AB109577

Anti-PCBP1 antibody

Name: Anti-PCBP1 antibody (ab109577) | Abcam
Brand: Abcam Limited
SKU: AB109577
Price: 535 USD
Availability: InStock

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(4 Publications)

Goat Polyclonal PCBP1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human PCBP1 aa 200-250.

View Alternative Names

Poly(rC)-binding protein 1, Alpha-CP1, Heterogeneous nuclear ribonucleoprotein E1, Nucleic acid-binding protein SUB2.3, hnRNP E1, PCBP1

1 Images

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Western blot - Anti-PCBP1 antibody (AB109577)

All lanes:

Western blot - Anti-PCBP1 antibody (ab109577) at 0.01 µg/mL

All lanes:

HeLa lysate (in RIPA buffer) at 35 µg

Predicted band size: 37 kDa

Observed band size: 38 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human PCBP1 aa 200-250. The exact immunogen used to generate this antibody is proprietary information.

Q15365

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01-0.03 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PCBP1 protein also known as Poly(rC) Binding Protein 1 or hnRNP E1 is a RNA-binding protein with a mass of approximately 39-40 kDa. Mechanically it binds to single-stranded poly(C) regions in RNA through its three K-homology (KH) domains. These domains allow PCBP1 to regulate mRNA stability translation and splicing. The protein is broadly expressed across many tissues with high levels in the liver thymus kidneys and the brain suggesting its essential involvement in diverse cellular processes.

Biological function summary

PCBP1 influences the cellular machinery through its role in post-transcriptional regulation. It does not function alone but rather exists as part of an RNA-protein complex. This protein participates in modulating mRNA turnover and translational silencing playing important roles in cellular iron homeostasis and response to viral infections. Through its interaction with specific mRNAs PCBP1 helps regulate processes like cell proliferation and differentiation where its dysregulation may have significant consequences.

Pathways

Alterations in PCBP1 activities impact important cellular pathways such as the Wnt signaling pathway and the iron metabolism pathway. In the Wnt signaling pathway PCBP1 interacts with other proteins like beta-catenin to regulate gene expression that guides cell growth and division. In iron metabolism PCBP1 plays a role by stabilizing iron-responsive element (IRE)-containing mRNAs therefore influencing the expression of proteins involved in iron storage and transport.

PCBP1 has connections to cancer and anemia. In various cancers such as colorectal and lung cancer PCBP1 expression levels change suggesting a role in tumor progression and metastasis. It interacts with proteins such as beta-catenin which is known to contribute to oncogenic pathways. In conditions of anemia particularly sideroblastic anemia PCBP1 influences the expression of proteins that are critical for iron regulation like ferritin affecting iron homeostasis in red blood cells. Understanding these relationships provides potential avenues for therapeutic interventions targeting PCBP1 and associated pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Single-stranded nucleic acid binding protein that binds preferentially to oligo dC (PubMed : 15731341, PubMed : 7556077, PubMed : 7607214, PubMed : 8152927). Together with PCBP2, required for erythropoiesis, possibly by regulating mRNA splicing (By similarity).. (Microbial infection) In case of infection by poliovirus, plays a role in initiation of viral RNA replication in concert with the viral protein 3CD.

See full target information PCBP1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature structural & molecular biology 31:1341-1354 PubMed38658621

2024

Alternative splicing of CARM1 regulated by LincGET-guided paraspeckles biases the first cell fate in mammalian early embryos.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaqiang Wang,Yiwei Zhang,Jiaze Gao,Guihai Feng,Chao Liu,Xueke Li,Pengcheng Li,Zhonghua Liu,Falong Lu,Leyun Wang,Wei Li,Qi Zhou,Yusheng Liu

Molecular carcinogenesis 62:907-919 PubMed37157950

2023

PCBP1 protects bladder cancer cells from mitochondria injury and ferroptosis by inducing LACTB mRNA degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Luo,Yunli Zhang,Shiyu Pang,Jingxian Min,Tao Wang,Dali Wu,Chun Lin,Zebin Xiao,Qi Xiang,Qing Li,Lili Ma

Journal of translational medicine 20:343 PubMed35907982

2022

The RNA-binding protein PCBP1 represses lung adenocarcinoma progression by stabilizing DKK1 mRNA and subsequently downregulating β-catenin.

Applications

Unspecified application

Species

Unspecified reactive species

Yujia Zheng,Zheng Zhou,Ran Wei,Chu Xiao,Hao Zhang,Tao Fan,Bo Zheng,Chunxiang Li,Jie He

Acta virologica 62:287-293 PubMed30160144

2018

Induction and antiviral activity of human β-defensin 3 in intestinal cells with picornavirus infection.

Applications

Unspecified application

Species

Unspecified reactive species

W Chen,Z Liu,Q Zhang,Q Yan,S Jing

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-PCBP1 antibody

Western blot - Anti-PCBP1 antibody (AB109577)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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