AB115579

Anti-PCB antibody

Name: Anti-PCB antibody (ab115579) | Abcam
Brand: Abcam Limited
SKU: AB115579
Price: 630 USD
Availability: InStock

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(5 Publications)

Goat Polyclonal PCB antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human PC aa 850-900.

View Alternative Names

Pyruvic carboxylase, PCB, PC

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCB antibody (AB115579)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCB antibody (AB115579)

ab115579, at 5µg/ml, staining PCB in Formalin-fixed, Paraffin-embedded Human Liver tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Unknown

Western blot - Anti-PCB antibody (AB115579)

All lanes:

Western blot - Anti-PCB antibody (ab115579) at 0.03 µg/mL

All lanes:

Mouse Liver lysate in RIPA buffer at 35 µg

Predicted band size: 130 kDa

Observed band size: 150 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human PC aa 850-900. The exact immunogen used to generate this antibody is proprietary information.

P11498

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.03-1 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target 'PCB' also known as pyruvate carboxylase is a critical enzyme in metabolism. This enzyme has a significant molecular weight of around 130 kDa. It is expressed within the mitochondria of various tissues including liver kidney and adipose tissue. Pyruvate carboxylase catalyzes the carboxylation of pyruvate to form oxaloacetate which is an important step in several metabolic processes especially in gluconeogenesis.

Biological function summary

Pyruvate carboxylase plays a significant role in maintaining metabolic balance. It is a biotin-dependent enzyme and part of a multienzyme complex that facilitates the conversion of pyruvate into oxaloacetate. This conversion is vital for replenishing intermediates in the citric acid cycle supporting the energy supply and anaplerotic reactions necessary for biosynthesis. The activity of pyruvate carboxylase also influences cellular levels of ATP and other nucleotides.

Pathways

The carboxylation of pyruvate by pyruvate carboxylase integrates into gluconeogenesis and lipogenesis. In gluconeogenesis pyruvate carboxylase works alongside phosphoenolpyruvate carboxykinase (PEPCK) to achieve the synthesis of glucose from non-carbohydrate precursors. In lipogenesis oxaloacetate produced by pyruvate carboxylase is important for generating the precursor acetyl-CoA. These pathways involve pyruvate dehydrogenase and malate dehydrogenase reflecting the interconnectedness of metabolic functions.

Deficiencies or mutations in pyruvate carboxylase can lead to metabolic dysfunctions such as lactic acidosis and Leigh's disease. These conditions affect the body's ability to produce energy efficiently causing neurological and metabolic symptoms. Pyruvate carboxylase interacts closely with enzymes like lactate dehydrogenase and citrate synthase in these disorders highlighting its importance in metabolic homeostasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.

See full target information PC

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

British journal of cancer 128:2116-2125 PubMed37016102

2023

Development of the semi-dry dot-blot method for intraoperative detecting micropapillary component in lung adenocarcinoma based on proteomics analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Long Xu,Hang Su,Shengnan Zhao,Haojie Si,Huikang Xie,Yijiu Ren,Jiani Gao,Fang Wang,Xiaofeng Xie,Chenyang Dai,Chunyan Wu,Deping Zhao,Chang Chen

Molecular cell 82:3061-3076.e6 PubMed35948010

2022

Lactate is an epigenetic metabolite that drives survival in model systems of glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Consuelo Torrini,Trang Thi Thu Nguyen,Chang Shu,Angeliki Mela,Nelson Humala,Aayushi Mahajan,Erin Heather Seeley,Guoan Zhang,Mike-Andrew Westhoff,Georg Karpel-Massler,Jeffrey N Bruce,Peter Canoll,Markus D Siegelin

Advanced science (Weinheim, Baden-Wurttemberg, Ger 6:1900972 PubMed31559135

2019

Probiotics Ameliorate Colon Epithelial Injury Induced by Ambient Ultrafine Particles Exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaobo Li,Hao Sun,Bin Li,Xinwei Zhang,Jian Cui,Jun Yun,Yiping Yang,Li'e Zhang,Qingtao Meng,Shenshen Wu,Junchao Duan,Hongbao Yang,Jiong Wu,Zhiwei Sun,Yunfeng Zou,Rui Chen

The Journal of physiology 595:6091-6120 PubMed28718507

2017

Gene expression analyses reveal metabolic specifications in acute O -sensing chemoreceptor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Gao,Victoria Bonilla-Henao,Paula García-Flores,Ignacio Arias-Mayenco,Patricia Ortega-Sáenz,José López-Barneo

Nature communications 6:8784 PubMed26522426

2015

Loss of succinate dehydrogenase activity results in dependency on pyruvate carboxylation for cellular anabolism.

Applications

IHC

Species

Unspecified reactive species

Charlotte Lussey-Lepoutre,Kate E R Hollinshead,Christian Ludwig,Mélanie Menara,Aurélie Morin,Luis-Jaime Castro-Vega,Seth J Parker,Maxime Janin,Cosimo Martinelli,Chris Ottolenghi,Christian Metallo,Anne-Paule Gimenez-Roqueplo,Judith Favier,Daniel A Tennant

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PCB antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCB antibody (AB115579)

Western blot - Anti-PCB antibody (AB115579)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise