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Rabbit Recombinant Monoclonal PBK/SPK phospho T9 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human, Rat samples. Cited in 1 publication.

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Images

Western blot - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] - BSA and Azide free (AB250790), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] - BSA and Azide free (AB250790), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] - BSA and Azide free (AB250790), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] - BSA and Azide free (AB250790), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] - BSA and Azide free (AB250790), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Expected
Mouse
Predicted
Predicted
Predicted
Rat
Expected
Expected
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Target data

Function

Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.

Alternative names

TOPK, PBK, Lymphokine-activated killer T-cell-originated protein kinase, Cancer/testis antigen 84, MAPKK-like protein kinase, Nori-3, PDZ-binding kinase, Spermatogenesis-related protein kinase, T-LAK cell-originated protein kinase, CT84, SPK

Recommended products

Rabbit Recombinant Monoclonal PBK/SPK phospho T9 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human, Rat samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR2475(N)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab250790 is the carrier-free version of Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] ab184953.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PBK/SPK also known as PDZ-binding kinase or Cdc2-related kinase is a serine/threonine protein kinase with an approximate molecular weight of 47 kDa. This protein is involved in cell division playing an important role in mitotic processes. PBK/SPK is expressed in various tissues including significant levels in testis and placenta often in proliferating cell types. It also exhibits higher expression in cancerous tissues suggesting a role in tumorigenesis.

Biological function summary

PBK/SPK facilitates cell cycle progression and cellular proliferation. This protein is not typically part of larger complexes but it partners with other molecules during cell division to ensure proper mitotic spindle formation and chromosome segregation. Its expression levels increase during the G2/M phase of the cell cycle indicating its involvement in cell cycle regulation. In cancer it often shows overexpression contributing to unregulated proliferation of cancer cells.

Pathways

PBK/SPK is associated with critical cell division pathways such as the MAPK signaling pathway. This involvement impacts cellular responses to growth signals and stress. PBK can phosphorylate and interact with proteins like p38 MAPK which further modulates signal transduction related to cellular growth and survival. Through these pathways PBK/SPK helps manage the balance between cell division and apoptosis particularly under stress conditions.

Associated diseases and disorders

PBK/SPK has strong correlations with cancer especially glioblastoma and cervical cancer. Overexpression of PBK often links to poor prognosis in these conditions. In cancerous settings PBK interacts with proteins such as p53 and c-Myc influencing tumor progression and resistance to therapy. PBK can modulate oncogenic pathways providing a potential target for therapeutic intervention in tumor proliferation and survival.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] - BSA and Azide free (ab250790), expandable thumbnail

    Western blot - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] - BSA and Azide free (ab250790)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] ab184953).

    All lanes: Western blot - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] (Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] ab184953) at 1/5000 dilution

    Lane 1: HeLa cell lysate treated with Nocodazole at 10 µg

    Lane 2: HeLa cell lysate untreated at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 36 kDa

    Observed band size: 40 kDa

  • Flow Cytometry (Intracellular) - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] - BSA and Azide free (ab250790), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] - BSA and Azide free (ab250790)

    Intracellular flow cytometric analysis of4% paraformaldehyde-fixed, 90% methanol-permeabilised HeLa (Human cervix adenocarcinoma epithelial cell) treated with 0.1μg/mL Nocodazole for 24h (Red) / Untreated control (Green) cells labeling PBK/SPK (phospho T9) with Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] ab184953 at 1/800 dilution compared with a Isotype control Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody. Higher expression in part of Nocodazole treated samples (mitotic cell induction) which is consistent with cell cycle Flow Cytometry data.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] ab184953).

  • Flow Cytometry (Intracellular) - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] - BSA and Azide free (ab250790), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] - BSA and Azide free (ab250790)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PBK/SPK (phospho T9) with Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] ab184953 at 1/800 dilution (Right) compared with a Rabbit monoclonal IgG Isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Left). Cells permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody. Highest expression at M phase.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] ab184953).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] - BSA and Azide free (ab250790), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] - BSA and Azide free (ab250790)

    Immunohistochemical analysis of paraffin-embedded human testis tissue labeling PBK/SPK (phospho T9) with Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] ab184953 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] ab184953).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] - BSA and Azide free (ab250790), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] - BSA and Azide free (ab250790)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling PBK/SPK (phospho T9) with Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] ab184953 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PBK/SPK (phospho T9) antibody [EPR2475(N)] ab184953).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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