Anti-PC1/3 antibody [EPR21908] Rabbit monoclonal (ab220363)

Rabbit Recombinant Monoclonal PC1/3 antibody. Suitable for IHC-P, IP, WB, ICC/IF, IHC-Fr, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

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Images

Western blot - Anti-PC1/3 antibody [EPR21908] (AB220363), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IP	WB	ICC/IF	IHC-Fr	Flow Cyt (Intra)
Human	Tested	Expected	Expected	Expected	Expected	Expected
Mouse	Tested	Tested	Tested	Tested	Tested	Tested
Rat	Tested	Expected	Expected	Expected	Tested	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Human	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes -
Species Rat	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/50	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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2 products for Alternative Product

Target data

See full target information PCSK1

Function

Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin, insulin and AGRP.

Alternative names

NEC1, PCSK1, Neuroendocrine convertase 1, NEC 1, Prohormone convertase 1, Proprotein convertase 1, PC1

Recommended products

Rabbit Recombinant Monoclonal PC1/3 antibody. Suitable for IHC-P, IP, WB, ICC/IF, IHC-Fr, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR21908
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PC1/3 also known as Proprotein Convertase 1 or PCSK1 is a member of the proprotein convertase family which is responsible for processing precursor proteins into their active forms. This protein with a mass of approximately 84 kDa is expressed in neuroendocrine and endocrine tissues. PC1/3 operates primarily in the secretory pathways of these tissues cleaving prohormones and other precursor proteins at specific sites to generate active peptides and proteins essential for various physiological processes.

Biological function summary

The proprotein convertase enzyme PC1/3 plays a critical role in activating hormone precursors. It is involved in the conversion of proinsulin to insulin as well as the processing of proopiomelanocortin (POMC) to generate peptides like ACTH and beta-endorphin. PC1/3 does not generally form part of a complex but interacts closely with other convertases for complete prohormone processing. This enzyme is essential for maintaining homeostasis in hormone regulation and is an important player in metabolic control.

Pathways

Specific functions of PC1/3 are closely tied to metabolic and neuroendocrine pathways. It is significant in the insulin signaling pathway where its role in converting proinsulin to insulin directly impacts glucose metabolism. Additionally PC1/3 contributes to neuropeptide signaling pathways by processing neuroendocrine peptides which influence appetite and energy homeostasis. PC1/3 works alongside proteins like PC2 another proprotein convertase to ensure comprehensive processing of peptide hormones.

Associated diseases and disorders

Malfunction or deficiency in PC1/3 activity is associated with disorders such as obesity and hypogonadotropic hypogonadism. Obesity can result from improper proinsulin processing affecting insulin regulation. Hypogonadotropic hypogonadism may occur due to faulty prohormone processing impacting gonadotropin-releasing hormone production. PC1/3 interactions with other convertases like PC2 highlight its involvement in regulatory networks that maintain metabolic and reproductive health.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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12 product images

