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AB4832

Anti-Paxillin (phospho Y31) antibody

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(9 Publications)

Rabbit Polyclonal Paxillin phospho Y31 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human Paxillin phospho Y31.

View Alternative Names

Paxillin, PXN

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Paxillin (phospho Y31) antibody (AB4832)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Paxillin (phospho Y31) antibody (AB4832)

Colocalization of phospho Paxillin and actin in TGF-beta treated NMμMG (mouse mammary) cells. Green = actin stress fibers, Orange = Paxillin [pY31], Yellow = colocalization.

Western blot - Anti-Paxillin (phospho Y31) antibody (AB4832)
  • WB

Supplier Data

Western blot - Anti-Paxillin (phospho Y31) antibody (AB4832)

ab4832 was diluted between 1/500 and 1/2000.

All lanes:

Western blot - Anti-Paxillin (phospho Y31) antibody (ab4832)

Lane 1:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 20 µL

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell lysate treated for 1 Hr with 100 μM of Pervanadate at 20 µL

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µL

Lane 4:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate treated for 20 minutes with 50 ng/mL of TNF alpha at 20 µL

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 20 µL

Lane 6:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate treated for 10 minutes with 50 ng/mL of PDGF at 20 µL

Lane 7:

A549 (human lung carcinoma cell line) whole cell lysate at 20 µL

Lane 8:

A549 (human lung carcinoma cell line) whole cell lysate treated for 20 minutes with 200 nM of PMA at 20 µL

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) HRP conjugate at 1/2500 dilution

Predicted band size: 65 kDa

Observed band size: 60 kDa

true

Immunocytochemistry/ Immunofluorescence - Anti-Paxillin (phospho Y31) antibody (AB4832)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Paxillin (phospho Y31) antibody (AB4832)

Immunocytochemistry/ Immunofluorescence of NIH/3T3 (mouse embryo fibroblast cell line) treated with 50 ng of PDGF for 10 minutes with ab4832 (1/250). A Goat anti-Rabbit IgG (H+L) Alexa Fluor® 488 conjugate (1/2000) was used as a secondary (Green). DAPI (Blue) was used as a counter stain. F-actin was stained with Alexa Fluor® 555 Rhodamine Phalloidin (1/300). Panel e is untreaded cells and panel f had no primary antibody.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human Paxillin phospho Y31. The exact immunogen used to generate this antibody is proprietary information.

P49023

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated paxillin, and ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using a paxillin-derived peptide that is phosphorylated at tyrosine 31.
Storage buffer
pH: 7.3 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Paxillin also known as PXN is a cytoskeletal protein involved in the assembly of focal adhesions. It has a molecular weight of approximately 68 kDa. You will find Paxillin expressed widely in various tissues including the liver colon and lung. Paxillin plays a part in cell motility by interacting with other focal adhesion proteins. It plays a significant mechanical role by serving as a docking site for structural and signaling molecules.
Biological function summary

Paxillin serves key functions in cellular processes such as migration proliferation and survival. It interacts with different proteins including vinculin and talin forming part of a complex at the focal adhesion sites. The phosphorylation state of Paxillin can modulate its interactions and in this way influence the assembly of signaling complexes that control dynamic cellular processes.

Pathways

Paxillin participates in the integrin signaling and MAPK pathways. It operates by linking integrin receptors to the actin cytoskeleton facilitating signal transduction. Paxillin phosphorylation is an important regulatory mechanism within these pathways. In particular its interaction with focal adhesion kinase (FAK) and Src family kinases signifies its role in transmitting signals from the extracellular matrix to the cellular interior which impacts cell behavior and response.

