Anti-Paxillin antibody [E228]
- RabMAb
- Recombinant
- KO Validated
- What is this?
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(31 Publications)
Rabbit Recombinant Monoclonal Paxillin antibody. Suitable for IP, ELISA, Dot, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat, Synthetic peptide - Human samples. Cited in 31 publications.
View Alternative Names
Paxillin, PXN
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Paxillin antibody [E228] (AB32115)
Immunohistochemical analysis of Paxillin expression in paraffin-embedded human colon carcinoma using 1/100 ab32115.
- ICC/IF
PubMed
Immunocytochemistry/ Immunofluorescence - Anti-Paxillin antibody [E228] (AB32115)
Immunofluorescence analysis of HeLa cells, staining Paxillin with ab32115.
Cells on the right were treated with MGAT1 shRNA. Cells were fixed with 2% paraformaldehyde, permeabilized using 0.2% Triton-X-100 and blocked by 5% BSA for 1 hour. Cells were incubated with primary antibody (1/400) overnight at 4°C. A FITC-conjugated donkey anti-rabbit IgG (1/500) was used as the secondary antibody.
Image from Beheshti Zavareh R et al. PLoS One. 2012;7(9):e43721. doi: 10.1371/journal.pone.0043721. Epub 2012 Sep 5. Fig 3.; doi:10.1371/journal.pone.0043721; September 5 2012 PLoS ONE 7(9): e43721.
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-Paxillin antibody [E228] (AB32115)
Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Paxillin with Purified ab32115 at 1 : 100 dilution (1.2 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
- Flow Cyt (Intra)
Unknown
Flow Cytometry (Intracellular) - Anti-Paxillin antibody [E228] (AB32115)
Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Paxillin with Purified ab32115 at 1/20 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Paxillin antibody [E228] (AB32115)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling Paxillin with Purified ab32115 at 1 : 50 dilution (2.34 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
- IP
Unknown
Immunoprecipitation - Anti-Paxillin antibody [E228] (AB32115)
ab32115 (purified) at 1 : 20 dilution (0.5μg) immunoprecipitating Paxillin in HEK-293 whole cell lysate.
Lane 1 (input) : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate 10μg
Lane 2 (+) : ab32115 & HEK-293 whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab32115 in HEK-293 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.
All lanes:
Immunoprecipitation - Anti-Paxillin antibody [E228] (ab32115)
Predicted band size: 65 kDa
false
- ELISA
Unknown
ELISA - Anti-Paxillin antibody [E228] (AB32115)
Direct ELISA antigen dose-response curve using ab32115 at 0-1000 ng/mL. Antigen (human Paxillin phospho Y31 peptide/ unmodified peptide) concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
- WB
Unknown
Western blot - Anti-Paxillin antibody [E228] (AB32115)
Based on the immunogen sequence blast, this antibody recognizes alpha, beta and gamma isoforms. The molecular weight observed is consistent with what has been described in the literature PMID : 9712867 and 20388733
All lanes:
Western blot - Anti-Paxillin antibody [E228] (ab32115) at 1/1000 dilution
Lane 1:
HEK-293 (Human embryonic kidney) whole cell lysates at 15 µg
Lane 2:
NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 15 µg
Lane 3:
C2C12 (Mouse myoblasts myoblast) whole cell lysates at 15 µg
Lane 4:
Rat-1 (Rat embryonic fibroblast) whole cell lysates at 15 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)
Predicted band size: 65 kDa
Observed band size: 60 kDa,64 kDa
false
- WB
Lab
Western blot - Anti-Paxillin antibody [E228] (AB32115)
Lanes 1 - 4 : Merged signal (red and green). Green - ab32115 observed at 65 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32115 was shown to specifically react with PXN in wild-type A-431 cells as signal was lost in PXN knockout cell line ab261892 (knockout cell lysate ab261701). Wild-type and PXN knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. ab32115 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Paxillin antibody [E228] (ab32115) at 1/10000 dilution
Lane 1:
Wild-type A431 whole cell lysate at 20 µg
Lane 2:
PXN knockout A431 whole cell lysate at 20 µg
Lane 2:
Western blot - Human PXN (Paxillin) knockout A-431 cell line (<a href='/en-us/products/cell-lines/human-pxn-paxillin-knockout-a-431-cell-line-ab261892'>ab261892</a>)
Lane 3:
U-87 MG whole cell lysate at 20 µg
Lane 4:
PC-3 whole cell lysate at 20 µg
Predicted band size: 65 kDa
Observed band size: 65 kDa
false