Western blot - Anti-PC1/3 antibody [EPR21908] (ab220363)
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
The PC1/3 protein undergoes multiple intracellular cleavage steps to its 66 kDa mature form; the 87 kDa represents the ProPC1/3 intermediate (PMID: 26778167).
This blot was developed using a higher sensitivity ECL substrate.
Negative control: NIH/3T3 (PMID: 9405066; PMID:15143067).
All lanes: Western blot - Anti-PC1/3 antibody [EPR21908] (ab220363) at 1/1000 dilution
Lane 1: Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate at 10 µg
Lane 2: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 84 kDa
Exposure time: 125s
Immunohistochemistry (Frozen sections) - Anti-PC1/3 antibody [EPR21908] (ab220363)
Immunohistochemical analysis of frozen rat pancreas tissue labeling PC1/3 with ab220363 at 1/500 dilution (green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive cytoplasmic staining in rat pancreatic islet (PMID: 25976560) is observed. Counter stained with DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1000 dilution.
Perform heat-mediated antigen retrieval by using Tris-EDTA buffer (10mM Tris base pH 9.0, 1mM EDTA, 0.05% Tween 20).
Western blot - Anti-PC1/3 antibody [EPR21908] (ab220363)
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
The 66 kDa band is the mature form of PC1/3 (PMID: 26778167).
This blot was developed using a higher sensitivity ECL substrate.
All lanes: Western blot - Anti-PC1/3 antibody [EPR21908] (ab220363) at 1/1000 dilution
All lanes: Mouse hypothalamus lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 84 kDa
Exposure time: 3min
Immunohistochemistry (Frozen sections) - Anti-PC1/3 antibody [EPR21908] (ab220363)
Immunohistochemical analysis of frozen mouse pancreas tissue labeling PC1/3 with ab220363 at 1/500 dilution (green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive cytoplasmic staining in mouse pancreatic islet (PMID: 25976560) is observed. Counter stained with DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1000 dilution.
Perform heat-mediated antigen retrieval by using Tris-EDTA buffer (10mM Tris base pH 9.0, 1mM EDTA, 0.05% Tween 20).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC1/3 antibody [EPR21908] (ab220363)
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling PC1/3 with ab220363 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in rat pancreatic islets (PMID: 7925129; PMID: 21190012) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat-mediated antigen retrieval using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC1/3 antibody [EPR21908] (ab220363)
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling PC1/3 with ab220363 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in mouse pancreatic islets (PMID: 7925129; PMID: 21190012) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat-mediated antigen retrieval using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC1/3 antibody [EPR21908] (ab220363)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling PC1/3 with ab220363 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Sporadic cytoplasmic staining (arrows) in human colon (PMID: 18706454) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat-mediated antigen retrieval using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0).
Immunoprecipitation - Anti-PC1/3 antibody [EPR21908] (ab220363)
PC1/3 was immunoprecipitated from 0.35 mg Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate with ab220363 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220363 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.
Lane 1: Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate 10 μg (Input).
Lane 2: ab220363 IP in Beta-TC-6 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab220363 in Beta-TC-6 whole cell lysate (-).

Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
The PC1/3 protein undergoes multiple intracellular cleavage steps to its 66 kDa mature form (PMID: 26778167).
All lanes: Immunoprecipitation - Anti-PC1/3 antibody [EPR21908] (ab220363)
Predicted band size: 84 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC1/3 antibody [EPR21908] (ab220363)
Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling PC1/3 with ab220363 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in human pancreatic islets (PMID: 7925129; PMID: 21190012) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat-mediated antigen retrieval using Antigen Retrieval Buffer (100X Citrate Buffer) ab93678 (citrate buffer, pH 6.0).
Immunocytochemistry/ Immunofluorescence - Anti-PC1/3 antibody [EPR21908] (ab220363)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Beta-TC-6 (mouse pancreas insulinoma beta cell) cells labeling PC1/3 with ab220363 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining in Beta-TC-6 cell line.
Negative cell control: NIH/3T3 (PMID: 9405066; PMID:15143067).
Counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at a 1/200 dilution (red). The nuclear counter stain is DAPI (blue). The negative control is the secondary antibody only.
Flow Cytometry (Intracellular) - Anti-PC1/3 antibody [EPR21908] (ab220363)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Beta-TC-6 (mouse pancreas insulinoma beta cell) cell line labeling PC1/3 with ab220363 at 1/50 (red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-PC1/3 antibody [EPR21908] (ab220363)
Blocking and Diluting Buffer: 5% NFDM /TBST

87 kDa ProPC1/3; 66 kDa mature form (PMID: 26778167)

Lane1 Expsoure Time: 20 seconds

Lane2 and Lane3 were developed using a high sensitivity ECL substrate.
All lanes: Western blot - Anti-PC1/3 antibody [EPR21908] (ab220363) at 1/1000 dilution
Lane 1: Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate at 20 µg
Lane 2: Mouse pancreas lysate at 20 µg
Lane 3: Mouse brain lysate at 20 µg
Secondary
All lanes: Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (PE Anti-ENPP3/B10 antibody [NP4D6] ab90751) at 1/20000 dilution
Predicted band size: 87 kDa
Observed band size: 66 kDa
Exposure time: 80s