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Mouse Monoclonal PCB antibody. Suitable for ICC/IF, WB and reacts with Human, Rat, Mouse, Recombinant fragment samples.

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Images

Western blot - Anti-PCB antibody [10G7BF7] (AB123451), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PCB antibody [10G7BF7] (AB123451), expandable thumbnail

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: 99% HEPES buffered saline

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWB
Human
Tested
Tested
Mouse
Expected
Expected
Rat
Expected
Tested
Recombinant fragment
Not recommended
Tested

Tested
Tested

Species

Human

Dilution info

1 µg/mL

Notes

-

Expected
Expected

Species

Rat

Dilution info

-

Notes

-

Species

Mouse

Dilution info

1 µg/mL

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment

Dilution info

-

Notes

-

Tested
Tested

Species

Rat

Dilution info

1 µg/mL

Notes

-

Species

Recombinant fragment

Dilution info

1 µg/mL

Notes

-

Species

Human

Dilution info

1 µg/mL

Notes

-

Expected
Expected

Species

Mouse

Dilution info

1 µg/mL

Notes

-

Associated Products

Select an associated product type

9 products for Alternative Product

Target data

Function

Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.

Alternative names

Recommended products

Mouse Monoclonal PCB antibody. Suitable for ICC/IF, WB and reacts with Human, Rat, Mouse, Recombinant fragment samples.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

10G7BF7

Purity

IgG fraction

Light chain type

kappa

Concentration
Loading...
Purification notes

Purity is near homogeneity as judged by SDS-PAGE. ab123451 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product was previously marketed under the MitoSciences sub-brand.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The target 'PCB' also known as pyruvate carboxylase is a critical enzyme in metabolism. This enzyme has a significant molecular weight of around 130 kDa. It is expressed within the mitochondria of various tissues including liver kidney and adipose tissue. Pyruvate carboxylase catalyzes the carboxylation of pyruvate to form oxaloacetate which is an important step in several metabolic processes especially in gluconeogenesis.

Biological function summary

Pyruvate carboxylase plays a significant role in maintaining metabolic balance. It is a biotin-dependent enzyme and part of a multienzyme complex that facilitates the conversion of pyruvate into oxaloacetate. This conversion is vital for replenishing intermediates in the citric acid cycle supporting the energy supply and anaplerotic reactions necessary for biosynthesis. The activity of pyruvate carboxylase also influences cellular levels of ATP and other nucleotides.

Pathways

The carboxylation of pyruvate by pyruvate carboxylase integrates into gluconeogenesis and lipogenesis. In gluconeogenesis pyruvate carboxylase works alongside phosphoenolpyruvate carboxykinase (PEPCK) to achieve the synthesis of glucose from non-carbohydrate precursors. In lipogenesis oxaloacetate produced by pyruvate carboxylase is important for generating the precursor acetyl-CoA. These pathways involve pyruvate dehydrogenase and malate dehydrogenase reflecting the interconnectedness of metabolic functions.

Associated diseases and disorders

Deficiencies or mutations in pyruvate carboxylase can lead to metabolic dysfunctions such as lactic acidosis and Leigh's disease. These conditions affect the body's ability to produce energy efficiently causing neurological and metabolic symptoms. Pyruvate carboxylase interacts closely with enzymes like lactate dehydrogenase and citrate synthase in these disorders highlighting its importance in metabolic homeostasis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-PCB antibody [10G7BF7] (ab123451), expandable thumbnail

    Western blot - Anti-PCB antibody [10G7BF7] (ab123451)

    All lanes: Western blot - Anti-PCB antibody [10G7BF7] (ab123451) at 1 µg/mL

    Lane 1: HepG2 cell lysate at 15 µg

    Lane 2: Hela at 15 µg

    Lane 3: H9C2 at 15 µg

    Lane 4: MEF at 15 µg

    Lane 5: Recombinant PC at 15 µg

    Secondary

    All lanes: Goat-anti-Mouse HRP at 1/3000 dilution

    Predicted band size: 130 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PCB antibody [10G7BF7] (ab123451), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PCB antibody [10G7BF7] (ab123451)

    ab123451 at 1µg/ml staining PCB in HDFn cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). DAPI was used to stain the cell nuclei (blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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