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AB168377

Anti-PCBP1 antibody [EPR11049(B)]

1

(1 Review)

|

(6 Publications)

Rabbit Recombinant Monoclonal PCBP1 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

View Alternative Names

Poly(rC)-binding protein 1, Alpha-CP1, Heterogeneous nuclear ribonucleoprotein E1, Nucleic acid-binding protein SUB2.3, hnRNP E1, PCBP1

2 Images
Immunoprecipitation - Anti-PCBP1 antibody [EPR11049(B)] (AB168377)
  • IP

Unknown

Immunoprecipitation - Anti-PCBP1 antibody [EPR11049(B)] (AB168377)

Western blot analysis on HeLa cell lysate pellet immunoprecipitated using ab168377 at a 1/10 dilution, and an HRP-conjugated anti-rabbit IgG.

All lanes:

Immunoprecipitation - Anti-PCBP1 antibody [EPR11049(B)] (ab168377)

Predicted band size: 37 kDa

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Western blot - Anti-PCBP1 antibody [EPR11049(B)] (AB168377)
  • WB

Unknown

Western blot - Anti-PCBP1 antibody [EPR11049(B)] (AB168377)

All lanes:

Western blot - Anti-PCBP1 antibody [EPR11049(B)] (ab168377) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Predicted band size: 37 kDa

false

  • Carrier free

    Anti-PCBP1 antibody [EPR11049(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11049(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PCBP1 protein also known as Poly(rC) Binding Protein 1 or hnRNP E1 is a RNA-binding protein with a mass of approximately 39-40 kDa. Mechanically it binds to single-stranded poly(C) regions in RNA through its three K-homology (KH) domains. These domains allow PCBP1 to regulate mRNA stability translation and splicing. The protein is broadly expressed across many tissues with high levels in the liver thymus kidneys and the brain suggesting its essential involvement in diverse cellular processes.
Biological function summary

PCBP1 influences the cellular machinery through its role in post-transcriptional regulation. It does not function alone but rather exists as part of an RNA-protein complex. This protein participates in modulating mRNA turnover and translational silencing playing important roles in cellular iron homeostasis and response to viral infections. Through its interaction with specific mRNAs PCBP1 helps regulate processes like cell proliferation and differentiation where its dysregulation may have significant consequences.

Pathways

Alterations in PCBP1 activities impact important cellular pathways such as the Wnt signaling pathway and the iron metabolism pathway. In the Wnt signaling pathway PCBP1 interacts with other proteins like beta-catenin to regulate gene expression that guides cell growth and division. In iron metabolism PCBP1 plays a role by stabilizing iron-responsive element (IRE)-containing mRNAs therefore influencing the expression of proteins involved in iron storage and transport.

PCBP1 has connections to cancer and anemia. In various cancers such as colorectal and lung cancer PCBP1 expression levels change suggesting a role in tumor progression and metastasis. It interacts with proteins such as beta-catenin which is known to contribute to oncogenic pathways. In conditions of anemia particularly sideroblastic anemia PCBP1 influences the expression of proteins that are critical for iron regulation like ferritin affecting iron homeostasis in red blood cells. Understanding these relationships provides potential avenues for therapeutic interventions targeting PCBP1 and associated pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Single-stranded nucleic acid binding protein that binds preferentially to oligo dC (PubMed : 15731341, PubMed : 7556077, PubMed : 7607214, PubMed : 8152927). Together with PCBP2, required for erythropoiesis, possibly by regulating mRNA splicing (By similarity).. (Microbial infection) In case of infection by poliovirus, plays a role in initiation of viral RNA replication in concert with the viral protein 3CD.
See full target information PCBP1
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40362430

2025

Dysregulation of Labile Iron Predisposes Chemotherapy Resistant Cancer Cells to Ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Luke V Loftus,Louis T A Rolle,Bowen Wang,Kenneth J Pienta,Sarah R Amend

Redox biology 62:102708 PubMed37116254

2023

17β-oestradiol inhibits ferroptosis in the hippocampus by upregulating DHODH and further improves memory decline after ovariectomy.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Tian,Yuan Xie,Zaixin Guo,Penghui Feng,Yang You,Qi Yu

Cell death & disease 12:42 PubMed33414446

2021

TRIB2 modulates proteasome function to reduce ubiquitin stability and protect liver cancer cells against oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Susu Guo,Yuxin Chen,Yueyue Yang,Xiao Zhang,Lifang Ma,Xiangfei Xue,Yongxia Qiao,Jiayi Wang

Frontiers in pharmacology 11:703 PubMed32536864

2020

SNHG1 Inhibits ox-LDL-Induced Inflammatory Response and Apoptosis of HUVECs Up-Regulating GNAI2 and PCBP1.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Lu,Jue Xi,Yao Zhang,Wensu Chen,Fengyun Zhang,Chenzong Li,Zhirong Wang

Molecular therapy. Nucleic acids 19:1368-1378 PubMed32160707

2020

The Identification of Long Non-coding RNA H19 Target and Its Function in Chronic Myeloid Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Juhua Yang,Zhao Yin,Yumin Li,Yanjun Liu,Guiping Huang,Chunming Gu,Jia Fei

Haematologica 101:437-47 PubMed26802051

2016

Extracellular vesicle miR-7977 is involved in hematopoietic dysfunction of mesenchymal stromal cells via poly(rC) binding protein 1 reduction in myeloid neoplasms.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroto Horiguchi,Masayoshi Kobune,Shohei Kikuchi,Masahiro Yoshida,Masaki Murata,Kazuyuki Murase,Satoshi Iyama,Kohichi Takada,Tsutomu Sato,Kaoru Ono,Akari Hashimoto,Ayumi Tatekoshi,Yusuke Kamihara,Yutaka Kawano,Koji Miyanishi,Norimasa Sawada,Junji Kato
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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