubMed32392277

2020

Mutation of PFN1 Gene in an Early Onset, Polyostotic Paget-like Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Daniela Merlotti,Maria Materozzi,Simone Bianciardi,Vito Guarnieri,Domenico Rendina,Luca Volterrani,Cristiana Bellan,Christian Mingiano,Tommaso Picchioni,Alessandro Frosali,Ugo Orfanelli,Simone Cenci,Luigi Gennari

Science signaling 12: PubMed31040262

2019

CD13 tethers the IQGAP1-ARF6-EFA6 complex to the plasma membrane to promote ARF6 activation, β1 integrin recycling, and cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Mallika Ghosh,Robin Lo,Ivan Ivic,Brian Aguilera,Veneta Qendro,Charan Devarakonda,Linda H Shapiro

Stem cells (Dayton, Ohio) 37:318-331 PubMed30512203

2019

Endothelial Differentiation G Protein-Coupled Receptor 5 Plays an Important Role in Induction and Maintenance of Pluripotency.

Applications

Unspecified application

Species

Unspecified reactive species

Irina Neganova,Lewis Cotts,Peter Banks,Katja Gassner,Anvar Shukurov,Lyle Armstrong,Graham Ladds,Majlinda Lako

Experimental and therapeutic medicine 16:1621-1628 PubMed30186380

2018

HOXD10 silencing suppresses human fibroblast-like synoviocyte migration in rheumatoid arthritis via downregulation of the p38/JNK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Luan Luan,Yingying Ma,Lihua Zhang

Biomaterials 168:1-9 PubMed29574131

2018

A tumor-activatable particle with antimetastatic potential in breast cancer via inhibiting the autophagy-dependent disassembly of focal adhesion.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Wang,Sheng Yin,Li Zhang,Kairong Shi,Jiajing Tang,Zhirong Zhang,Qin He

Experimental and therapeutic medicine 14:4595-4601 PubMed29104667

2017

Promotion of anoxia-reoxygenation-induced inflammation and permeability enhancement by nicotinamide phosphoribosyltransferase-activated MAPK signaling in human umbilical vein endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Nao Yan,Wei Yang,Xiao Dong,Qiao Fang,Yi Gong,Jian-Liang Zhou,Jian-Jun Xu

Nature communications 6:6385 PubMed25721933

2015

Semaphorin7A regulates neuroglial plasticity in the adult hypothalamic median eminence.

Applications

Species

Unspecified reactive species

Jyoti Parkash,Andrea Messina,Fanny Langlet,Irene Cimino,Anne Loyens,Danièle Mazur,Sarah Gallet,Eglantine Balland,Samuel A Malone,François Pralong,Gabriella Cagnoni,Roberta Schellino,Silvia De Marchis,Massimiliano Mazzone,R Jeroen Pasterkamp,Luca Tamagnone,Vincent Prevot,Paolo Giacobini

International journal of clinical and experimental 8:1328-40 PubMed25973017

2015

MicroRNA-145 suppresses cell migration and invasion by targeting paxillin in human colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Qin,Feiran Wang,Haiyan Jiang,Junfei Xu,Yasu Jiang,Zhiwei Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Paxillin antibody

Western blot - Anti-Paxillin antibody (AB2264)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

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