AB3532

Anti-PC1/3 antibody

Name: Anti-PC1/3 antibody (ab3532) | Abcam
Brand: Abcam Limited
SKU: AB3532
Rating: 3 (1 reviews)

(1 Review)

(6 Publications)

Rabbit Polyclonal PC1/3 antibody. Suitable for WB and reacts with Mouse samples. Cited in 6 publications.

View Alternative Names

Att-1, Nec-1, Nec1, Pcsk1, Neuroendocrine convertase 1, NEC 1, Furin homolog, PC3, Prohormone convertase 1, Propeptide-processing protease, Proprotein convertase 1, PC1

2 Images

Unknown

Western blot - Anti-PC1/3 antibody (AB3532)

PC1/3 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The band observed at 68 kDa could potentially be a cleaved form of PC1/3 due to the presence of a 27 amino acid signal peptide and an 83 amino acid propeptide.

All lanes:

Western blot - Anti-PC1/3 antibody (ab3532) at 1 µg/mL

All lanes:

Spinal Cord (Mouse) Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 84 kDa

Observed band size: 35 kDa,60 kDa,68-87 kDa

true

Exposure time: 4min

Unknown

Western blot - Anti-PC1/3 antibody (AB3532)

Western blot detection of PC1/3 from mouse pituitary extract using ab3532.

All lanes:

Western blot - Anti-PC1/3 antibody (ab3532)

Predicted band size: 84 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PC1/3 also known as Proprotein Convertase 1 or PCSK1 is a member of the proprotein convertase family which is responsible for processing precursor proteins into their active forms. This protein with a mass of approximately 84 kDa is expressed in neuroendocrine and endocrine tissues. PC1/3 operates primarily in the secretory pathways of these tissues cleaving prohormones and other precursor proteins at specific sites to generate active peptides and proteins essential for various physiological processes.

Biological function summary

The proprotein convertase enzyme PC1/3 plays a critical role in activating hormone precursors. It is involved in the conversion of proinsulin to insulin as well as the processing of proopiomelanocortin (POMC) to generate peptides like ACTH and beta-endorphin. PC1/3 does not generally form part of a complex but interacts closely with other convertases for complete prohormone processing. This enzyme is essential for maintaining homeostasis in hormone regulation and is an important player in metabolic control.

Pathways

Specific functions of PC1/3 are closely tied to metabolic and neuroendocrine pathways. It is significant in the insulin signaling pathway where its role in converting proinsulin to insulin directly impacts glucose metabolism. Additionally PC1/3 contributes to neuropeptide signaling pathways by processing neuroendocrine peptides which influence appetite and energy homeostasis. PC1/3 works alongside proteins like PC2 another proprotein convertase to ensure comprehensive processing of peptide hormones.

Malfunction or deficiency in PC1/3 activity is associated with disorders such as obesity and hypogonadotropic hypogonadism. Obesity can result from improper proinsulin processing affecting insulin regulation. Hypogonadotropic hypogonadism may occur due to faulty prohormone processing impacting gonadotropin-releasing hormone production. PC1/3 interactions with other convertases like PC2 highlight its involvement in regulatory networks that maintain metabolic and reproductive health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin, insulin and AGRP.

See full target information Pcsk1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

eLife 11: PubMed36469459

2022

Neuroendocrinology of the lung revealed by single-cell RNA sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Christin S Kuo,Spyros Darmanis,Alex Diaz de Arce,Yin Liu,Nicole Almanzar,Timothy Ting-Hsuan Wu,Stephen R Quake,Mark A Krasnow

Translational neurodegeneration 10:37 PubMed34565482

2021

Dense core vesicle markers in CSF and cortical tissues of patients with Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Neus Barranco,Virginia Plá,Daniel Alcolea,Irene Sánchez-Domínguez,Reiner Fischer-Colbrie,Isidro Ferrer,Alberto Lleó,Fernando Aguado

JCI insight 6: PubMed33554958

2021

GLP-1 receptor signaling increases PCSK1 and β cell features in human α cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mridusmita Saikia,Marlena M Holter,Leanne R Donahue,Isaac S Lee,Qiaonan C Zheng,Journey L Wise,Jenna E Todero,Daryl J Phuong,Darline Garibay,Reilly Coch,Kyle W Sloop,Adolfo Garcia-Ocana,Charles G Danko,Bethany P Cummings

Neuroendocrinology 110:92-104 PubMed31104058

2019

Proopiomelanocortin Processing in the Hypothalamus Is Directly Regulated by Saturated Fat: Implications for the Development of Obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Daniela S Razolli,Thiago M de Araújo,Marcella R Sant Apos Ana,Peter Kirwan,Dennys E Cintra,Florian T Merkle,Licio A Velloso

Biochemical pharmacology 85:81-91 PubMed23085436

2012

Chemical cholecystokinin receptor activation protects against obesity-diabetes in high fat fed mice and has sustainable beneficial effects in genetic ob/ob mice.

Applications

Unspecified application

Species

Unspecified reactive species

Nigel Irwin,Ian A Montgomery,R Charlotte Moffett,Peter R Flatt

Regulatory peptides 152:54-60 PubMed18706454

2008

Expression of PCSK1 (PC1/3), PCSK2 (PC2) and PCSK3 (furin) in mouse small intestine.

Applications

Unspecified application

Species

Unspecified reactive species

Jeffrey Gagnon,Janice Mayne,Majambu Mbikay,John Woulfe,Michel Chrétien

View all publications

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Product promise

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Anti-PC1/3 antibody

Western blot - Anti-PC1/3 antibody (AB3532)

Western blot - Anti-PC1/3 antibody (AB3532)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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