Anti-PCB antibody [3H2AD9]

• Flow Cyt

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Flow Cytometry - Anti-PCB antibody [3H2AD9] (AB110314)

Flow cytometric analysis using ab110314 at 1µg/ml staining PCB in HeLa cells (blue). Isotype control antibody (red).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-PCB antibody [3H2AD9] (AB110314)

Upper image shows : Immunocytochemistry analysis using ab110314 at 5μg/ml staining PCB in Human fibroblast cells (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red).
Note : The target protein locates to the mitochondrial matrix.
Lower image shows cells co-stained with an antibody against PDH (green), an enzyme also located in the mitochondrial matrix. The composite image shows an identical mitochondrial pattern for both antibodies indicated by merged orange color.

• IP

Supplier Data

Immunoprecipitation - Anti-PCB antibody [3H2AD9] (AB110314)

Immunoprecipitation of PC in HAP1 cells. Lysates were prepared and immunoprecipitation was performed using 2 μg of ab110314 pre-coupled to Protein A beads. Samples were then washed and processed for western blot.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Immunoprecipitation - Anti-PCB antibody [3H2AD9] (ab110314) at 2 µg

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HAP1 cell lysates

Predicted band size: 130 kDa

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• IP

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Immunoprecipitation - Anti-PCB antibody [3H2AD9] (AB110314)

Detection of ab110314 by immunoprecipitation staining of 130kDa PCB in human liver lysate.

All lanes:

Immunoprecipitation - Anti-PCB antibody [3H2AD9] (ab110314)

Predicted band size: 130 kDa

false