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AB95942

Anti-PCBP2/hnRNP E2 antibody

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(3 Publications)

Rabbit Polyclonal PCBP2/hnRNP E2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Poly(rC)-binding protein 2, Alpha-CP2, Heterogeneous nuclear ribonucleoprotein E2, hnRNP E2, PCBP2

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-PCBP2/hnRNP E2 antibody (AB95942)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PCBP2/hnRNP E2 antibody (AB95942)

ab95942, at 1/200 dilution, staining PCBP2/hnRNP E2 in paraformaldehyde-fixed HeLa cells by Immunofluorescence. Right image is merged with DNA probe.

Western blot - Anti-PCBP2/hnRNP E2 antibody (AB95942)
  • WB

Unknown

Western blot - Anti-PCBP2/hnRNP E2 antibody (AB95942)

10% SDS PAGE

All lanes:

Western blot - Anti-PCBP2/hnRNP E2 antibody (ab95942) at 1/500 dilution

All lanes:

HepG2 whole cell lysate at 30 µg

Predicted band size: 39 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

AB200835

Anti-PCBP2/hnRNP E2 antibody [EPR14859(2)]

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Primary Antibodies

AB236137

Anti-PCBP2/hnRNP E2 antibody [EPR14858] - BSA and Azide free

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCBP2/hnRNP E2 antibody [EPR14858] - BSA and Azide free (AB236137)

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  • 0
Primary Antibodies

AB184962

Anti-PCBP2/hnRNP E2 antibody [EPR14858]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCBP2/hnRNP E2 antibody [EPR14858] (AB184962)

  • 0
  • 0

Complementary Products

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Secondary Antibodies

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Assay Kits

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Primary Antibodies

AB97959

Anti-SOX2 antibody

BOND RX™ Validated

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  • 4
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCBP2 also known as hnRNP E2 is a poly(C) binding protein with a molecular weight of approximately 38 kDa. It binds to single-stranded poly(C) sequences in RNA molecules. PCBP2 is widely expressed in various tissues where it modulates pre-mRNA splicing RNA stability and translation. It functions as part of ribonucleoprotein complexes and is known for its role in regulating mRNA transport and localization within the cell.
Biological function summary

A significant function of PCBP2 involves the maintenance of mRNA stability particularly for certain growth factor and cytokine mRNAs. PCBP2 is part of the ribonucleosome complex supporting various cellular processes by regulating the turnover of target mRNAs. Through these actions it helps control cellular proliferation and immune responses. PCBP2 collaborates with several other proteins influencing cellular adaptation to stress and changes in the environment.

Pathways

PCBP2 plays key roles in the Wnt signaling and Toll-like receptor pathways. In the Wnt signaling pathway its interaction with mRNA targets such as beta-catenin is significant for cellular communication processes. In the Toll-like receptor pathway it works alongside proteins like MyD88 modulating innate immune responses to pathogens. PCBP2's participation in these pathways highlights its versatility and influence on cellular and systemic communication networks.

Researchers have linked PCBP2 to cancer particularly due to its involvement with oncogene regulation. Disruptions in the expression of PCBP2 can affect cell cycle control contributing to tumorigenesis. PCBP2 also connects with amyotrophic lateral sclerosis (ALS) through its interaction with proteins like TDP-43 which are critical in neurodegenerative disease pathways. Understanding PCBP2's function and its connections to these conditions remains important for developing therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Single-stranded nucleic acid binding protein that binds preferentially to oligo dC (PubMed : 12414943, PubMed : 7607214). Major cellular poly(rC)-binding protein (PubMed : 12414943). Also binds poly(rU) (PubMed : 12414943). Acts as a negative regulator of antiviral signaling (PubMed : 19881509, PubMed : 35322803). Negatively regulates cellular antiviral responses mediated by MAVS signaling (PubMed : 19881509). It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitination and degradation (PubMed : 19881509). Negativeley regulates the cGAS-STING pathway via interaction with CGAS, preventing the formation of liquid-like droplets in which CGAS is activated (PubMed : 35322803). Together with PCBP1, required for erythropoiesis, possibly by regulating mRNA splicing (By similarity).. (Microbial infection) In case of infection by poliovirus, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation (PubMed : 12414943, PubMed : 24371074). Also plays a role in initiation of viral RNA replication in concert with the viral protein 3CD (PubMed : 12414943).
See full target information PCBP2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 9:e2204046 PubMed36004483

2022

A Novel Long Non-Coding RNA lnc030 Maintains Breast Cancer Stem Cell Stemness by Stabilizing SQLE mRNA and Increasing Cholesterol Synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yilu Qin,Yixuan Hou,Shuiqing Liu,Pengpeng Zhu,Xueying Wan,Maojia Zhao,Meixi Peng,Huan Zeng,Qiao Li,Ting Jin,Xiaojiang Cui,Manran Liu

Experimental and therapeutic medicine 24:638 PubMed36160905

2022

HuR affects chemoresistance of small cell lung cancer by regulating FGFRL1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Xun-Huang Duan,Rui Chen,Dao-Sheng Li,Ai-Hua Luo,Lin-Lang Guo

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 8:2002232 PubMed33511005

2021

A Novel Long Non-Coding RNA lnc030 Maintains Breast Cancer Stem Cell Stemness by Stabilizing SQLE mRNA and Increasing Cholesterol Synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yilu Qin,Yixuan Hou,Shuiqing Liu,Pengpeng Zhu,Xueying Wan,Maojia Zhao,Meixi Peng,Huan Zeng,Qiao Li,Ting Jin,Xiaojiang Cui,Manran Liu
View all publications
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