Rabbit Recombinant Monoclonal Paxillin phospho Y118 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples. Cited in 7 publications.
Images
![Western blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (AB109547), expandable thumbnail](https://content.abcam.com/products/images/paxillin-phospho-y118-antibody-epr1903-ab109547--western-blot-img20182.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (AB109547)")
![Dot Blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (AB109547), expandable thumbnail](https://content.abcam.com/products/images/paxillin-phospho-y118-antibody-epr1903-ab109547--dot-blot-img18149.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (AB109547)")
![Western blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (AB109547), expandable thumbnail](https://content.abcam.com/products/images/paxillin-phospho-y118-antibody-epr1903-ab109547--western-blot-img69574.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (AB109547)")
Publications
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- Frontiers in pharmacology 13:8556262022Resibufogenin Targets the ATP1A1 Signaling Cascade to Induce G2/M Phase Arrest and Inhibit Invasion in Glioma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXun Zhang,Zhong Yao,Zhiyi Xue,Shuai Wang,Xuemeng Liu,Yaotian Hu,Yan Zhang,Jian Wang,Xingang Li,Anjing ChenPubMed 35656311
- Phytomedicine : international journal of phytotherapy and phytopharmacology 96:1539082022Astragaloside IV promotes the angiogenic capacity of adipose-derived mesenchymal stem cells in a hindlimb ischemia model by FAK phosphorylation via CXCR2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeiyi Wang,Zekun Shen,Yanan Tang,Bingyi Chen,Jinxing Chen,Jiaxuan Hou,Jiayan Li,Mengzhao Zhang,Shuang Liu,Yifan Mei,Liwei Zhang,Shaoying LuPubMed 35026516
- Biochemical pharmacology 195:1148642021Dictamnine, a novel c-Met inhibitor, suppresses the proliferation of lung cancer cells by downregulating the PI3K/AKT/mTOR and MAPK signaling pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiaojiao Yu,Lijing Zhang,Jun Peng,Richard Ward,Peiqi Hao,Jiwei Wang,Na Zhang,Yang Yang,Xiaoxi Guo,Cheng Xiang,Su An,Tian-Rui XuPubMed 34861243
- Journal of clinical laboratory analysis 35:e240442021miR-423-3p activates FAK signaling pathway to drive EMT process and tumor growth in lung adenocarcinoma through targeting CYBRD1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Ma,Wuhao Huang,Chaonan Zhu,Xiaoyan Sun,Qiang Zhang,Lianmin Zhang,Qi Qi,Xiaoming Bai,Yun Feng,Changli WangPubMed 34714955
- Molecular therapy. Nucleic acids 25:567-5772021miR-497 defect contributes to gastric cancer tumorigenesis and progression via regulating CDC42/ITGB1/FAK/PXN/AKT signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLihui Zhang,Liwen Yao,Wei Zhou,Jinping Tian,Banlai Ruan,Zihua Lu,Yunchao Deng,Qing Li,Zhi Zeng,Dongmei Yang,Renduo Shang,Ming Xu,Mengjiao Zhang,Du Cheng,Yanning Yang,Qianshan Ding,Honggang YuPubMed 34589278
- Nature communications 9:51052018Cytoplasmic LIF reprograms invasive mode to enhance NPC dissemination through modulating YAP1-FAK/PXN signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShu-Chen Liu et. al.PubMed 30504771
- Oncotarget 7:72113-721302016Myocardin-related transcription factor A (MRTF-A) activity-dependent cell adhesion is correlated to focal adhesion kinase (FAK) activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakayuki Kishi,Taira Mayanagi,Sadahiro Iwabuchi,Toshihide Akasaka,Kenji SobuePubMed 27708220
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | Dot | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Expected | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Predicted | Predicted | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Predicted | Predicted | Not recommended | Not recommended |
| Synthetic peptide | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
Target data
Function
Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Recruits other proteins such as TRIM15 to focal adhesion.
Alternative names
Paxillin, PXN
Recommended products
Rabbit Recombinant Monoclonal Paxillin phospho Y118 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples. Cited in 7 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR1903
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Paxillin also known as PXN is a cytoskeletal protein involved in the assembly of focal adhesions. It has a molecular weight of approximately 68 kDa. You will find Paxillin expressed widely in various tissues including the liver colon and lung. Paxillin plays a part in cell motility by interacting with other focal adhesion proteins. It plays a significant mechanical role by serving as a docking site for structural and signaling molecules.
- Biological function summary
Paxillin serves key functions in cellular processes such as migration proliferation and survival. It interacts with different proteins including vinculin and talin forming part of a complex at the focal adhesion sites. The phosphorylation state of Paxillin can modulate its interactions and in this way influence the assembly of signaling complexes that control dynamic cellular processes.
- Pathways
Paxillin participates in the integrin signaling and MAPK pathways. It operates by linking integrin receptors to the actin cytoskeleton facilitating signal transduction. Paxillin phosphorylation is an important regulatory mechanism within these pathways. In particular its interaction with focal adhesion kinase (FAK) and Src family kinases signifies its role in transmitting signals from the extracellular matrix to the cellular interior which impacts cell behavior and response.
- Associated diseases and disorders
Paxillin has links with osteosarcoma and lung cancer. Deregulation of Paxillin expression or its phosphorylation state contributes to oncogenic transformation and tumor progression. In cancer Paxillin associates with altered activity of proteins such as Src and FAK exacerbating disease states. This relationship highlights Paxillin's potential as a biomarker and therapeutic target in treating related malignancies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547), expandable thumbnail](https://content.abcam.com/products/images/paxillin-phospho-y118-antibody-epr1903-ab109547--western-blot-img20182.jpg "Western blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547)")
Western blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547)
Paxillin (phospho Y118) Western blot staining using rabbit Anti-Paxillin (phospho Y118) antibody
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547) at 1/1000 dilution
Lane 1: Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 10 µg
Lane 2: Jurkat treated with 50mM pervanadate for 5 min whole cell lysate at 10 µg
Lane 3: Jurkat treated with 50mM pervanadate for 5 min whole cell lysate, then the membrane was incubated with alkaline phosphatase at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDa
![Dot Blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547), expandable thumbnail](https://content.abcam.com/products/images/paxillin-phospho-y118-antibody-epr1903-ab109547--dot-blot-img18149.jpg "Dot Blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547)")
Dot Blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547)
Dot blot analysis of Paxillin (phospho Y118) phospho peptide (Lane 1) and Paxillin phospho peptide (Lane 2) labelling Paxillin (phospho Y118) with ab109547 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as the secondary antibody at a dilution of 1/20,000. Blocking buffer: 5% NFDM/BST. Dilution buffer: 5% NFDM /TBST.
![Western blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547), expandable thumbnail](https://content.abcam.com/products/images/paxillin-phospho-y118-antibody-epr1903-ab109547--western-blot-img69574.jpg "Western blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547)")
Western blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547)
All lanes: Western blot - Anti-Paxillin (phospho Y118) antibody [EPR1903] (ab109547) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: HeLa cell lysate treated with pervanadate at 10 µg
Predicted band size: 46 kDa, 65 kDa
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