Rabbit Recombinant Monoclonal PBRM1/BAF180 antibody. Carrier free. Suitable for WB, IP and reacts with Human samples.
Images
![Western blot - Anti-PBRM1/BAF180 antibody [EPR25199-139] - BSA and Azide free (AB305224), expandable thumbnail](https://content.abcam.com/products/images/pbrm1-baf180-antibody-epr25199-139-bsa-and-azide-free-ab305224--western-blot-img404145.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PBRM1/BAF180 antibody [EPR25199-139] - BSA and Azide free (AB305224)")
![Western blot - Anti-PBRM1/BAF180 antibody [EPR25199-139] - BSA and Azide free (AB305224), expandable thumbnail](https://content.abcam.com/products/images/pbrm1-baf180-antibody-epr25199-139-bsa-and-azide-free-ab305224--western-blot-img404144.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PBRM1/BAF180 antibody [EPR25199-139] - BSA and Azide free (AB305224)")
![Immunoprecipitation - Anti-PBRM1/BAF180 antibody [EPR25199-139] - BSA and Azide free (AB305224), expandable thumbnail](https://content.abcam.com/products/images/pbrm1-baf180-antibody-epr25199-139-bsa-and-azide-free-ab305224--immunoprecipitation-img404424.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-PBRM1/BAF180 antibody [EPR25199-139] - BSA and Azide free (AB305224)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | IP | ChIP | |
|---|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ChIP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for the stability of the SWI/SNF chromatin remodeling complex SWI/SNF-B (PBAF). Acts as a negative regulator of cell proliferation.
Alternative names
BAF180, PB1, PBRM1, Protein polybromo-1, hPB1, BRG1-associated factor 180, Polybromo-1D
Recommended products
Rabbit Recombinant Monoclonal PBRM1/BAF180 antibody. Carrier free. Suitable for WB, IP and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR25199-139
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PBRM1 also known as BAF180 is an important component of the SWI/SNF chromatin remodeling complex. It has a molecular weight of approximately 187 kDa. Within the cell PBRM1 is expressed in various tissues but shows higher expression in kidney and breast tissues. Its role involves binding to acetylated histones which influences chromatin structure and gene expression regulation.
- Biological function summary
The PBRM1/BAF180 protein functions as part of the SWI/SNF complex which includes the catalytic subunit BRG1 or BRM. This complex is important for altering chromatin architecture which regulates access of transcriptional machinery to DNA. It plays a significant role in the control of cell growth and differentiation processes. The structural integrity of PBRM1 is necessary for the functionality of the SWI/SNF complex.
- Pathways
PBRM1/BAF180 is an important player in pathways involving chromatin remodeling and transcriptional regulation. It is implicated in the WNT signaling pathway interacting with proteins such as β-catenin to influence gene transcription. It also partakes in the p53 pathway where it contributes to the regulation of cell cycle and apoptosis by modulating p53 activity.
- Associated diseases and disorders
Mutations in PBRM1 are frequently observed in renal cell carcinoma. Its disruption affects chromatin remodeling which disrupts normal gene expression contributing to cancer progression. PBRM1 also relates to breast cancer where alterations can promote tumor formation. In these contexts the interaction of PBRM1 with other proteins like VHL in renal carcinoma helps elucidate its role in tumor suppression and development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-PBRM1/BAF180 antibody [EPR25199-139] - BSA and Azide free (ab305224), expandable thumbnail](https://content.abcam.com/products/images/pbrm1-baf180-antibody-epr25199-139-bsa-and-azide-free-ab305224--western-blot-img404145.jpg "Western blot - Anti-PBRM1/BAF180 antibody [EPR25199-139] - BSA and Azide free (ab305224)")
Western blot - Anti-PBRM1/BAF180 antibody [EPR25199-139] - BSA and Azide free (ab305224)
This data was developed using Anti-PBRM1/BAF180 antibody [EPR25199-139] ab305223, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS
The identity of the bands between 30 kDa and 200 kDa are unknown.
