AB154252

Anti-PCBP3 antibody

Name: Anti-PCBP3 antibody (ab154252) | Abcam
Brand: Abcam Limited
SKU: AB154252

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(3 Publications )

Rabbit Polyclonal PCBP3 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PCBP3 aa 1-250.

View Alternative Names

PCBP3-OT1, PCBP3OT, PCBP3, Poly(rC)-binding protein 3, Alpha-CP3, PCBP3-overlapping transcript, PCBP3-overlapping transcript 1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-PCBP3 antibody (AB154252)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PCBP3 antibody (AB154252)

Immunofluorescent analysis of methanol-fixed MCF7 cells labeling PCBP3 with ab154252 at 1/500 dilution. The image in the lower panel was co-stained with Hoechst 33342.

Unknown

Western blot - Anti-PCBP3 antibody (AB154252)

12% SDS PAGE

All lanes:

Western blot - Anti-PCBP3 antibody (ab154252) at 1/1000 dilution

All lanes:

IMR32 whole cell lysate at 30 µg

Predicted band size: 39 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human PCBP3 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P57721

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCBP3 also known as Poly(rC)-binding protein 3 plays an important role in RNA binding and regulation. This protein has a molecular mass of approximately 38 kDa. PCBP3 gets expressed in many tissues including liver heart and brain suggesting a widespread functional importance. PCBP3 belongs to a family of proteins that includes PCBP1 and PCBP2 all sharing a conserved hnRNP K homology domain involved in nucleic acid binding.

Biological function summary

Poly(rC)-binding proteins like PCBP3 are involved in a variety of cellular processes including mRNA stabilization and translational control. PCBP3 forms part of ribonucleoprotein complexes influencing RNA metabolism and gene expression regulation. Through these interactions PCBP3 contributes to controlling various physiological processes making it a focus of interest in cellular biology research.

Pathways

This protein interacts within important signaling pathways such as those controlling iron homeostasis and RNA processing. PCBP3 connects with proteins like PCBP1 and PCBP2 in pathways modulating iron metabolism influencing both cellular and systemic iron regulation. As part of these pathways PCBP3 maintains functional integrity of cell processes reliant on iron availability and gene expression.

Poly(rC)-binding protein 3 associations include cancer and neurodegenerative diseases. Alterations in PCBP3 expression or function may link to cancer through dysregulated RNA metabolism and cell cycle control. The protein's involvement in iron metabolism also ties it to neurodegenerative diseases where improper iron homeostasis can result in neuronal damage. In these contexts PCBP3 might interact with other RNA binding proteins and iron regulatory proteins highlighting its clinical relevance in disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Single-stranded nucleic acid binding protein that binds preferentially to oligo dC.

See full target information PCBP3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Biomedical reports 18:22 PubMed36846616

2023

Potential prognostic and predictive value of UBE2N, IMPDH1, DYNC1LI1 and HRASLS2 in colorectal cancer stool specimens.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Nung Chen,Cheng-Yen Shih,Shu-Lin Guo,Chih-Yi Liu,Ming-Hung Shen,Shih-Chang Chang,Wei-Chi Ku,Chi-Cheng Huang,Chi-Jung Huang

Frontiers in pharmacology 12:744826 PubMed34603058

2021

Anlotinib Inhibits PFKFB3-Driven Glycolysis in Myofibroblasts to Reverse Pulmonary Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Weimou Chen,Jinming Zhang,Wenshan Zhong,Yuanyuan Liu,Ye Lu,Zhaojin Zeng,Haohua Huang,Xuan Wan,Xiaojing Meng,Fei Zou,Shaoxi Cai,Hangming Dong

Cell death & disease 12:42 PubMed33414446

2021

TRIB2 modulates proteasome function to reduce ubiquitin stability and protect liver cancer cells against oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Susu Guo,Yuxin Chen,Yueyue Yang,Xiao Zhang,Lifang Ma,Xiangfei Xue,Yongxia Qiao,Jiayi Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PCBP3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-PCBP3 antibody (AB154252)

Western blot - Anti-PCBP3 antibody (AB154252)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise