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AB154878

Anti-PBR antibody

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(7 Publications)

Rabbit Polyclonal PBR antibody. Suitable for IHC-P and reacts with Rat samples. Cited in 7 publications.

View Alternative Names

Bzrp, Mbr, Tspo, Translocator protein, Mitochondrial benzodiazepine receptor, PKBS, Peripheral-type benzodiazepine receptor, PBR

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBR antibody (AB154878)
  • IHC-P

Ap

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBR antibody (AB154878)

IHC image of TPBR staining in normal Rat testes formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab154878, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chinese hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Macaque monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PBR also known as the Peripheral Benzodiazepine Receptor is a protein predominantly found in the outer mitochondrial membrane. It has alternate names like Translocator Protein (TSPO) and the mass of this protein is approximately 18 kDa. PBR is expressed in various tissues but shows high levels in steroidogenic tissues like adrenal glands as well as in the brain heart liver and kidneys. The abundant presence in these tissues highlights its importance in a variety of physiological functions.
Biological function summary

PBR interacts with cholesterol for the synthesis of steroid hormones making it important for steroidogenesis. PBR is a part of the Mitochondrial Permeability Transition Pore complex (MPTP) involved in regulating the transport of molecules across the mitochondrial membrane. Through its association with the MPTP PBR plays a significant role in mitochondrial functions such as apoptosis and energy metabolism. The interaction with other molecules also includes the binding with benzodiazepines impacting processes like immune response and cell proliferation.

Pathways

PBR plays a role in the steroid biosynthesis and apoptosis pathways. It interfaces with the StAR (Steroidogenic Acute Regulatory) protein to facilitate cholesterol transport into mitochondria the initial step in steroid hormone production. PBR is also involved in pathways that regulate apoptosis and mitochondrial function linking it to different cellular processes through interactions with proteins like VDAC (Voltage-Dependent Anion Channel).

PBR has relevance to conditions such as neurodegenerative diseases and cancer. PBR expression changes in disorders like Alzheimer's disease where it might reflect mitochondrial dysfunctions. It has also been associated with certain cancers where aberrant PBR activity might contribute to altered cell proliferation and apoptosis. Proteins like caspases are involved in the apoptotic pathways connected with PBR highlighting its involvement in disease processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Promotes the transport of cholesterol across mitochondrial membranes and may play a role in lipid metabolism, but its precise physiological role is controversial. It is apparently not required for steroid hormone biosynthesis (By similarity). Can bind protoporphyrin IX and may play a role in the transport of porphyrins and heme. Was initially identified as peripheral-type benzodiazepine receptor; can also bind isoquinoline carboxamides (PubMed : 2555358).
See full target information Tspo
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Basic research in cardiology 119:481-503 PubMed38517482

2024

Identification of a mechanism promoting mitochondrial sterol accumulation during myocardial ischemia-reperfusion: role of TSPO and STAR.

Applications

Unspecified application

Species

Unspecified reactive species

Juliette Bréhat,Shirin Leick,Julien Musman,Jin Bo Su,Nicolas Eychenne,Frank Giton,Michael Rivard,Louis-Antoine Barel,Chiara Tropeano,Frederica Vitarelli,Claudio Caccia,Valerio Leoni,Bijan Ghaleh,Sandrine Pons,Didier Morin

Journal of clinical medicine 12: PubMed36614916

2022

Evaluation of -[C]PK11195 PET/MRI for Spinal Cord-Related Neuropathic Pain in Patients with Cervical Spinal Disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Makoto Kitade,Hideaki Nakajima,Tetsuya Tsujikawa,Sakon Noriki,Tetsuya Mori,Yasushi Kiyono,Hidehiko Okazawa,Akihiko Matsumine

Parkinson's disease 2022:3506213 PubMed36105302

2022

The Anti-Inflammatory Effect of Preventive Intervention with Ketogenic Diet Mediated by the Histone Acetylation of Promotor Region in Rat Parkinson's Disease Model: A Dual-Tracer PET Study.

Applications

Unspecified application

Species

Unspecified reactive species

Yuankai Zhu,Xiangyu Tang,Zhaoting Cheng,Qingjian Dong,Ge Ruan

EJNMMI research 10:113 PubMed32990808

2020

TSPO PET detects acute neuroinflammation but not diffuse chronically activated MHCII microglia in the rat.

Applications

Unspecified application

Species

Unspecified reactive species

Nassir U Al-Khishman,Qi Qi,Austyn D Roseborough,Alexander Levit,Brian L Allman,Udunna C Anazodo,Matthew S Fox,Shawn N Whitehead,Jonathan D Thiessen

The Journal of endocrinology 247:1-10 PubMed32698131

2020

Lack of adrenal TSPO/PBR expression in hamsters reinforces correlation to triglyceride metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Prasanthi P Koganti,Vimal Selvaraj

Molecular imaging and biology 21:669-675 PubMed30280292

2018

Monitoring the Progression of Chronic Liver Damage in Rats Using [F]PBR06.

Applications

Unspecified application

Species

Unspecified reactive species

Shuo Huang,Chao Li,Jun Guo,Linlin Zhang,Shuqi Wu,Hui Wang,Sheng Liang

The Biochemical journal 474:3985-3999 PubMed29074640

2017

mutations in rats and a human polymorphism impair the rate of steroid synthesis.

Applications

WB

Species

Rat

David R Owen,Jinjiang Fan,Enrico Campioli,Sathvika Venugopal,Andrew Midzak,Edward Daly,Aline Harlay,Leeyah Issop,Vincenzo Libri,Dimitra Kalogiannopoulou,Eduardo Oliver,Enrique Gallego-Colon,Alessandro Colasanti,Les Huson,Eugenii A Rabiner,Puvan Suppiah,Charles Essagian,Paul M Matthews,Vassilios Papadopoulos
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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