AB187686

Anti-PCCA antibody - C-terminal

Name: Anti-PCCA antibody - C-terminal (ab187686) | Abcam
Brand: Abcam Limited
SKU: AB187686
Price: 525 USD
Availability: InStock

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(8 Publications)

Rabbit Polyclonal PCCA antibody. C-terminal. Suitable for WB, IHC-Fr and reacts with Human, Mouse samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human PCCA aa 650 to C-terminus.

View Alternative Names

PCCase subunit alpha, Propanoyl-CoA:carbon dioxide ligase subunit alpha, PCCA

3 Images

Immunohistochemistry (Frozen sections) - Anti-PCCA antibody - C-terminal (AB187686)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-PCCA antibody - C-terminal (AB187686)

Immunohistochemistry of human lung cancer tissue staining PCCA with an187686 at 1 : 1000. Detected using DAB.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-PCCA antibody - C-terminal (AB187686)

Immunohistochemistry of human ovarian carcinoma tissue staining PCCA with an187686 at 1 : 1000. Detected using DAB.

Supplier Data

Western blot - Anti-PCCA antibody - C-terminal (AB187686)

All lanes:

Western blot - Anti-PCCA antibody - C-terminal (ab187686) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK1 whole cell lysate at 50 µg

Lane 5:

NIH 3T3 whole cell lysate at 50 µg

Predicted band size: 80 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-Fr

applications

Immunogen

Synthetic Peptide within Human PCCA aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P05165

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500 - 1/2000", "IHCFr-species-notes": "Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab187686 was affinity purified using an epitope specific to PCCA immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCCA also known as propionyl-CoA carboxylase alpha chain is an essential enzyme component in humans. It has a molecular mass of approximately 81.4 kDa and is primarily expressed in the liver and kidney. PCCA forms a biotin-dependent enzyme that catalyzes the carboxylation of propionyl-CoA to form D-methylmalonyl-CoA. This action is an important step in the mitochondrial beta-oxidation of odd-chain fatty acids and some amino acids.

Biological function summary

PCCA is a part of the propionyl-CoA carboxylase complex which functions as a heterododecameric complex involving both alpha and beta subunits. This enzyme plays an important role in energy metabolism facilitating the conversion of propionyl-CoA into more useful forms for cellular processes. The proper functioning of this complex ensures the breakdown and assimilation of important metabolites aiding in the elimination of toxic intermediates produced during the metabolism of branched-chain amino acids as well as odd-chain fatty acids.

Pathways

The catabolism facilitated by PCCA involves the metabolic pathways of branched-chain amino acids and odd-numbered fatty acids. It fits within the larger methylmalonyl-CoA mutase pathway where it transforms its substrate into an immediate precursor for succinyl-CoA production. The activity in this pathway links PCCA to other proteins such as methylmalonyl-CoA mutase which subsequently acts to convert methylmalonyl-CoA to succinyl-CoA a critical step for entry into the tricarboxylic acid (TCA) cycle.

PCCA defects are linked to propionic acidemia a serious metabolic disorder characterized by the accumulation of propionic acid. This disease results from genetic mutations affecting the normal function of the PCCA protein leading to toxic buildup of metabolites in blood and tissues. The disorder can cause severe metabolic acidosis and can impact neurological development. Additionally PCCA interacts with various cellular proteins such as methylmalonyl-CoA mutase which if disrupted also associates with metabolic disorders exemplifying the interdependent relationship between components in metabolic pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites (PubMed : 6765947, PubMed : 8434582). Propionyl-CoA carboxylase catalyzes the carboxylation of propionyl-CoA/propanoyl-CoA to D-methylmalonyl-CoA/(S)-methylmalonyl-CoA (PubMed : 10101253, PubMed : 6765947, PubMed : 8434582). