AB251141

Anti-PBX1/PRL antibody [EPR15937] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal PBX1/PRL antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

PRL, PBX1, Pre-B-cell leukemia transcription factor 1, Homeobox protein PBX1, Homeobox protein PRL

2 Images

Supplier Data

Immunoprecipitation - Anti-PBX1/PRL antibody [EPR15937] - BSA and Azide free (AB251141)

This data was developed using ab192606, the same antibody clone in a different buffer formulation.

Immunoprecipitation of Hela cell lysates (+) or PBS control (-) labeling PBX1 using ab192606 at 1/50 dilution and Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 as secondary.

All lanes:

Immunoprecipitation - Anti-PBX1/PRL antibody [EPR15937] (<a href='/en-us/products/primary-antibodies/pbx1-prl-antibody-epr15937-ab192606'>ab192606</a>)

Predicted band size: 29 kDa,33 kDa,47 kDa

Observed band size: 29 kDa

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Supplier Data

Western blot - Anti-PBX1/PRL antibody [EPR15937] - BSA and Azide free (AB251141)

This data was developed using ab192606, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PBX1/PRL antibody [EPR15937] (<a href='/en-us/products/primary-antibodies/pbx1-prl-antibody-epr15937-ab192606'>ab192606</a>) at 1/50000 dilution

Lane 1:

Hela cell lysate at 20 µg

Lane 2:

A431 cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated

Predicted band size: 47 kDa

Observed band size: 38 kDa

false

Unconjugated

Anti-PBX1/PRL antibody [EPR15937]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15937

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Product details

ab251141 is the carrier-free version of ab192606.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PBX1 also known as Pre-B-cell leukemia homeobox 1 is a transcription factor with a molecular mass of approximately 47 kDa. This protein belongs to the TALE family of homeodomain proteins and plays an essential role in gene regulation. PBX1 is widely expressed in many tissues including the pancreas bone marrow and certain regions of the central nervous system. It acts mainly as a DNA-binding protein that regulates the transcription of other genes by forming heterodimers with other proteins.

Biological function summary

PBX1 influences several developmental processes in vertebrates. It frequently partners with HOX proteins to form transcriptional complexes impacting the expression of genes involved in developmental pathways. These complexes assist in cellular differentiation proliferation and morphogenetic patterning. Through these roles PBX1 affects organogenesis maintaining tissue-specific gene expression and ensuring proper development of various tissues.

Pathways

PBX1 plays a part in critical developmental and signaling pathways such as the Wnt signaling pathway and the insulin signaling pathway. Within these pathways PBX1 interacts with proteins like HOXA9 and MEIS1 to regulate gene expression patterns that contribute to cellular function and intercellular communication. These interactions demonstrate the integrative role of PBX1 across single-pathway analysis impacting gene activity and cellular outcomes.

PBX1 links to the pathogenesis of acute lymphoblastic leukemia (ALL) and type 2 diabetes. In leukemia chromosomal translocations involving PBX1 and E2A (TCF3-PBX1 fusion) lead to altered cell cycle regulation and malignant transformation in hematopoietic cells. Furthermore aberrant expression of PBX1 impacts insulin signaling connecting it with the development of insulin resistance in type 2 diabetes. These interactions emphasize PBX1's importance in disease mechanisms revealing potential therapeutic targets for intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor which binds the DNA sequence 5'-TGATTGAT-3' as part of a heterodimer with HOX proteins such as HOXA1, HOXA5, HOXB7 and HOXB8 (PubMed : 9191052). Binds to the DNA sequence 5'-TGATTGAC-3' in complex with a nuclear factor which is not a class I HOX protein (PubMed : 9191052). Has also been shown to bind the DNA sequence 5'-ATCAATCAA-3' cooperatively with HOXA5, HOXB7, HOXB8, HOXC8 and HOXD4 (PubMed : 7791786, PubMed : 8327485). Acts as a transcriptional activator of PF4 in complex with MEIS1 (PubMed : 12609849). Also activates transcription of SOX3 in complex with MEIS1 by binding to the 5'-TGATTGAC-3' consensus sequence (By similarity). In natural killer cells, binds to the NFIL3 promoter and acts as a transcriptional activator of NFIL3, promoting natural killer cell development (By similarity). Plays a role in the cAMP-dependent regulation of CYP17A1 gene expression via its cAMP-regulatory sequence (CRS1) (By similarity). Probably in complex with MEIS2, involved in transcriptional regulation by KLF4 (PubMed : 21746878). Acts as a transcriptional activator of NKX2-5 and a transcriptional repressor of CDKN2B (By similarity). Together with NKX2-5, required for spleen development through a mechanism that involves CDKN2B repression (By similarity).. Isoform PBX1b. As part of a PDX1 : PBX1b : MEIS2B complex in pancreatic acinar cells, is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element.

See full target information PBX1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 22: PubMed34769092

2021

11,12 Epoxyeicosatrienoic Acid Rescues Deteriorated Wound Healing in Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina Sommer,Heike Jakob,Caroline Reiche,Dirk Henrich,Jasmina Sterz,Johannes Frank,Ingo Marzi,Anna Lena Sander

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PBX1/PRL antibody [EPR15937] - BSA and Azide free

Immunoprecipitation - Anti-PBX1/PRL antibody [EPR15937] - BSA and Azide free (AB251141)

Western blot - Anti-PBX1/PRL antibody [EPR15937] - BSA and Azide free (AB251141)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise