Rabbit Polyclonal PC4 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human SUB1.
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Publications
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- Journal of oncology 2021:99584832021The Human Positive Cofactor 4 is a Promising Chemotherapeutic Target in Lung Adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTianyu Sun,Jingge Zhang,Xiaoqing Fan,Tan Long,Shaolin Tao,Poming Kang,Qunyou TanPubMed 34899911
- BMC molecular biology 19:92018Positive cofactor 4 (PC4) contributes to the regulation of replication-dependent canonical histone gene expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAleksandra Brzek et. al.PubMed 30053800
- Nucleic acids research 46:293-3132017Translation can affect the antisense activity of RNase H1-dependent oligonucleotides targeting mRNAs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXue-Hai Liang,Joshua G Nichols,Hong Sun,Stanley T CrookePubMed 29165591
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SUB1. The exact immunogen used to generate this antibody is proprietary information. Database link P53999
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Chimpanzee | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Elephant | Predicted | Predicted | Predicted |
| Guinea pig | Predicted | Predicted | Predicted |
| Horse | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Orangutan, Elephant | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Orangutan, Elephant | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Orangutan, Elephant | Dilution info - | Notes - |
Associated Products
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Target data
Function
General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA).
Alternative names
PC4, RPO2TC1, SUB1, Activated RNA polymerase II transcriptional coactivator p15, Positive cofactor 4, SUB1 homolog, p14
Recommended products
Rabbit Polyclonal PC4 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human SUB1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SUB1. The exact immunogen used to generate this antibody is proprietary information. Database link P53999
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PC4 also known as Sub1 is a transcriptional coactivator protein. Its molecular weight is approximately 20 kDa. The protein is expressed in various tissues with particularly high expression in the liver and heart. PC4 facilitates transcription by interacting with basal transcription factors. It bonds with single-stranded DNA enhancing RNA polymerase II's ability to start transcription.
- Biological function summary
Many experts note PC4's significant role in transcriptional regulation and chromatin structure dynamics. It operates as part of a multifunctional complex that regulates gene expression. PC4 stabilizes transcriptional pre-initiation complexes which helps initiate gene transcription. Its capacity to modulate chromatin can also impact DNA repair and replication processes emphasizing its diverse roles beyond transcriptional activation.
- Pathways
Transcription regulation processes often feature PC4 prominently. It interacts within the transcriptional machinery alongside proteins such as TFIIB and TFIIH. Additionally PC4 participates in chromatin remodeling pathways which affect DNA accessibility and gene transcription. This protein's interaction with other transcription factors establishes it as a versatile component in gene expression pathways.
- Associated diseases and disorders
Researchers link PC4 to cancer and cardiac hypertrophy. In cancer PC4 can influence oncogene activation and tumor suppressor pathways interacting with proteins like p53 in these contexts. Studies also suggest PC4 involvement in cardiac hypertrophy by affecting genes associated with heart muscle enlargement where it functions alongside other cardiac stress-responsive proteins. These associations highlight PC4's potential as a therapeutic target in various pathologies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-PC4 antibody (ab72132)
All lanes: Western blot - Anti-PC4 antibody (ab72132) at 0.04 µg/mL
Lane 1: Whole cell lysate from Hela cells at 50 µg
Lane 2: Whole cell lysate from Hela cells at 15 µg
Lane 3: Whole cell lysate from Hela cells at 5 µg
Predicted band size: 14 kDa
Observed band size: 18 kDa, 190 kDa
Immunoprecipitation - Anti-PC4 antibody (ab72132)
Immunoprecipitation/ Western Blot of PC4.
Lane 1: ab72132 at 3μg/mg whole cell lysate.
Lane 2: Control IgG.
ab72132 at 0.1μg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Detection:Chemiluminescence with an exposure time of 30 seconds.All lanes: Immunoprecipitation - Anti-PC4 antibody (ab72132)
Predicted band size: 14 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC4 antibody (ab72132)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling PC4 with ab72132 at 1/200 (1µg/ml). Detection: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PC4 antibody (ab72132)
IHC image of ab72132 staining in human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72132, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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