Mouse Monoclonal PCB antibody. Suitable for ICC/IF, WB and reacts with Human, Rat, Mouse, Recombinant fragment samples.
IgG1
Mouse
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: 99% HEPES buffered saline
Liquid
Monoclonal
ICC/IF | WB | |
---|---|---|
Human | Tested | Tested |
Mouse | Expected | Expected |
Rat | Expected | Tested |
Recombinant fragment | Not recommended | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Recombinant fragment | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Select an associated product type
Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.
Pyruvic carboxylase, PCB
Mouse Monoclonal PCB antibody. Suitable for ICC/IF, WB and reacts with Human, Rat, Mouse, Recombinant fragment samples.
IgG1
Mouse
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: 99% HEPES buffered saline
Liquid
Monoclonal
10G7BF7
IgG fraction
kappa
Purity is near homogeneity as judged by SDS-PAGE. ab123451 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Blue Ice
+4°C
-20°C
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Product was previously marketed under the MitoSciences sub-brand.
This supplementary information is collated from multiple sources and compiled automatically.
The target 'PCB' also known as pyruvate carboxylase is a critical enzyme in metabolism. This enzyme has a significant molecular weight of around 130 kDa. It is expressed within the mitochondria of various tissues including liver kidney and adipose tissue. Pyruvate carboxylase catalyzes the carboxylation of pyruvate to form oxaloacetate which is an important step in several metabolic processes especially in gluconeogenesis.
Pyruvate carboxylase plays a significant role in maintaining metabolic balance. It is a biotin-dependent enzyme and part of a multienzyme complex that facilitates the conversion of pyruvate into oxaloacetate. This conversion is vital for replenishing intermediates in the citric acid cycle supporting the energy supply and anaplerotic reactions necessary for biosynthesis. The activity of pyruvate carboxylase also influences cellular levels of ATP and other nucleotides.
The carboxylation of pyruvate by pyruvate carboxylase integrates into gluconeogenesis and lipogenesis. In gluconeogenesis pyruvate carboxylase works alongside phosphoenolpyruvate carboxykinase (PEPCK) to achieve the synthesis of glucose from non-carbohydrate precursors. In lipogenesis oxaloacetate produced by pyruvate carboxylase is important for generating the precursor acetyl-CoA. These pathways involve pyruvate dehydrogenase and malate dehydrogenase reflecting the interconnectedness of metabolic functions.
Deficiencies or mutations in pyruvate carboxylase can lead to metabolic dysfunctions such as lactic acidosis and Leigh's disease. These conditions affect the body's ability to produce energy efficiently causing neurological and metabolic symptoms. Pyruvate carboxylase interacts closely with enzymes like lactate dehydrogenase and citrate synthase in these disorders highlighting its importance in metabolic homeostasis.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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All lanes: Western blot - Anti-PCB antibody [10G7BF7] (ab123451) at 1 µg/mL
Lane 1: HepG2 cell lysate at 15 µg
Lane 2: Hela at 15 µg
Lane 3: H9C2 at 15 µg
Lane 4: MEF at 15 µg
Lane 5: Recombinant PC at 15 µg
All lanes: Goat-anti-Mouse HRP at 1/3000 dilution
Predicted band size: 130 kDa
ab123451 at 1µg/ml staining PCB in HDFn cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). DAPI was used to stain the cell nuclei (blue).
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