Rabbit Polyclonal PC1/3 antibody. Suitable for IHC-P, WB and reacts with Rat, Human samples. Immunogen corresponding to Synthetic Peptide within Human PCSK1 aa 700-750.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human PCSK1 aa 700-750. The exact immunogen used to generate this antibody is proprietary information. Database link P29120
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Predicted | Predicted |
| Rat | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 0.50000-1.00000 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 0.50000-1.00000 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 0.10000-0.50000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin, insulin and AGRP.
Alternative names
Neuroendocrine convertase 1, NEC 1, Prohormone convertase 1, Proprotein convertase 1, PC1, NEC1, PCSK1
Recommended products
Rabbit Polyclonal PC1/3 antibody. Suitable for IHC-P, WB and reacts with Rat, Human samples. Immunogen corresponding to Synthetic Peptide within Human PCSK1 aa 700-750.
Alternative names
Neuroendocrine convertase 1, NEC 1, Prohormone convertase 1, Proprotein convertase 1, PC1, NEC1, PCSK1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human PCSK1 aa 700-750. The exact immunogen used to generate this antibody is proprietary information. Database link P29120
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PC1/3 also known as Proprotein Convertase 1 or PCSK1 is a member of the proprotein convertase family which is responsible for processing precursor proteins into their active forms. This protein with a mass of approximately 84 kDa is expressed in neuroendocrine and endocrine tissues. PC1/3 operates primarily in the secretory pathways of these tissues cleaving prohormones and other precursor proteins at specific sites to generate active peptides and proteins essential for various physiological processes.
- Biological function summary
The proprotein convertase enzyme PC1/3 plays a critical role in activating hormone precursors. It is involved in the conversion of proinsulin to insulin as well as the processing of proopiomelanocortin (POMC) to generate peptides like ACTH and beta-endorphin. PC1/3 does not generally form part of a complex but interacts closely with other convertases for complete prohormone processing. This enzyme is essential for maintaining homeostasis in hormone regulation and is an important player in metabolic control.
- Pathways
Specific functions of PC1/3 are closely tied to metabolic and neuroendocrine pathways. It is significant in the insulin signaling pathway where its role in converting proinsulin to insulin directly impacts glucose metabolism. Additionally PC1/3 contributes to neuropeptide signaling pathways by processing neuroendocrine peptides which influence appetite and energy homeostasis. PC1/3 works alongside proteins like PC2 another proprotein convertase to ensure comprehensive processing of peptide hormones.
- Associated diseases and disorders
Malfunction or deficiency in PC1/3 activity is associated with disorders such as obesity and hypogonadotropic hypogonadism. Obesity can result from improper proinsulin processing affecting insulin regulation. Hypogonadotropic hypogonadism may occur due to faulty prohormone processing impacting gonadotropin-releasing hormone production. PC1/3 interactions with other convertases like PC2 highlight its involvement in regulatory networks that maintain metabolic and reproductive health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC1/3 antibody (ab191452)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of PC1/3 using ab191452 in human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL ab191452 overnight at 4°C. DyLight®488 Conjugated Avidin was used and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC1/3 antibody (ab191452)
Immunohistochemical analysis of formalin-fixed paraffin-embedded Rat Intestine tissue labeling PC1/3 with ab191452 at 1 μg/ml.
Western blot - Anti-PC1/3 antibody (ab191452)
All lanes: Western blot - Anti-PC1/3 antibody (ab191452) at 0.5 µg/mL
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Thymus Tissue Lysate
Lane 3: A549 cell lysate
Lane 4: HELA cell lysate
Lane 5: COLO320 cell lysate
Lane 6: PANC cell lysate
Predicted band size: 84 kDa
Downloads
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