Anti-PC1/3 antibody [EPR21908]
- RabMAb
- Recombinant
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(10 Publications)
Rabbit Recombinant Monoclonal PC1/3 antibody. Suitable for IHC-P, IP, WB, ICC/IF, IHC-Fr, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 10 publications.
View Alternative Names
NEC1, PCSK1, Neuroendocrine convertase 1, NEC 1, Prohormone convertase 1, Proprotein convertase 1, PC1
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC1/3 antibody [EPR21908] (AB220363)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling PC1/3 with ab220363 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Sporadic cytoplasmic staining (arrows) in human colon (PMID : 18706454) is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat-mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC1/3 antibody [EPR21908] (AB220363)
Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling PC1/3 with ab220363 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in human pancreatic islets (PMID : 7925129; PMID : 21190012) is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat-mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-PC1/3 antibody [EPR21908] (AB220363)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Beta-TC-6 (mouse pancreas insulinoma beta cell) cells labeling PC1/3 with ab220363 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining in Beta-TC-6 cell line.
Negative cell control : NIH/3T3 (PMID : 9405066; PMID : 15143067).
Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red). The nuclear counter stain is DAPI (blue). The negative control is the secondary antibody only.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC1/3 antibody [EPR21908] (AB220363)
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling PC1/3 with ab220363 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in rat pancreatic islets (PMID : 7925129; PMID : 21190012) is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat-mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
- IHC-Fr
Supplier Data
Immunohistochemistry (Frozen sections) - Anti-PC1/3 antibody [EPR21908] (AB220363)
Immunohistochemical analysis of frozen mouse pancreas tissue labeling PC1/3 with ab220363 at 1/500 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive cytoplasmic staining in mouse pancreatic islet (PMID : 25976560) is observed. Counter stained with DAPI (blue).
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1000 dilution.
Perform heat-mediated antigen retrieval by using Tris-EDTA buffer (10mM Tris base pH 9.0, 1mM EDTA, 0.05% Tween 20).
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-PC1/3 antibody [EPR21908] (AB220363)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Beta-TC-6 (mouse pancreas insulinoma beta cell) cell line labeling PC1/3 with ab220363 at 1/50 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC1/3 antibody [EPR21908] (AB220363)
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling PC1/3 with ab220363 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in mouse pancreatic islets (PMID : 7925129; PMID : 21190012) is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat-mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
- IHC-Fr
Supplier Data
Immunohistochemistry (Frozen sections) - Anti-PC1/3 antibody [EPR21908] (AB220363)
Immunohistochemical analysis of frozen rat pancreas tissue labeling PC1/3 with ab220363 at 1/500 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive cytoplasmic staining in rat pancreatic islet (PMID : 25976560) is observed. Counter stained with DAPI (blue).
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1000 dilution.
Perform heat-mediated antigen retrieval by using Tris-EDTA buffer (10mM Tris base pH 9.0, 1mM EDTA, 0.05% Tween 20).
- IP
Supplier Data
Immunoprecipitation - Anti-PC1/3 antibody [EPR21908] (AB220363)
PC1/3 was immunoprecipitated from 0.35 mg Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate with ab220363 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220363 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1 : Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate 10 μg (Input).
Lane 2 : ab220363 IP in Beta-TC-6 whole cell lysate (+).
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab220363 in Beta-TC-6 whole cell lysate (-).
Blocking/Dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 minutes.
The PC1/3 protein undergoes multiple intracellular cleavage steps to its 66 kDa mature form (PMID : 26778167).
All lanes:
Immunoprecipitation - Anti-PC1/3 antibody [EPR21908] (ab220363)
Predicted band size: 84 kDa
false
- WB
Supplier Data
Western blot - Anti-PC1/3 antibody [EPR21908] (AB220363)
Blocking/Dilution buffer and concentration : 5% NFDM/TBST.