Paxillin has links with osteosarcoma and lung cancer. Deregulation of Paxillin expression or its phosphorylation state contributes to oncogenic transformation and tumor progression. In cancer Paxillin associates with altered activity of proteins such as Src and FAK exacerbating disease states. This relationship highlights Paxillin's potential as a biomarker and therapeutic target in treating related malignancies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Recruits other proteins such as TRIM15 to focal adhesion.
See full target information Paxillin phospho Y31
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 49: PubMed35266010

2022

Potential mechanisms of osteoprotegerin-induced damage to osteoclast adhesion structures via P2X7R-mediated MAPK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yonggang Ma,Xueni Shi,Hongyan Zhao,Ruilong Song,Hui Zou,Jiaqiao Zhu,Zongping Liu

Frontiers in cardiovascular medicine 8:686200 PubMed34746246

2021

Galectin-3 Mediates Thrombin-Induced Vascular Smooth Muscle Cell Migration.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Tian,Chun-Kai Huang,Fenghua Ding,Ruiyan Zhang

American journal of physiology. Cell physiology 320:C1013-C1030 PubMed33788628

2021

TRPV4-Rho signaling drives cytoskeletal and focal adhesion remodeling in trabecular meshwork cells.

Applications

Unspecified application

Species

Unspecified reactive species

Monika Lakk,David Križaj

Neuroscience 448:160-171 PubMed33002558

2020

Fmn2 Regulates Growth Cone Motility by Mediating a Molecular Clutch to Generate Traction Forces.

Applications

Unspecified application

Species

Unspecified reactive species

Ketakee Ghate,Sampada P Mutalik,Lakshmi Kavitha Sthanam,Shamik Sen,Aurnab Ghose

ACS applied materials & interfaces 11:28377-28386 PubMed31251577

2019

Controlling the Multiscale Network Structure of Fibers To Stimulate Wound Matrix Rebuilding by Fibroblast Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Li,Zecong Xiao,Yajiao Zhou,Sen Zheng,Ying An,Wen Huang,Huacheng He,Yao Yang,Shengyu Li,Yanxin Chen,Jian Xiao,Jiang Wu

The EMBO journal 34:448-65 PubMed25471072

2014

KSHV-TK is a tyrosine kinase that disrupts focal adhesions and induces Rho-mediated cell contraction.

Applications

Unspecified application

Species

Unspecified reactive species

Michael B Gill,Rachel Turner,Philip G Stevenson,Michael Way

Cancer biology & therapy 14:679-91 PubMed23792636

2013

Paxillin mutations affect focal adhesions and lead to altered mitochondrial dynamics: relevance to lung cancer.

Applications

IHC

Species

Unspecified reactive species

Ichiro Kawada,Rifat Hasina,Frances E Lennon,Vytautas P Bindokas,Peter Usatyuk,Yi-Hung C Tan,Soundararajan Krishnaswamy,Qudsia Arif,George Carey,Robyn D Hseu,Matthew Robinson,Maria Tretiakova,Toni M Brand,Mari Iida,Mark K Ferguson,Deric L Wheeler,Aliya N Husain,Viswanathan Natarajan,Everett E Vokes,Patrick A Singleton,Ravi Salgia

Molecular cancer therapeutics 5:2388-97 PubMed16985073

2006

Inhibition of c-Met and prevention of spontaneous metastatic spreading by the 2-indolinone RPI-1.

Applications

WB

Species

Human

Giuliana Cassinelli,Cinzia Lanzi,Giovanna Petrangolini,Monica Tortoreto,Graziella Pratesi,Giuditta Cuccuru,Diletta Laccabue,Rosanna Supino,Sara Belluco,Enrica Favini,Anna Poletti,Franco Zunino

Oncogene 24:1084-90 PubMed15592501

2004

Met proto-oncogene juxtamembrane rare variations in mouse and humans: differential effects of Arg and Cys alleles on mouse lung tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Daniela Zaffaroni,Monica Spinola,Antonella Galvan,F Stefania Falvella,Simonetta Pazzaglia,Anna Saran,Maria Teresa Mancuso,Federica Galbiati,Carmen Pignatiello,Wafa Cabrera,Olga Ibanez,Giacomo Manenti,Tommaso A Dragani
View all publications
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