AB229267

Anti-PCB antibody - C-terminal

Name: Anti-PCB antibody - C-terminal (ab229267) | Abcam
Brand: Abcam Limited
SKU: AB229267
Price: 525 USD
Availability: InStock

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(7 Publications)

Rabbit Polyclonal PCB antibody. C-terminal. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Rat samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PC.

View Alternative Names

Pyruvic carboxylase, PCB, PC

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-PCB antibody - C-terminal (AB229267)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PCB antibody - C-terminal (AB229267)

2% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PCB (green) using ab229267 at 1/500 dilution in ICC/IF.

Blue : Hoechst 33342 staining.

Supplier Data

Immunoprecipitation - Anti-PCB antibody - C-terminal (AB229267)

PCB was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5μg ab229267. Western blot was performed from the immunoprecipitate using ab229267. Anti-rabbit IgG was used as secondary antibody.

Lane 1 : HeLa whole cell extract.

Lane 2 : Control IgG instead of ab229267 in HeLa whole cell extract.

Lane 3 : ab229267 IP in HeLa whole cell extract.

All lanes:

Immunoprecipitation - Anti-PCB antibody - C-terminal (ab229267)

Predicted band size: 130 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCB antibody - C-terminal (AB229267)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCB antibody - C-terminal (AB229267)

Paraffin-embedded rat duodenum tissue stained for PCB using ab229267 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-PCB antibody - C-terminal (AB229267)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-PCB antibody - C-terminal (ab229267) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg

Predicted band size: 130 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

IP, ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PC. The exact immunogen used to generate this antibody is proprietary information.

P11498

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/857", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target 'PCB' also known as pyruvate carboxylase is a critical enzyme in metabolism. This enzyme has a significant molecular weight of around 130 kDa. It is expressed within the mitochondria of various tissues including liver kidney and adipose tissue. Pyruvate carboxylase catalyzes the carboxylation of pyruvate to form oxaloacetate which is an important step in several metabolic processes especially in gluconeogenesis.

Biological function summary

Pyruvate carboxylase plays a significant role in maintaining metabolic balance. It is a biotin-dependent enzyme and part of a multienzyme complex that facilitates the conversion of pyruvate into oxaloacetate. This conversion is vital for replenishing intermediates in the citric acid cycle supporting the energy supply and anaplerotic reactions necessary for biosynthesis. The activity of pyruvate carboxylase also influences cellular levels of ATP and other nucleotides.

Pathways

The carboxylation of pyruvate by pyruvate carboxylase integrates into gluconeogenesis and lipogenesis. In gluconeogenesis pyruvate carboxylase works alongside phosphoenolpyruvate carboxykinase (PEPCK) to achieve the synthesis of glucose from non-carbohydrate precursors. In lipogenesis oxaloacetate produced by pyruvate carboxylase is important for generating the precursor acetyl-CoA. These pathways involve pyruvate dehydrogenase and malate dehydrogenase reflecting the interconnectedness of metabolic functions.

Deficiencies or mutations in pyruvate carboxylase can lead to metabolic dysfunctions such as lactic acidosis and Leigh's disease. These conditions affect the body's ability to produce energy efficiently causing neurological and metabolic symptoms. Pyruvate carboxylase interacts closely with enzymes like lactate dehydrogenase and citrate synthase in these disorders highlighting its importance in metabolic homeostasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.

See full target information PC

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:7414 PubMed36460681

2022

Nuclear localization of mitochondrial TCA cycle enzymes modulates pluripotency via histone acetylation.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Li,Qi Long,Hao Wu,Yanshuang Zhou,Lifan Duan,Hao Yuan,Yingzhe Ding,Yile Huang,Yi Wu,Jinyu Huang,Delong Liu,Baodan Chen,Jian Zhang,Juntao Qi,Shiwei Du,Linpeng Li,Yang Liu,Zifeng Ruan,Zihuang Liu,Zichao Liu,Yifan Zhao,Jianghuan Lu,Junwei Wang,Wai-Yee Chan,Xingguo Liu

American journal of cancer research 12:3729-3744 PubMed36119843

2022

NDRG2 inhibits pyruvate carboxylase-mediated anaplerosis and combines with glutamine blockade to inhibit the proliferation of glioma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jiancai Wang,Xiang Sun,Jiayuan Wang,Kun Zhang,Yiyi Yuan,Yan Guo,Libo Yao,Xia Li,Lan Shen

Evidence-based complementary and alternative medicine : eCAM 2021:9922292 PubMed34422083

2021

Effect of Gegen Qinlian Decoction on Hepatic Gluconeogenesis in ZDF Rats with Type 2 Diabetes Mellitus Based on the Farnesol Receptor/Ceramide Signaling Pathway Regulating Mitochondrial Metabolism and Endoplasmic Reticulum Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Zhou,Ning Song,Shi-Qi Wang,Yan Wang,Yi-Kun Zhao,Xiang-Dong Zhu

Arteriosclerosis, thrombosis, and vascular biology 41:734-754 PubMed33297749

2020

Single Mutation in the Gene Metabolically Reprograms Pulmonary Artery Smooth Muscle Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Joel James,Marina Zemskova,Cody A Eccles,Mathews V Varghese,Maki Niihori,Natalie K Barker,Moulun Luo,Lawrence J Mandarino,Paul R Langlais,Olga Rafikova,Ruslan Rafikov

Scientific reports 10:14589 PubMed32883997

2020

Lipocalin-2 deficiency may predispose to the progression of spontaneous age-related adiposity in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Keya Meyers,María López,Joanna Ho,Savannah Wills,Srujana Rayalam,Shashidharamurthy Taval

Journal of clinical medicine 9: PubMed32041182

2020

Inhibition of Anaplerosis Attenuated Vascular Proliferation in Pulmonary Arterial Hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Mathews Valuparampil Varghese,Joel James,Cody A Eccles,Maki Niihori,Olga Rafikova,Ruslan Rafikov

Antioxidants (Basel, Switzerland) 9: PubMed31991719

2020

Antioxidant-Conjugated Peptide Attenuated Metabolic Reprogramming in Pulmonary Hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Mathews Valuparampil Varghese,Maki Niihori,Cody A Eccles,Sergey Kurdyukov,Joel James,Olga Rafikova,Ruslan Rafikov

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-PCB antibody - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-PCB antibody - C-terminal (AB229267)

Immunoprecipitation - Anti-PCB antibody - C-terminal (AB229267)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCB antibody - C-terminal (AB229267)

Western blot - Anti-PCB antibody - C-terminal (AB229267)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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