AB240338

Anti-PBRM1/BAF180 antibody [EPR15860] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal PBRM1/BAF180 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human samples. Cited in 1 publication.

View Alternative Names

BAF180, PB1, PBRM1, Protein polybromo-1, hPB1, BRG1-associated factor 180, Polybromo-1D

3 Images

Flow Cytometry (Intracellular) - Anti-PBRM1/BAF180 antibody [EPR15860] - BSA and Azide free (AB240338)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-PBRM1/BAF180 antibody [EPR15860] - BSA and Azide free (AB240338)

ab196022 staining PBRM1/BAF180 in the human cell line HeLa (human cervix adenocarcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. The sample was incubated with the primary antibody at a dilution of 1/250. A goat anti rabbit IgG (Alexa Fluor^® 488) at a dilution of 1/2000 was used as the secondary antibody.

Isoytype control : Rabbit monoclonal IgG (Black)

Unlabelled control : Cell without incubation with primary antibody and secondary antibody (Blue)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab196022).

Immunocytochemistry/ Immunofluorescence - Anti-PBRM1/BAF180 antibody [EPR15860] - BSA and Azide free (AB240338)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PBRM1/BAF180 antibody [EPR15860] - BSA and Azide free (AB240338)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling PBRM1/BAF180 with ab196022 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). The nuclear counter stain is DAPI (blue).

Nuclear staining on HeLa cell line is observed.

-ve control - Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution. The nuclear counter stain is DAPI (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab196022).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBRM1/BAF180 antibody [EPR15860] - BSA and Azide free (AB240338)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBRM1/BAF180 antibody [EPR15860] - BSA and Azide free (AB240338)

This data was developed using ab196022, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PBRM1/BAF180 with ab196022 at 1/200 dilution.

Nuclear staining on human kidney

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Unconjugated

Anti-PBRM1/BAF180 antibody [EPR15860]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-PBRM1/BAF180 antibody [EPR15860]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15860

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

IHC-P, Flow Cyt (Intra), ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab240338 is the carrier-free version of ab196022.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PBRM1 also known as BAF180 is an important component of the SWI/SNF chromatin remodeling complex. It has a molecular weight of approximately 187 kDa. Within the cell PBRM1 is expressed in various tissues but shows higher expression in kidney and breast tissues. Its role involves binding to acetylated histones which influences chromatin structure and gene expression regulation.

Biological function summary

The PBRM1/BAF180 protein functions as part of the SWI/SNF complex which includes the catalytic subunit BRG1 or BRM. This complex is important for altering chromatin architecture which regulates access of transcriptional machinery to DNA. It plays a significant role in the control of cell growth and differentiation processes. The structural integrity of PBRM1 is necessary for the functionality of the SWI/SNF complex.

Pathways

PBRM1/BAF180 is an important player in pathways involving chromatin remodeling and transcriptional regulation. It is implicated in the WNT signaling pathway interacting with proteins such as β-catenin to influence gene transcription. It also partakes in the p53 pathway where it contributes to the regulation of cell cycle and apoptosis by modulating p53 activity.

Mutations in PBRM1 are frequently observed in renal cell carcinoma. Its disruption affects chromatin remodeling which disrupts normal gene expression contributing to cancer progression. PBRM1 also relates to breast cancer where alterations can promote tumor formation. In these contexts the interaction of PBRM1 with other proteins like VHL in renal carcinoma helps elucidate its role in tumor suppression and development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for the stability of the SWI/SNF chromatin remodeling complex SWI/SNF-B (PBAF). Acts as a negative regulator of cell proliferation.

See full target information PBRM1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biologics : targets & therapy 18:181-193 PubMed38979130

2024

STAT5B Suppresses Ferroptosis by Promoting DCAF13 Transcription to Regulate p53/xCT Pathway to Promote Mantle Cell Lymphoma Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Jun Zhang,Chong Ling Hu,Bing Ling Guo,Xi Ping Liang,Chao Yu Wang,Tao Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PBRM1/BAF180 antibody [EPR15860] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-PBRM1/BAF180 antibody [EPR15860] - BSA and Azide free (AB240338)

Immunocytochemistry/ Immunofluorescence - Anti-PBRM1/BAF180 antibody [EPR15860] - BSA and Azide free (AB240338)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBRM1/BAF180 antibody [EPR15860] - BSA and Azide free (AB240338)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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