Anti-PBRM1/BAF180 antibody [BL-39-2C3] - BSA free

• Flow Cyt

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Flow Cytometry - Anti-PBRM1/BAF180 antibody [BL-39-2C3] - BSA free (AB272073)

Flow cytometry overlay histogram of 4% formaldehyde fixed Jurkat cells permeabilized with 90% methanol labeling human PBRM1 with ab243876 at 1 µl per 1 x 10^6 cells. (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883).

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243876).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBRM1/BAF180 antibody [BL-39-2C3] - BSA free (AB272073)

Formalin-fixed, paraffin-embedded human Breast tissue stained for PBRM1/BAF180 using ab243876 in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. Opal™ staining and DAPI counterstain.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243876).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBRM1/BAF180 antibody [BL-39-2C3] - BSA free (AB272073)

Immunohistochemical analysis of Formalin-Fixed Paraffin-Embedded human breast carcinoma labeling human PBRM1 with ab243876, followed by a HRP-conjugated goat anti-rabbit IgG as secondary. Substrate : DAB. Nuclear staining on breast carcinoma is observed. Counter stained with hematoxylin. Epitope retrieval with citrate buffer pH 6.0 for 20 minutes is recommended.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243876).

• ICC

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Immunocytochemistry - Anti-PBRM1/BAF180 antibody [BL-39-2C3] - BSA free (AB272073)

Immunocytochemical analysis of Formalin-Fixed Paraffin-Embedded MCF7 cells labeling human PBRM1 with ab243876 followed by a HRP-conjugated goat anti-rabbit IgG as secondary. Substrate : DAB. Nuclear staining on MCF7 cells is observed. Counter stained with hematoxylin. Epitope retrieval with citrate buffer pH 6.0 for 20 minutes is recommended.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243876).

• IP

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Immunoprecipitation - Anti-PBRM1/BAF180 antibody [BL-39-2C3] - BSA free (AB272073)

PBRM1/BAF180 was immunoprecipitated from 1 mg HEK-293T whole cell lysate with ab243876 at 20 μl per reaction Western blot was performed on the immunoprecipitate using ab243876 at 1/1000 dilution. Lysates prepared using NETN lysis buffer.

Detection : Chemiluminesence

Lane 1 : ab243876 IP in HEK-293T whole cell lysate.

Lane 2 : Contol IgG in HEK-293T whole cell lysate.

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243876).

All lanes:

Immunoprecipitation - Anti-PBRM1/BAF180 antibody [BL-39-2C3] (<a href='/en-us/products/primary-antibodies/pbrm1-baf180-antibody-bl-39-2c3-ab243876'>ab243876</a>)

Predicted band size: 193 kDa

false

Exposure time: 10s

• WB

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Western blot - Anti-PBRM1/BAF180 antibody [BL-39-2C3] - BSA free (AB272073)

Detection : Chemiluminescence

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243876).

All lanes:

Western blot - Anti-PBRM1/BAF180 antibody [BL-39-2C3] (<a href='/en-us/products/primary-antibodies/pbrm1-baf180-antibody-bl-39-2c3-ab243876'>ab243876</a>) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 50 µg

Lane 4:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 50 µg

Lane 5:

A549 (Human lung carcinoma cell line) whole cell lysate at 50 µg

Lane 6:

SW620 at 50 µg

Lane 7:

SK-MEL-28 at 50 µg

Lane 8:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 193 kDa

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-PBRM1/BAF180 antibody [BL-39-2C3] - BSA free (AB272073)

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243876).

All lanes:

Western blot - Anti-PBRM1/BAF180 antibody [BL-39-2C3] (<a href='/en-us/products/primary-antibodies/pbrm1-baf180-antibody-bl-39-2c3-ab243876'>ab243876</a>) at 1/1000 dilution

Lane 1:

BW5147.3 whole cell lysate at 50 µg

Lane 2:

EL4 whole cell lysate at 50 µg

Lane 3:

mIMCD-3 whole cell lysate at 50 µg

Lane 4:

RenCa whole cell lysate at 50 µg

Lane 5:

NIH 3T3 whole cell lysate at 50 µg

Lane 6:

TCMK-1 whole cell lysate at 50 µg

Lane 7:

C2C12 whole cell lysate at 50 µg

Lane 8:

A20 whole cell lysate at 50 µg

Lane 9:

Ba/F3 whole cell lysate at 50 µg

Lane 10:

CH27 whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Predicted band size: 187 kDa

Observed band size: 200 kDa

true

Exposure time: 30s

• WB

Supplier Data

Western blot - Anti-PBRM1/BAF180 antibody [BL-39-2C3] - BSA free (AB272073)

This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243876).

Lane 1:

Western blot - Anti-PBRM1/BAF180 antibody [BL-39-2C3] (<a href='/en-us/products/primary-antibodies/pbrm1-baf180-antibody-bl-39-2c3-ab243876'>ab243876</a>) at 1/10 dilution

Lane 2:

Western blot - Anti-PBRM1/BAF180 antibody [BL-39-2C3] (<a href='/en-us/products/primary-antibodies/pbrm1-baf180-antibody-bl-39-2c3-ab243876'>ab243876</a>) at 1/50 dilution

Lane 3:

Western blot - Anti-PBRM1/BAF180 antibody [BL-39-2C3] (<a href='/en-us/products/primary-antibodies/pbrm1-baf180-antibody-bl-39-2c3-ab243876'>ab243876</a>) at 1/250 dilution

All lanes:

NIH 3T3 whole cell lysate at 0.08 mg/mL

Predicted band size: 187 kDa

Observed band size: 200 kDa

true