Rabbit Recombinant Monoclonal PBK/SPK antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Rat samples.
Images
![Western blot - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (AB239756), expandable thumbnail](https://content.abcam.com/products/images/pbk-spk-antibody-epr21982-bsa-and-azide-free-ab239756--western-blot-img142868.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (AB239756)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (AB239756), expandable thumbnail](https://content.abcam.com/products/images/pbk-spk-antibody-epr21982-bsa-and-azide-free-ab239756--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130031.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (AB239756)")
![Immunoprecipitation - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (AB239756), expandable thumbnail](https://content.abcam.com/products/images/pbk-spk-antibody-epr21982-bsa-and-azide-free-ab239756--immunoprecipitation-img3987.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (AB239756)")
![Western blot - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (AB239756), expandable thumbnail](https://content.abcam.com/products/images/pbk-spk-antibody-epr21982-bsa-and-azide-free-ab239756--western-blot-img53570.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (AB239756)")
![Western blot - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (AB239756), expandable thumbnail](https://content.abcam.com/products/images/pbk-spk-antibody-epr21982-bsa-and-azide-free-ab239756--western-blot-img125094.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (AB239756)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Rat | Expected | Tested | Expected | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes IHC is recommended for human only. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
Alternative names
TOPK, PBK, Lymphokine-activated killer T-cell-originated protein kinase, Cancer/testis antigen 84, MAPKK-like protein kinase, Nori-3, PDZ-binding kinase, Spermatogenesis-related protein kinase, T-LAK cell-originated protein kinase, CT84, SPK
Recommended products
Rabbit Recombinant Monoclonal PBK/SPK antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR21982
- Purification technique
- Affinity purification Protein A
- Specificity
IHC is recommended for human only.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab239756 is the carrier-free version of Anti-PBK/SPK antibody [EPR21982] ab236871.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PBK/SPK also known as PDZ-binding kinase or Cdc2-related kinase is a serine/threonine protein kinase with an approximate molecular weight of 47 kDa. This protein is involved in cell division playing an important role in mitotic processes. PBK/SPK is expressed in various tissues including significant levels in testis and placenta often in proliferating cell types. It also exhibits higher expression in cancerous tissues suggesting a role in tumorigenesis.
- Biological function summary
PBK/SPK facilitates cell cycle progression and cellular proliferation. This protein is not typically part of larger complexes but it partners with other molecules during cell division to ensure proper mitotic spindle formation and chromosome segregation. Its expression levels increase during the G2/M phase of the cell cycle indicating its involvement in cell cycle regulation. In cancer it often shows overexpression contributing to unregulated proliferation of cancer cells.
- Pathways
PBK/SPK is associated with critical cell division pathways such as the MAPK signaling pathway. This involvement impacts cellular responses to growth signals and stress. PBK can phosphorylate and interact with proteins like p38 MAPK which further modulates signal transduction related to cellular growth and survival. Through these pathways PBK/SPK helps manage the balance between cell division and apoptosis particularly under stress conditions.
- Associated diseases and disorders
PBK/SPK has strong correlations with cancer especially glioblastoma and cervical cancer. Overexpression of PBK often links to poor prognosis in these conditions. In cancerous settings PBK interacts with proteins such as p53 and c-Myc influencing tumor progression and resistance to therapy. PBK can modulate oncogenic pathways providing a potential target for therapeutic intervention in tumor proliferation and survival.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
![Western blot - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756), expandable thumbnail](https://content.abcam.com/products/images/pbk-spk-antibody-epr21982-bsa-and-azide-free-ab239756--western-blot-img142868.jpg "Western blot - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756)")
Western blot - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PBK/SPK antibody [EPR21982] ab236871).
Lanes 1- 2: Merged signal (red and green). Green - Anti-PBK/SPK antibody [EPR21982] ab236871 observed at 40 kDa. Red - Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) observed at 50 kDa.
Anti-PBK/SPK antibody [EPR21982] ab236871 was shown to react with PBK/SPK in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line Human PBK (SPK) knockout HEK-293T cell line ab266827 (knockout cell lysate Human PBK (SPK) knockout HEK-293T cell lysate ab257575) was used. Wild-type HEK-293T and PBK knockout HEK-293T cell lysates were subjected to SDS-PAGE. Anti-PBK/SPK antibody [EPR21982] ab236871 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-PBK/SPK antibody [EPR21982] (Anti-PBK/SPK antibody [EPR21982] ab236871) at 1/1000 dilution
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: PBK knockout HEK-293T cell lysate at 20 µg
Lane 2: Western blot - Human PBK (SPK) knockout HEK-293T cell line (Human PBK (SPK) knockout HEK-293T cell line ab266827)
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 40 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756), expandable thumbnail](https://content.abcam.com/products/images/pbk-spk-antibody-epr21982-bsa-and-azide-free-ab239756--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130031.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756)
Immunohistochemical analysis of paraffin-embedded human testis tissue labeling PBK with Anti-PBK/SPK antibody [EPR21982] ab236871 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in human esophagus cancer (PMID: 27919968). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PBK/SPK antibody [EPR21982] ab236871).
![Immunoprecipitation - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756), expandable thumbnail](https://content.abcam.com/products/images/pbk-spk-antibody-epr21982-bsa-and-azide-free-ab239756--immunoprecipitation-img3987.jpg "Immunoprecipitation - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756)")
Immunoprecipitation - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756)
PBK/SPK was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with Anti-PBK/SPK antibody [EPR21982] ab236871 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-Fyn antibody [EPR19636] ab184276 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.
