JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB226923

Anti-PBK/SPK antibody

Be the first to review this product! Submit a review

|

(3 Publications)

Rabbit Polyclonal PBK/SPK antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PBK.

View Alternative Names

TOPK, PBK, Lymphokine-activated killer T-cell-originated protein kinase, Cancer/testis antigen 84, MAPKK-like protein kinase, Nori-3, PDZ-binding kinase, Spermatogenesis-related protein kinase, T-LAK cell-originated protein kinase, CT84, SPK

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-PBK/SPK antibody (AB226923)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PBK/SPK antibody (AB226923)

4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling PBK/SPK (green) using ab226923 at 1/500 dilution in ICC/IF. Nuclear counterstained with Hoechst 33342 (blue).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IP, WB

applications

Immunogen

Recombinant Fragment Protein within Human PBK. The exact immunogen used to generate this antibody is proprietary information.

Q96KB5

style
left-column

Alternative Products

Primary Antibodies

AB184953

Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (AB184953)

  • 0
  • 0
Primary Antibodies

AB75987

Anti-PBK/SPK antibody [EP2520Y]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK antibody [EP2520Y] (AB75987)

  • 0
  • 0
Primary Antibodies

AB236872

Anti-PBK/SPK antibody [EPR21983]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK antibody [EPR21983] (AB236872)

  • 0
  • 0
Primary Antibodies

AB239760

Anti-PBK/SPK antibody [EPR21983] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK antibody [EPR21983] - BSA and Azide free (AB239760)

  • 0
  • 0
Primary Antibodies

AB236871

Anti-PBK/SPK antibody [EPR21982]

RabMAb|Recombinant|KO Validated|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK antibody [EPR21982] (AB236871)

  • 0
  • 0
Primary Antibodies

AB239756

Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (AB239756)

  • 0
  • 0

Complementary Products

Assay Kits

AB184866

Human PBK ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human PBK ELISA Kit (AB184866)

  • 0
  • 0
Primary Antibodies

AB184953

Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (AB184953)

  • 0
  • 0
Primary Antibodies

AB26483

Anti-Cdc20 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Cdc20 antibody (AB26483)

  • 5
  • 1
Primary Antibodies

AB133327

Anti-CDK1 antibody [EPR165]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CDK1 antibody [EPR165] (AB133327)

  • 5
  • 4
Proteins & Peptides

AB70619

Recombinant human PBK/SPK protein

SDS-PAGE - Recombinant human PBK/SPK protein (AB70619)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/1000", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PBK/SPK also known as PDZ-binding kinase or Cdc2-related kinase is a serine/threonine protein kinase with an approximate molecular weight of 47 kDa. This protein is involved in cell division playing an important role in mitotic processes. PBK/SPK is expressed in various tissues including significant levels in testis and placenta often in proliferating cell types. It also exhibits higher expression in cancerous tissues suggesting a role in tumorigenesis.
Biological function summary

PBK/SPK facilitates cell cycle progression and cellular proliferation. This protein is not typically part of larger complexes but it partners with other molecules during cell division to ensure proper mitotic spindle formation and chromosome segregation. Its expression levels increase during the G2/M phase of the cell cycle indicating its involvement in cell cycle regulation. In cancer it often shows overexpression contributing to unregulated proliferation of cancer cells.

Pathways

PBK/SPK is associated with critical cell division pathways such as the MAPK signaling pathway. This involvement impacts cellular responses to growth signals and stress. PBK can phosphorylate and interact with proteins like p38 MAPK which further modulates signal transduction related to cellular growth and survival. Through these pathways PBK/SPK helps manage the balance between cell division and apoptosis particularly under stress conditions.

PBK/SPK has strong correlations with cancer especially glioblastoma and cervical cancer. Overexpression of PBK often links to poor prognosis in these conditions. In cancerous settings PBK interacts with proteins such as p53 and c-Myc influencing tumor progression and resistance to therapy. PBK can modulate oncogenic pathways providing a potential target for therapeutic intervention in tumor proliferation and survival.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
See full target information PBK
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

ACS applied bio materials 6:1787-1797 PubMed37126648

2023

Collagen Gels Crosslinked by Photoactivation of Riboflavin for the Repair and Regeneration of Corneal Defects.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriella Maria Fernandes-Cunha,Lucia G Brunel,Alejandro Arboleda,Alyssa Manche,Youngyoon Amy Seo,Caitlin Logan,Fang Chen,Sarah C Heilshorn,David Myung

Cancer research communications 2:1617-1625 PubMed36970725

2022

Novel Paired Normal Prostate and Prostate Cancer Model Cell Systems Derived from African American Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Mira Jung,Keith Kowalczyk,Ryan Hankins,Gaurav Bandi,Bhaskar Kallakury,Michael A Carrasquilla,Partha P Banerjee,Scott Grindrod,Anatoly Dritschilo

Translational oncology 13:287-294 PubMed31874375

2019

PDZ-Binding Kinase-Dependent Transcriptional Regulation of CCNB2 Promotes Tumorigenesis and Radio-Resistance in Glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Mao,Gang Bao,Yi-Chang Wang,Chang-Wang Du,Xiao Yu,Xiao-Ye Guo,Rui-Chun Li,Mao-De Wang
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com