Anti-PC4 antibody [EPR9281] - BSA and Azide free

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PC4 antibody [EPR9281] - BSA and Azide free (AB249154)

This data was developed using ab154852, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of MCF7 cells labeling PC4 with unpurified ab154852 at 1/100.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PC4 antibody [EPR9281] - BSA and Azide free (AB249154)

This data was developed using ab154852, the same antibody clone in a different buffer formulation.

Immunofluorescence staining of T47D cells with purified ab154852 at a working dilution of 1/800, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor^® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab154852 was used at a dilution of 1/500 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor^® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

• IP

Unknown

Immunoprecipitation - Anti-PC4 antibody [EPR9281] - BSA and Azide free (AB249154)

This data was developed using ab154852, the same antibody clone in a different buffer formulation.ab154852 (purified) at 1/70 immunoprecipitating PC4 in 10 µg Jurkat (Lanes 1 and 2, observed at 14 kDa). Lane 3 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500). Blocking buffer and concentration : 5% NFDM/TBST Dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-PC4 antibody [EPR9281] (<a href='/en-us/products/primary-antibodies/pc4-antibody-epr9281-ab154852'>ab154852</a>)

Predicted band size: 14 kDa

false

• WB

Lab

Western blot - Anti-PC4 antibody [EPR9281] - BSA and Azide free (AB249154)

This data was developed using ab154852, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PC4 antibody [EPR9281] (<a href='/en-us/products/primary-antibodies/pc4-antibody-epr9281-ab154852'>ab154852</a>) at 1/5000 dilution

Lane 1:

MCF7 cell lysate at 20 µg

Lane 2:

T-47D cell lysate at 20 µg

Lane 3:

PC-3 cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

Predicted band size: 14 kDa

Observed band size: 14 kDa

false

• WB

Unknown

Western blot - Anti-PC4 antibody [EPR9281] - BSA and Azide free (AB249154)

This data was developed using ab154852, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PC4 antibody [EPR9281] (<a href='/en-us/products/primary-antibodies/pc4-antibody-epr9281-ab154852'>ab154852</a>) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

T47D cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

PC3 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 14 kDa

false