Lane 1: HeLa whole cell lysate 10 μg (Input).
Lane 2: ab231871 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab231871 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PBK/SPK antibody [EPR21982] ab236871).
All lanes: Immunoprecipitation - Anti-PBK/SPK antibody [EPR21982] (Anti-PBK/SPK antibody [EPR21982] ab236871)
Predicted band size: 36 kDa, 50 kDa
Observed band size: 36 kDa, 46 kDa, 50 kDa
![Western blot - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756), expandable thumbnail](https://content.abcam.com/products/images/pbk-spk-antibody-epr21982-bsa-and-azide-free-ab239756--western-blot-img53570.jpg "Western blot - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756)")
Western blot - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756)
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID:23547718; PMID:25909225).
Anti-PBK/SPK antibody [EPR21982] ab236871 was shown to specifically react with PBK/SPK in wild-type HAP1 cells as signal was lost in PBK/SPK knockout cells. Wild-type and PBK/SPK knockout samples were subjected to SDS-PAGE. Anti-PBK/SPK antibody [EPR21982] ab236871 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (Anti-PBK/SPK antibody [EPR21982] ab236871).
All lanes: Western blot - Anti-PBK/SPK antibody [EPR21982] (Anti-PBK/SPK antibody [EPR21982] ab236871) at 1/1000 dilution
Lane 1: Wild-type HAP1 cell lysate at 20 µg
Lane 2: PBK/SPK knockout HAP1 cell lysate at 20 µg
Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 4: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 36 kDa
Observed band size: 38 kDa
Exposure time: 15s
![Western blot - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756), expandable thumbnail](https://content.abcam.com/products/images/pbk-spk-antibody-epr21982-bsa-and-azide-free-ab239756--western-blot-img125094.jpg "Western blot - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756)")
Western blot - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756)
Exposure time: Lanes 1-3: 26 seconds; Lane 4: 8 seconds; Lanes 5-6: 70 seconds.Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID:23547718; PMID:25909225).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PBK/SPK antibody [EPR21982] ab236871).
All lanes: Western blot - Anti-PBK/SPK antibody [EPR21982] (Anti-PBK/SPK antibody [EPR21982] ab236871) at 1/1000 dilution
Lane 1: SW480 (human colorectal adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 2: A431 (human epidermoid carcinoma cell line) whole cell lysate at 20 µg
Lane 3: U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 20 µg
Lane 4: Rat testis lysate at 20 µg
Lane 5: C6 (rat glial tumor cell line) whole cell lysate at 10 µg
Lane 6: PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 36 kDa
Observed band size: 38 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756), expandable thumbnail](https://content.abcam.com/products/images/pbk-spk-antibody-epr21982-bsa-and-azide-free-ab239756--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124214.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756)
Immunohistochemical analysis of paraffin-embedded human esophagus cancer tissue labeling PBK/SPK with Anti-PBK/SPK antibody [EPR21982] ab236871 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in human esophagus cancer (PMID: 27919968) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PBK/SPK antibody [EPR21982] ab236871).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756), expandable thumbnail](https://content.abcam.com/products/images/pbk-spk-antibody-epr21982-bsa-and-azide-free-ab239756--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img59241.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756)
Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling PBK/SPK with Anti-PBK/SPK antibody [EPR21982] ab236871 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in human gastric cancer (PMID:26894977; PMID:27898655) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PBK/SPK antibody [EPR21982] ab236871).
![Flow Cytometry (Intracellular) - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756), expandable thumbnail](https://content.abcam.com/products/images/pbk-spk-antibody-epr21982-bsa-and-azide-free-ab239756--flow-cytometry-intracellular-img414203.jpg "Flow Cytometry (Intracellular) - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756)")
Flow Cytometry (Intracellular) - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling PBK/SPK with Anti-PBK/SPK antibody [EPR21982] ab236871 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PBK/SPK antibody [EPR21982] ab236871).
![Flow Cytometry (Intracellular) - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756), expandable thumbnail](https://content.abcam.com/products/images/pbk-spk-antibody-epr21982-bsa-and-azide-free-ab239756--flow-cytometry-intracellular-img413738.jpg "Flow Cytometry (Intracellular) - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756)")
Flow Cytometry (Intracellular) - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cell line labeling PBK/SPK with Anti-PBK/SPK antibody [EPR21982] ab236871 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PBK/SPK antibody [EPR21982] ab236871).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756), expandable thumbnail](https://content.abcam.com/products/images/pbk-spk-antibody-epr21982-bsa-and-azide-free-ab239756--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124214.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756), expandable thumbnail](https://content.abcam.com/products/images/pbk-spk-antibody-epr21982-bsa-and-azide-free-ab239756--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img59241.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756)")
![Flow Cytometry (Intracellular) - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756), expandable thumbnail](https://content.abcam.com/products/images/pbk-spk-antibody-epr21982-bsa-and-azide-free-ab239756--flow-cytometry-intracellular-img414203.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756)")
![Flow Cytometry (Intracellular) - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756), expandable thumbnail](https://content.abcam.com/products/images/pbk-spk-antibody-epr21982-bsa-and-azide-free-ab239756--flow-cytometry-intracellular-img413738.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-PBK/SPK antibody [EPR21982] - BSA and Azide free (ab239756)")
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