Anti-PCB antibody [10G7BF7]

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PCB antibody [10G7BF7] (AB123451)

ab123451 at 1µg/ml staining PCB in HDFn cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). DAPI was used to stain the cell nuclei (blue).

• WB

Supplier Data

Western blot - Anti-PCB antibody [10G7BF7] (AB123451)

ab123451 was shown to react with PC in wild-type HAP1 cells in Western blot with loss of signal observed in a PC knockout cell line. Wild-type HAP1 and PC knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab123451 overnight at 4 °C at a 1/500 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-PCB antibody [10G7BF7] (ab123451) at 1/500 dilution

Predicted band size: 130 kDa

false

• WB

Unknown

Western blot - Anti-PCB antibody [10G7BF7] (AB123451)

All lanes:

Western blot - Anti-PCB antibody [10G7BF7] (ab123451) at 1 µg/mL

Lane 1:

HepG2 cell lysate at 15 µg

Lane 2:

Hela at 15 µg

Lane 3:

H9C2 at 15 µg

Lane 4:

MEF at 15 µg

Lane 5:

Recombinant PC at 15 µg

Secondary

All lanes:

Goat-anti-Mouse HRP at 1/3000 dilution

Predicted band size: 130 kDa

false