The PC1/3 protein undergoes multiple intracellular cleavage steps to its 66 kDa mature form; the 87 kDa represents the ProPC1/3 intermediate (PMID : 26778167).
This blot was developed using a higher sensitivity ECL substrate.
Negative control : NIH/3T3 (PMID : 9405066; PMID : 15143067).
All lanes:
Western blot - Anti-PC1/3 antibody [EPR21908] (ab220363) at 1/1000 dilution
Lane 1:
Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate at 10 µg
Lane 2:
NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 84 kDa
true
Exposure time: 125s
- WB
Supplier Data
Western blot - Anti-PC1/3 antibody [EPR21908] (AB220363)
Blocking and Diluting Buffer : 5% NFDM /TBST 87 kDa ProPC1/3; 66 kDa mature form (PMID : 26778167) Lane1 Expsoure Time : 20 seconds Lane2 and Lane3 were developed using a high sensitivity ECL substrate.
All lanes:
Western blot - Anti-PC1/3 antibody [EPR21908] (ab220363) at 1/1000 dilution
Lane 1:
Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate at 20 µg
Lane 2:
Mouse pancreas lysate at 20 µg
Lane 3:
Mouse brain lysate at 20 µg
Secondary
All lanes:
Western blot - PE Anti-ENPP3/B10 antibody [NP4D6] (<a href='/en-us/products/primary-antibodies/pe-enpp3-b10-antibody-np4d6-ab90751'>ab90751</a>) at 1/20000 dilution
Predicted band size: 87 kDa
Observed band size: 66 kDa
false
Exposure time: 80s
- WB
Supplier Data
Western blot - Anti-PC1/3 antibody [EPR21908] (AB220363)
Blocking/Dilution buffer and concentration : 5% NFDM/TBST.
The 66 kDa band is the mature form of PC1/3 (PMID : 26778167).
This blot was developed using a higher sensitivity ECL substrate.
All lanes:
Western blot - Anti-PC1/3 antibody [EPR21908] (ab220363) at 1/1000 dilution
All lanes:
Mouse hypothalamus lysate at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 84 kDa
true
Exposure time: 3min
Related conjugates and formulations (1)
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Anti-PC1/3 antibody [EPR21908] - BSA and Azide free
Reactivity data
Product details
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The proprotein convertase enzyme PC1/3 plays a critical role in activating hormone precursors. It is involved in the conversion of proinsulin to insulin as well as the processing of proopiomelanocortin (POMC) to generate peptides like ACTH and beta-endorphin. PC1/3 does not generally form part of a complex but interacts closely with other convertases for complete prohormone processing. This enzyme is essential for maintaining homeostasis in hormone regulation and is an important player in metabolic control.
Pathways
Specific functions of PC1/3 are closely tied to metabolic and neuroendocrine pathways. It is significant in the insulin signaling pathway where its role in converting proinsulin to insulin directly impacts glucose metabolism. Additionally PC1/3 contributes to neuropeptide signaling pathways by processing neuroendocrine peptides which influence appetite and energy homeostasis. PC1/3 works alongside proteins like PC2 another proprotein convertase to ensure comprehensive processing of peptide hormones.
Product protocols
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Target data
Publications (10)
Recent publications for all applications. Explore the full list and refine your search
Probiotics and antimicrobial proteins : PubMed40366615
2025
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Diabetes, obesity & metabolism 27:1296-1313 PubMed39803714
2025
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Experimental & molecular medicine 57:131-150 PubMed39741186
2025
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iScience 27:110017 PubMed39021804
2024
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Cell metabolism 36:48-61.e6 PubMed38128529
2023
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Frontiers in pharmacology 14:1228722 PubMed37469873
2023
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Stem cell research & therapy 14:45 PubMed36941658
2023
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The Journal of cell biology 221: PubMed36053215
2022
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eLife 9: PubMed33164744
2020
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Diabetes, metabolic syndrome and obesity : targets 13:1407-1415 PubMed32425572
2020
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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