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AB229267

Anti-PCB antibody - C-terminal

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(7 Publications )

Rabbit Polyclonal PCB antibody. C-terminal. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Rat samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PC.
2 Images
Immunocytochemistry/ Immunofluorescence - Anti-PCB antibody - C-terminal (AB229267)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PCB antibody - C-terminal (AB229267)

2% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PCB (green) using ab229267 at 1/500 dilution in ICC/IF.

Blue : Hoechst 33342 staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCB antibody - C-terminal (AB229267)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCB antibody - C-terminal (AB229267)

Paraffin-embedded rat duodenum tissue stained for PCB using ab229267 at 1/500 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

IHC-P, WB, ICC/IF, IP

applications

Immunogen

Recombinant Fragment Protein within Human PC. The exact immunogen used to generate this antibody is proprietary information.

P11498

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/857", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target 'PCB' also known as pyruvate carboxylase is a critical enzyme in metabolism. This enzyme has a significant molecular weight of around 130 kDa. It is expressed within the mitochondria of various tissues including liver kidney and adipose tissue. Pyruvate carboxylase catalyzes the carboxylation of pyruvate to form oxaloacetate which is an important step in several metabolic processes especially in gluconeogenesis.
Biological function summary

Pyruvate carboxylase plays a significant role in maintaining metabolic balance. It is a biotin-dependent enzyme and part of a multienzyme complex that facilitates the conversion of pyruvate into oxaloacetate. This conversion is vital for replenishing intermediates in the citric acid cycle supporting the energy supply and anaplerotic reactions necessary for biosynthesis. The activity of pyruvate carboxylase also influences cellular levels of ATP and other nucleotides.

Pathways

The carboxylation of pyruvate by pyruvate carboxylase integrates into gluconeogenesis and lipogenesis. In gluconeogenesis pyruvate carboxylase works alongside phosphoenolpyruvate carboxykinase (PEPCK) to achieve the synthesis of glucose from non-carbohydrate precursors. In lipogenesis oxaloacetate produced by pyruvate carboxylase is important for generating the precursor acetyl-CoA. These pathways involve pyruvate dehydrogenase and malate dehydrogenase reflecting the interconnectedness of metabolic functions.

Deficiencies or mutations in pyruvate carboxylase can lead to metabolic dysfunctions such as lactic acidosis and Leigh's disease. These conditions affect the body's ability to produce energy efficiently causing neurological and metabolic symptoms. Pyruvate carboxylase interacts closely with enzymes like lactate dehydrogenase and citrate synthase in these disorders highlighting its importance in metabolic homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.
See full target information PC

Alternative Names

Pyruvic carboxylase, PCB, PC
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Publications (7)

Recent publications for all applications. Explore the full listand refine your search

Nature communications 13:7414 PubMed36460681

2022

Nuclear localization of mitochondrial TCA cycle enzymes modulates pluripotency via histone acetylation.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Li,Qi Long,Hao Wu,Yanshuang Zhou,Lifan Duan,Hao Yuan,Yingzhe Ding,Yile Huang,Yi Wu,Jinyu Huang,Delong Liu,Baodan Chen,Jian Zhang,Juntao Qi,Shiwei Du,Linpeng Li,Yang Liu,Zifeng Ruan,Zihuang Liu,Zichao Liu,Yifan Zhao,Jianghuan Lu,Junwei Wang,Wai-Yee Chan,Xingguo Liu

American journal of cancer research 12:3729-3744 PubMed36119843

2022

NDRG2 inhibits pyruvate carboxylase-mediated anaplerosis and combines with glutamine blockade to inhibit the proliferation of glioma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jiancai Wang,Xiang Sun,Jiayuan Wang,Kun Zhang,Yiyi Yuan,Yan Guo,Libo Yao,Xia Li,Lan Shen

Evidence-based complementary and alternative medicine : eCAM 2021:9922292 PubMed34422083

2021

Effect of Gegen Qinlian Decoction on Hepatic Gluconeogenesis in ZDF Rats with Type 2 Diabetes Mellitus Based on the Farnesol Receptor/Ceramide Signaling Pathway Regulating Mitochondrial Metabolism and Endoplasmic Reticulum Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Zhou,Ning Song,Shi-Qi Wang,Yan Wang,Yi-Kun Zhao,Xiang-Dong Zhu

Arteriosclerosis, thrombosis, and vascular biology 41:734-754 PubMed33297749

2020

Single Mutation in the Gene Metabolically Reprograms Pulmonary Artery Smooth Muscle Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Joel James,Marina Zemskova,Cody A Eccles,Mathews V Varghese,Maki Niihori,Natalie K Barker,Moulun Luo,Lawrence J Mandarino,Paul R Langlais,Olga Rafikova,Ruslan Rafikov

Scientific reports 10:14589 PubMed32883997

2020

Lipocalin-2 deficiency may predispose to the progression of spontaneous age-related adiposity in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Keya Meyers,María López,Joanna Ho,Savannah Wills,Srujana Rayalam,Shashidharamurthy Taval

Journal of clinical medicine 9: PubMed32041182

2020

Inhibition of Anaplerosis Attenuated Vascular Proliferation in Pulmonary Arterial Hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Mathews Valuparampil Varghese,Joel James,Cody A Eccles,Maki Niihori,Olga Rafikova,Ruslan Rafikov

Antioxidants (Basel, Switzerland) 9: PubMed31991719

2020

Antioxidant-Conjugated Peptide Attenuated Metabolic Reprogramming in Pulmonary Hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Mathews Valuparampil Varghese,Maki Niihori,Cody A Eccles,Sergey Kurdyukov,Joel James,Olga Rafikova,Ruslan Rafikov
View all publications
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Product promise

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