Goat Polyclonal PCB antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human PC aa 850-900.
Images
Publications
Filter by
- Molecular cell 82:3061-3076.e62022Lactate is an epigenetic metabolite that drives survival in model systems of glioblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesConsuelo Torrini,Trang Thi Thu Nguyen,Chang Shu,Angeliki Mela,Nelson Humala,Aayushi Mahajan,Erin Heather Seeley,Guoan Zhang,Mike-Andrew Westhoff,Georg Karpel-Massler,Jeffrey N Bruce,Peter Canoll,Markus D SiegelinPubMed 35948010
- Advanced science (Weinheim, Baden-Wurttemberg, Ger 6:19009722019Probiotics Ameliorate Colon Epithelial Injury Induced by Ambient Ultrafine Particles Exposure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaobo Li et. al.PubMed 31559135
- The Journal of physiology 595:6091-61202017Gene expression analyses reveal metabolic specifications in acute O -sensing chemoreceptor cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLin Gao,Victoria Bonilla-Henao,Paula García-Flores,Ignacio Arias-Mayenco,Patricia Ortega-Sáenz,José López-BarneoPubMed 28718507
- Nature communications 6:87842015Loss of succinate dehydrogenase activity results in dependency on pyruvate carboxylation for cellular anabolism.Applications:IHCReactive species:Unspecified reactive speciesCharlotte Lussey-Lepoutre et. al.PubMed 26522426
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PC aa 850-900. The exact immunogen used to generate this antibody is proprietary information. Database link P11498
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Expected | Tested |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Pig | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Dog, Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.03000-1.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Dog, Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Product
Target data
Function
Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.
Alternative names
Pyruvic carboxylase, PCB, PC
Recommended products
Goat Polyclonal PCB antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human PC aa 850-900.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PC aa 850-900. The exact immunogen used to generate this antibody is proprietary information. Database link P11498
- Purification technique
- Affinity purification Protein G
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The target 'PCB' also known as pyruvate carboxylase is a critical enzyme in metabolism. This enzyme has a significant molecular weight of around 130 kDa. It is expressed within the mitochondria of various tissues including liver kidney and adipose tissue. Pyruvate carboxylase catalyzes the carboxylation of pyruvate to form oxaloacetate which is an important step in several metabolic processes especially in gluconeogenesis.
- Biological function summary
Pyruvate carboxylase plays a significant role in maintaining metabolic balance. It is a biotin-dependent enzyme and part of a multienzyme complex that facilitates the conversion of pyruvate into oxaloacetate. This conversion is vital for replenishing intermediates in the citric acid cycle supporting the energy supply and anaplerotic reactions necessary for biosynthesis. The activity of pyruvate carboxylase also influences cellular levels of ATP and other nucleotides.
- Pathways
The carboxylation of pyruvate by pyruvate carboxylase integrates into gluconeogenesis and lipogenesis. In gluconeogenesis pyruvate carboxylase works alongside phosphoenolpyruvate carboxykinase (PEPCK) to achieve the synthesis of glucose from non-carbohydrate precursors. In lipogenesis oxaloacetate produced by pyruvate carboxylase is important for generating the precursor acetyl-CoA. These pathways involve pyruvate dehydrogenase and malate dehydrogenase reflecting the interconnectedness of metabolic functions.
- Associated diseases and disorders
Deficiencies or mutations in pyruvate carboxylase can lead to metabolic dysfunctions such as lactic acidosis and Leigh's disease. These conditions affect the body's ability to produce energy efficiently causing neurological and metabolic symptoms. Pyruvate carboxylase interacts closely with enzymes like lactate dehydrogenase and citrate synthase in these disorders highlighting its importance in metabolic homeostasis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCB antibody (ab115579)
ab115579, at 5µg/ml, staining PCB in Formalin-fixed, Paraffin-embedded Human Liver tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot - Anti-PCB antibody (ab115579)
All lanes: Western blot - Anti-PCB antibody (ab115579) at 0.03 µg/mL
All lanes: Mouse Liver lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 130 kDa
Observed band size: 150 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com