Rabbit Polyclonal PC4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SUB1 aa 1 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SUB1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P53999
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Cynomolgus monkey | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cynomolgus monkey, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cynomolgus monkey, Orangutan | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cynomolgus monkey, Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA).
Alternative names
PC4, RPO2TC1, SUB1, Activated RNA polymerase II transcriptional coactivator p15, Positive cofactor 4, SUB1 homolog, p14
Recommended products
Rabbit Polyclonal PC4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SUB1 aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SUB1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P53999
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PC4 also known as Sub1 is a transcriptional coactivator protein. Its molecular weight is approximately 20 kDa. The protein is expressed in various tissues with particularly high expression in the liver and heart. PC4 facilitates transcription by interacting with basal transcription factors. It bonds with single-stranded DNA enhancing RNA polymerase II's ability to start transcription.
- Biological function summary
Many experts note PC4's significant role in transcriptional regulation and chromatin structure dynamics. It operates as part of a multifunctional complex that regulates gene expression. PC4 stabilizes transcriptional pre-initiation complexes which helps initiate gene transcription. Its capacity to modulate chromatin can also impact DNA repair and replication processes emphasizing its diverse roles beyond transcriptional activation.
- Pathways
Transcription regulation processes often feature PC4 prominently. It interacts within the transcriptional machinery alongside proteins such as TFIIB and TFIIH. Additionally PC4 participates in chromatin remodeling pathways which affect DNA accessibility and gene transcription. This protein's interaction with other transcription factors establishes it as a versatile component in gene expression pathways.
- Associated diseases and disorders
Researchers link PC4 to cancer and cardiac hypertrophy. In cancer PC4 can influence oncogene activation and tumor suppressor pathways interacting with proteins like p53 in these contexts. Studies also suggest PC4 involvement in cardiac hypertrophy by affecting genes associated with heart muscle enlargement where it functions alongside other cardiac stress-responsive proteins. These associations highlight PC4's potential as a therapeutic target in various pathologies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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6 product images
Immunocytochemistry/ Immunofluorescence - Anti-PC4 antibody (ab244248)
PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling PC4 using ab244248 at 4 μg/ml (green) in ICC/IF.
Western blot - Anti-PC4 antibody (ab244248)
All lanes: Western blot - Anti-PC4 antibody (ab244248) at 0.4 µg/mL
All lanes: RPMI-8226 (Human cell line) cell lysate
Predicted band size: 14 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC4 antibody (ab244248)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for PC4 with ab244248 at a 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC4 antibody (ab244248)
Formalin-fixed, paraffin-embedded human cerebellum tissue stained for PC4 with ab244248 at a 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC4 antibody (ab244248)
Formalin-fixed, paraffin-embedded human rectum tissue stained for PC4 with ab244248 at a 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC4 antibody (ab244248)
Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for PC4 with ab244248 at a 1/200 dilution in immunohistochemical analysis.
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