The samples were run on a Bis-Tris gel. Performed under reducing conditions. False colour image of Western blot: Anti-PBRM1/BAF180 antibody (Anti-PBRM1/BAF180 antibody [EPR25199-139] ab305223) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-PBRM1/BAF180 antibody [EPR25199-139] ab305223 was shown to bind specifically to PBRM1/BAF180. A band was observed at 205 kDa in wild-type HAP1 cell lysates whereas no signal observed at this size in PBRM1/BAF180 knockout cell line. To generate this image, wild-type and PBRM1/BAF180 knockout HAP1 cell lysates were analyzed. First, samples were run on an SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane. Membranes were blocked in in Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.All lanes: Western blot - Anti-PBRM1/BAF180 antibody [EPR25199-139] (Anti-PBRM1/BAF180 antibody [EPR25199-139] ab305223) at 1/1000 dilution
Lane 1: Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate
Lane 2: PBRM1/BAF180 knockout HAP1 whole cell lysate
SecondaryLanes 1 - 2: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/20000 dilution
Lanes 1 - 2: Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution
Observed band size: 205 kDa
![Western blot - Anti-PBRM1/BAF180 antibody [EPR25199-139] - BSA and Azide free (ab305224), expandable thumbnail](https://content.abcam.com/products/images/pbrm1-baf180-antibody-epr25199-139-bsa-and-azide-free-ab305224--western-blot-img404144.jpg "Western blot - Anti-PBRM1/BAF180 antibody [EPR25199-139] - BSA and Azide free (ab305224)")
Western blot - Anti-PBRM1/BAF180 antibody [EPR25199-139] - BSA and Azide free (ab305224)
This data was developed using Anti-PBRM1/BAF180 antibody [EPR25199-139] ab305223, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The identity of the bands between 30 kDa and 200 kDa are unknown.
Exposure time: 158 secondsAll lanes: Western blot - Anti-PBRM1/BAF180 antibody [EPR25199-139] (Anti-PBRM1/BAF180 antibody [EPR25199-139] ab305223) at 1/1000 dilution
Lane 1: Hela (human cervical adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2: HEK-293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Observed band size: 205 kDa
Exposure time: 158s
![Immunoprecipitation - Anti-PBRM1/BAF180 antibody [EPR25199-139] - BSA and Azide free (ab305224), expandable thumbnail](https://content.abcam.com/products/images/pbrm1-baf180-antibody-epr25199-139-bsa-and-azide-free-ab305224--immunoprecipitation-img404424.jpg "Immunoprecipitation - Anti-PBRM1/BAF180 antibody [EPR25199-139] - BSA and Azide free (ab305224)")
Immunoprecipitation - Anti-PBRM1/BAF180 antibody [EPR25199-139] - BSA and Azide free (ab305224)
This data was developed using Anti-PBRM1/BAF180 antibody [EPR25199-139] ab305223, the same antibody clone in a different buffer formulation.
PBRM1/BAF180 was immunoprecipitated from 0.35 mg HAP1 (human chronic myelogenous leukemia near-haploid cell), whole cell lysate 10 µg with Anti-PBRM1/BAF180 antibody [EPR25199-139] ab305223 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-PBRM1/BAF180 antibody [EPR25199-139] ab305223 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HAP1 (human chronic myelogenous leukemia near-haploid cell), whole cell lysate 10 µg
Lane 2: Anti-PBRM1/BAF180 antibody [EPR25199-139] ab305223 IP in HAP1 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-PBRM1/BAF180 antibody [EPR25199-139] ab305223 in HAP1 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 102 secondsAll lanes: Immunoprecipitation - Anti-PBRM1/BAF180 antibody [EPR25199-139] (Anti-PBRM1/BAF180 antibody [EPR25199-139] ab305223) at 1/30 dilution
All lanes: HAP1 (human chronic myelogenous leukemia near-haploid cell)
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 205 kDa
Exposure time: 102s
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