Mouse Monoclonal PCB antibody. Suitable for IP, Flow Cyt, ICC/IF and reacts with Human samples. Cited in 3 publications.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-PCB antibody [3H2AD9] (AB110314), expandable thumbnail](https://content.abcam.com/products/images/pcb-antibody-3h2ad9-ab110314--immunocytochemistry-immunofluorescence-img121202.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PCB antibody [3H2AD9] (AB110314)")
Publications
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- Molecular biomedicine 3:42022Long noncoding RNA RP11-241J12.3 targeting pyruvate carboxylase promotes hepatocellular carcinoma aggressiveness by disrupting pyruvate metabolism and the DNA mismatch repair system.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiuliu Cheng,Shichuan Hu,Jinhu Ma,Yongheng Shu,Yanwei Chen,Bin Zhang,Zhongbing Qi,Yunmeng Wang,Yan Zhang,Yuwei Zhang,Ping ChengPubMed 35122182
- Cell 168:210-223.e112017Nuclear Localization of Mitochondrial TCA Cycle Enzymes as a Critical Step in Mammalian Zygotic Genome Activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRaghavendra Nagaraj et. al.PubMed 28086092
- Nature communications 6:87842015Loss of succinate dehydrogenase activity results in dependency on pyruvate carboxylation for cellular anabolism.Applications:IFReactive species:Unspecified reactive speciesCharlotte Lussey-Lepoutre et. al.PubMed 26522426
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes For 2 hours. |
Associated Products
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Target data
Function
Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.
Alternative names
Pyruvic carboxylase, PCB, PC
Recommended products
Mouse Monoclonal PCB antibody. Suitable for IP, Flow Cyt, ICC/IF and reacts with Human samples. Cited in 3 publications.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: HEPES buffered saline- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- 3H2AD9
- Light chain type
- kappa
- Concentration
- Purification notes
ab110314 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.Purity: >95% by SDS-PAGE.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product was previously marketed under the MitoSciences sub-brand.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The target 'PCB' also known as pyruvate carboxylase is a critical enzyme in metabolism. This enzyme has a significant molecular weight of around 130 kDa. It is expressed within the mitochondria of various tissues including liver kidney and adipose tissue. Pyruvate carboxylase catalyzes the carboxylation of pyruvate to form oxaloacetate which is an important step in several metabolic processes especially in gluconeogenesis.
- Biological function summary
Pyruvate carboxylase plays a significant role in maintaining metabolic balance. It is a biotin-dependent enzyme and part of a multienzyme complex that facilitates the conversion of pyruvate into oxaloacetate. This conversion is vital for replenishing intermediates in the citric acid cycle supporting the energy supply and anaplerotic reactions necessary for biosynthesis. The activity of pyruvate carboxylase also influences cellular levels of ATP and other nucleotides.
- Pathways
The carboxylation of pyruvate by pyruvate carboxylase integrates into gluconeogenesis and lipogenesis. In gluconeogenesis pyruvate carboxylase works alongside phosphoenolpyruvate carboxykinase (PEPCK) to achieve the synthesis of glucose from non-carbohydrate precursors. In lipogenesis oxaloacetate produced by pyruvate carboxylase is important for generating the precursor acetyl-CoA. These pathways involve pyruvate dehydrogenase and malate dehydrogenase reflecting the interconnectedness of metabolic functions.
- Associated diseases and disorders
Deficiencies or mutations in pyruvate carboxylase can lead to metabolic dysfunctions such as lactic acidosis and Leigh's disease. These conditions affect the body's ability to produce energy efficiently causing neurological and metabolic symptoms. Pyruvate carboxylase interacts closely with enzymes like lactate dehydrogenase and citrate synthase in these disorders highlighting its importance in metabolic homeostasis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
![Immunocytochemistry/ Immunofluorescence - Anti-PCB antibody [3H2AD9] (ab110314), expandable thumbnail](https://content.abcam.com/products/images/pcb-antibody-3h2ad9-ab110314--immunocytochemistry-immunofluorescence-img121202.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PCB antibody [3H2AD9] (ab110314)")
Immunocytochemistry/ Immunofluorescence - Anti-PCB antibody [3H2AD9] (ab110314)
Upper image shows: Immunocytochemistry analysis using ab110314 at 5μg/ml staining PCB in Human fibroblast cells (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red).
Note: The target protein locates to the mitochondrial matrix.
Lower image shows cells co-stained with an antibody against PDH (green), an enzyme also located in the mitochondrial matrix. The composite image shows an identical mitochondrial pattern for both antibodies indicated by merged orange color.![Immunoprecipitation - Anti-PCB antibody [3H2AD9] (ab110314), expandable thumbnail](https://content.abcam.com/products/images/pcb-antibody-3h2ad9-ab110314--immunoprecipitation-img5656.gif "Immunoprecipitation - Anti-PCB antibody [3H2AD9] (ab110314)")
Immunoprecipitation - Anti-PCB antibody [3H2AD9] (ab110314)
Detection of ab110314 by immunoprecipitation staining of 130kDa PCB in human liver lysate.
All lanes: Immunoprecipitation - Anti-PCB antibody [3H2AD9] (ab110314)
Predicted band size: 130 kDa
![Flow Cytometry - Anti-PCB antibody [3H2AD9] (ab110314), expandable thumbnail](https://content.abcam.com/products/images/pcb-antibody-3h2ad9-ab110314--flow-cytometry-img82778.gif "Flow Cytometry - Anti-PCB antibody [3H2AD9] (ab110314)")
Flow Cytometry - Anti-PCB antibody [3H2AD9] (ab110314)
Flow cytometric analysis using ab110314 at 1µg/ml staining PCB in HeLa cells (blue). Isotype control antibody (red).
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