Exposure time: 10s
- WB
Lab
Western blot - Anti-Paxillin antibody [E228] (AB32115)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
All lanes:
Western blot - Anti-Paxillin antibody [E228] (ab32115) at 1/1000 dilution
Lane 1:
HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Lane 2:
HEK-293 (human embryonic kidney) whole cell lysates at 15 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 64 kDa
false
Exposure time: 3min
- WB
Unknown
Western blot - Anti-Paxillin antibody [E228] (AB32115)
All lanes:
Western blot - Anti-Paxillin antibody [E228] (ab32115) at 1/10000 dilution
Lane 1:
untreated NIH 3T3 cell lysate
Lane 2:
PDGF treated NIH 3T3 cell lysate
Predicted band size: 65 kDa
Observed band size: 64 kDa
false
- Dot
Unknown
Dot Blot - Anti-Paxillin antibody [E228] (AB32115)
Dot blot analysis of Paxillin (pY31) peptide (Lane 1) and Paxillin non-phospho peptide (Lane 2) labelling Paxillin with ab32115 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.
Blocking and dilution buffer : 5% NFDM/TBST.
Exposure time : 3 minutes.
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Anti-Paxillin antibody [E228] - BSA and Azide free
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578 PE
PE Anti-Paxillin antibody [E228]
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HRP Anti-Paxillin antibody [E228]
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617 Alexa Fluor® 594
Alexa Fluor® 594 Anti-Paxillin antibody [E228]
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665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-Paxillin antibody [E228]
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565 Alexa Fluor® 555
Alexa Fluor® 555 Anti-Paxillin antibody [E228]
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603 Alexa Fluor® 568
Alexa Fluor® 568 Anti-Paxillin antibody [E228]
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660 APC
APC Anti-Paxillin antibody [E228]
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519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-Paxillin antibody [E228]
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775 Alexa Fluor® 750
Alexa Fluor® 750 Anti-Paxillin antibody [E228]
Reactivity data
Product details
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Paxillin serves key functions in cellular processes such as migration proliferation and survival. It interacts with different proteins including vinculin and talin forming part of a complex at the focal adhesion sites. The phosphorylation state of Paxillin can modulate its interactions and in this way influence the assembly of signaling complexes that control dynamic cellular processes.
Pathways
Paxillin participates in the integrin signaling and MAPK pathways. It operates by linking integrin receptors to the actin cytoskeleton facilitating signal transduction. Paxillin phosphorylation is an important regulatory mechanism within these pathways. In particular its interaction with focal adhesion kinase (FAK) and Src family kinases signifies its role in transmitting signals from the extracellular matrix to the cellular interior which impacts cell behavior and response.
Product protocols
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Target data
Publications (31)
Recent publications for all applications. Explore the full list and refine your search
Translational cancer research 13:6347-6363 PubMed39697757
2024
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Respiratory research 25:426 PubMed39633451
2024
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Nature communications 15:6509 PubMed39095354
2024
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Experimental cell research 441:114155 PubMed39002689
2024
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Communications biology 7:577 PubMed38755434
2024
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International journal of molecular sciences 24: PubMed37958964
2023
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Journal of biomedical science 30:80 PubMed37726723
2023
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Frontiers in cell and developmental biology 11:1199069 PubMed37427378
2023
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Hepatology communications 7: PubMed37314767
2023
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Nature immunology 24:1173-1187 PubMed37291385
2023
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Product promise
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