AB168378

Anti-PCBP1 antibody [EPR11055]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(8 Publications)

Rabbit Recombinant Monoclonal PCBP1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 8 publications.

View Alternative Names

Poly(rC)-binding protein 1, Alpha-CP1, Heterogeneous nuclear ribonucleoprotein E1, Nucleic acid-binding protein SUB2.3, hnRNP E1, PCBP1

2 Images

Lab

Immunoprecipitation - Anti-PCBP1 antibody [EPR11055] (AB168378)

Purified ab168378 at 1 : 50 dilution (2µg) immunoprecipitating PCBP1 in 293T whole cell lysate.
Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-PCBP1 antibody [EPR11055] (ab168378) at 1/50 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) whole cell lysate at 10 µg

Lane 2:

ab168378 + 293T whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab168378 in 293T whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 37 kDa

false

Unknown

Western blot - Anti-PCBP1 antibody [EPR11055] (AB168378)

All lanes:

Western blot - Anti-PCBP1 antibody [EPR11055] (ab168378) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate

Lane 2:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate

Lane 3:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 37 kDa

false

Carrier free

Anti-PCBP1 antibody [EPR11055] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11055

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PCBP1 protein also known as Poly(rC) Binding Protein 1 or hnRNP E1 is a RNA-binding protein with a mass of approximately 39-40 kDa. Mechanically it binds to single-stranded poly(C) regions in RNA through its three K-homology (KH) domains. These domains allow PCBP1 to regulate mRNA stability translation and splicing. The protein is broadly expressed across many tissues with high levels in the liver thymus kidneys and the brain suggesting its essential involvement in diverse cellular processes.

Biological function summary

PCBP1 influences the cellular machinery through its role in post-transcriptional regulation. It does not function alone but rather exists as part of an RNA-protein complex. This protein participates in modulating mRNA turnover and translational silencing playing important roles in cellular iron homeostasis and response to viral infections. Through its interaction with specific mRNAs PCBP1 helps regulate processes like cell proliferation and differentiation where its dysregulation may have significant consequences.

Pathways

Alterations in PCBP1 activities impact important cellular pathways such as the Wnt signaling pathway and the iron metabolism pathway. In the Wnt signaling pathway PCBP1 interacts with other proteins like beta-catenin to regulate gene expression that guides cell growth and division. In iron metabolism PCBP1 plays a role by stabilizing iron-responsive element (IRE)-containing mRNAs therefore influencing the expression of proteins involved in iron storage and transport.

PCBP1 has connections to cancer and anemia. In various cancers such as colorectal and lung cancer PCBP1 expression levels change suggesting a role in tumor progression and metastasis. It interacts with proteins such as beta-catenin which is known to contribute to oncogenic pathways. In conditions of anemia particularly sideroblastic anemia PCBP1 influences the expression of proteins that are critical for iron regulation like ferritin affecting iron homeostasis in red blood cells. Understanding these relationships provides potential avenues for therapeutic interventions targeting PCBP1 and associated pathways.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Single-stranded nucleic acid binding protein that binds preferentially to oligo dC (PubMed : 15731341, PubMed : 7556077, PubMed : 7607214, PubMed : 8152927). Together with PCBP2, required for erythropoiesis, possibly by regulating mRNA splicing (By similarity).. (Microbial infection) In case of infection by poliovirus, plays a role in initiation of viral RNA replication in concert with the viral protein 3CD.

See full target information PCBP1

style

left-column

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40362430

2025

Dysregulation of Labile Iron Predisposes Chemotherapy Resistant Cancer Cells to Ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Luke V Loftus,Louis T A Rolle,Bowen Wang,Kenneth J Pienta,Sarah R Amend

American journal of physiology. Lung cellular and molecular physiology 328:L571-L585 PubMed40095970

2025

Therapeutic strategies to reverse cigarette smoke-induced ion channel and mucociliary dysfunction in COPD airway epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Neerupma Silswal,Nathalie Baumlin,Steven Haworth,Robert N Montgomery,Makoto Yoshida,John S Dennis,Sireesha Yerrathota,Michael D Kim,Matthias Salathe

Viruses 14: PubMed35215884

2022

Poly(rC)-Binding Protein 1 Limits Hepatitis C Virus Virion Assembly and Secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Sophie E Cousineau,Marylin Rheault,Selena M Sagan

Nature 588:157-163 PubMed33239784

2020

Splicing factor YBX1 mediates persistence of JAK2-mutated neoplasms.

Applications

Unspecified application

Species

Unspecified reactive species

Ashok Kumar Jayavelu,Tina M Schnöder,Florian Perner,Carolin Herzog,Arno Meiler,Gurumoorthy Krishnamoorthy,Nicolas Huber,Juliane Mohr,Bärbel Edelmann-Stephan,Rebecca Austin,Sabine Brandt,Francesca Palandri,Nicolas Schröder,Berend Isermann,Frank Edlich,Amit U Sinha,Martin Ungelenk,Christian A Hübner,Robert Zeiser,Susann Rahmig,Claudia Waskow,Iain Coldham,Thomas Ernst,Andreas Hochhaus,Stefanie Jilg,Philipp J Jost,Ann Mullally,Lars Bullinger,Peter R Mertens,Steven W Lane,Matthias Mann,Florian H Heidel

Biochimica et biophysica acta. Molecular basis of 1866:165844 PubMed32480040

2020

Acireductone dioxygenase 1 (ADI1) is regulated by cellular iron by a mechanism involving the iron chaperone, PCBP1, with PCBP2 acting as a potential co-chaperone.

Applications

Unspecified application

Species

Unspecified reactive species

Dong-Hun Bae,Darius J R Lane,Aritee R Siafakas,Robert Sutak,Jasmina Paluncic,Michael L H Huang,Patric J Jansson,Yohan Suryo Rahmanto,Des R Richardson

Cellular and molecular life sciences : CMLS 78:949-962 PubMed32440711

2020

TGF-β1 promotes epithelial-to-mesenchymal transition and stemness of prostate cancer cells by inducing PCBP1 degradation and alternative splicing of CD44.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Chen,Meng Gu,Zhi-Kang Cai,Hu Zhao,Shi-Cheng Sun,Chong Liu,Ming Zhan,Yan-Bo Chen,Zhong Wang

Cell reports 30:4355-4369.e6 PubMed32234473

2020

Nutlin-Induced Apoptosis Is Specified by a Translation Program Regulated by PCBP2 and DHX30.

Applications

Unspecified application

Species

Unspecified reactive species

Dario Rizzotto,Sara Zaccara,Annalisa Rossi,Matthew D Galbraith,Zdenek Andrysik,Ahwan Pandey,Kelly D Sullivan,Alessandro Quattrone,Joaquín M Espinosa,Erik Dassi,Alberto Inga

Molecular and cellular biology 36:304-19 PubMed26527618

2015

The Poly(C) Binding Protein Pcbp2 and Its Retrotransposed Derivative Pcbp1 Are Independently Essential to Mouse Development.

Applications

Unspecified application

Species

Unspecified reactive species

Louis R Ghanem,Andrew Kromer,Ian M Silverman,Priya Chatterji,Elizabeth Traxler,Alfredo Penzo-Mendez,Mitchell J Weiss,Ben Z Stanger,Stephen A Liebhaber

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PCBP1 antibody [EPR11055]

Immunoprecipitation - Anti-PCBP1 antibody [EPR11055] (AB168378)

Western blot - Anti-PCBP1 antibody [EPR11055] (AB168378)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise