Anti-PBX1 Antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PBX1 Antibody (AB327180)

Immunofluorescent staining of human cell line U-251 MG using ab327180 at 0.25–2 µg/ml, showing localization to nucleoplasm.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBX1 Antibody (AB327180)

Immunohistochemical staining of human fallopian tube using ab327180 at 1 : 50–1 : 200 dilution, showing strong nuclear positivity in glandular cells.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBX1 Antibody (AB327180)

Immunohistochemistry analysis in human fallopian tube and liver tissues using ab327180 at 1 : 50–1 : 200 dilution. Corresponding PBX1 RNA-seq data are presented for the same tissues.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBX1 Antibody (AB327180)

Immunohistochemical staining of human endometrium, fallopian tube, liver and prostate using ab327180 at 1 : 50–1 : 200 dilution (A) shows similar protein distribution across tissues to an independent Anti-PBX1 antibody (B).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBX1 Antibody (AB327180)

Immunohistochemical staining of human prostate using ab327180 at 1 : 50–1 : 200 dilution, showing weak to moderate nuclear positivity in glandular cells.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBX1 Antibody (AB327180)

Immunohistochemical staining of human endometrium using ab327180 at 1 : 50–1 : 200 dilution, showing moderate nuclear positivity in stromal cells.

• ChIP

Supplier Data

ChIP - Anti-PBX1 Antibody (AB327180)

ChIP-Exo-Seq composite graph for ab327180 tested in MCF7 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

• WB

Supplier Data

Western blot - Anti-PBX1 Antibody (AB327180)

Western blot analysis in human cell line NTERA-2 using ab327180 at 0.04–0.4 µg/ml.

All lanes:

Western blot - Anti-PBX1 Antibody (ab327180)

All lanes:

Human cell line NTERA-2

Predicted band size: 46.63 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBX1 Antibody (AB327180)

Immunohistochemical staining of human liver using ab327180 at 1 : 50–1 : 200 dilution, showing no positivity in hepatocytes as expected.