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Rabbit Recombinant Monoclonal PC4 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

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Images

Western blot - Anti-PC4 antibody [EPR9281] (AB154852), expandable thumbnail
  • Immunoprecipitation - Anti-PC4 antibody [EPR9281] (AB154852), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PC4 antibody [EPR9281] (AB154852), expandable thumbnail
  • Western blot - Anti-PC4 antibody [EPR9281] (AB154852), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PC4 antibody [EPR9281] (AB154852), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IF
Human
Tested
Tested
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

For unpurified, use 1/100 - 1/250.

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA).

Alternative names

PC4, RPO2TC1, SUB1, Activated RNA polymerase II transcriptional coactivator p15, Positive cofactor 4, SUB1 homolog, p14

Recommended products

Rabbit Recombinant Monoclonal PC4 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR9281
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PC4 also known as Sub1 is a transcriptional coactivator protein. Its molecular weight is approximately 20 kDa. The protein is expressed in various tissues with particularly high expression in the liver and heart. PC4 facilitates transcription by interacting with basal transcription factors. It bonds with single-stranded DNA enhancing RNA polymerase II's ability to start transcription.

Biological function summary

Many experts note PC4's significant role in transcriptional regulation and chromatin structure dynamics. It operates as part of a multifunctional complex that regulates gene expression. PC4 stabilizes transcriptional pre-initiation complexes which helps initiate gene transcription. Its capacity to modulate chromatin can also impact DNA repair and replication processes emphasizing its diverse roles beyond transcriptional activation.

Pathways

Transcription regulation processes often feature PC4 prominently. It interacts within the transcriptional machinery alongside proteins such as TFIIB and TFIIH. Additionally PC4 participates in chromatin remodeling pathways which affect DNA accessibility and gene transcription. This protein's interaction with other transcription factors establishes it as a versatile component in gene expression pathways.

Associated diseases and disorders

Researchers link PC4 to cancer and cardiac hypertrophy. In cancer PC4 can influence oncogene activation and tumor suppressor pathways interacting with proteins like p53 in these contexts. Studies also suggest PC4 involvement in cardiac hypertrophy by affecting genes associated with heart muscle enlargement where it functions alongside other cardiac stress-responsive proteins. These associations highlight PC4's potential as a therapeutic target in various pathologies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-PC4 antibody [EPR9281] (ab154852), expandable thumbnail

    Western blot - Anti-PC4 antibody [EPR9281] (ab154852)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-PC4 antibody [EPR9281] (ab154852) at 1/5000 dilution

    Lane 1: MCF7 cell lysate at 20 µg

    Lane 2: T-47D cell lysate at 20 µg

    Lane 3: PC-3 cell lysate at 20 µg

    Lane 4: Jurkat cell lysate at 20 µg

    Secondary

    All lanes: HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size: 14 kDa

    Observed band size: 14 kDa

  • Immunoprecipitation - Anti-PC4 antibody [EPR9281] (ab154852), expandable thumbnail

    Immunoprecipitation - Anti-PC4 antibody [EPR9281] (ab154852)

    ab154852 (purified) at 1/70 immunoprecipitating PC4 in 10 μg Jurkat (Lanes 1 and 2, observed at 14 kDa). Lane 3 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

    All lanes: Immunoprecipitation - Anti-PC4 antibody [EPR9281] (ab154852)

    Predicted band size: 14 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PC4 antibody [EPR9281] (ab154852), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PC4 antibody [EPR9281] (ab154852)

    Immunofluorescence staining of T47D cells with purified ab154852 at a working dilution of 1/800, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), used at a dilution of 1/1000. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab154852 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at a dilution of 1/500. For negative control 2, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at a dilution of 1/400.

  • Western blot - Anti-PC4 antibody [EPR9281] (ab154852), expandable thumbnail

    Western blot - Anti-PC4 antibody [EPR9281] (ab154852)

    All lanes: Western blot - Anti-PC4 antibody [EPR9281] (ab154852) at 1/1000 dilution

    Lane 1: MCF7 cell lysate at 10 µg

    Lane 2: T47D cell lysate at 10 µg

    Lane 3: Jurkat cell lysate at 10 µg

    Lane 4: PC3 cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 14 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PC4 antibody [EPR9281] (ab154852), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PC4 antibody [EPR9281] (ab154852)

    Immunofluorescent analysis of MCF7 cells labeling PC4 with unpurified ab154852 at 1/100.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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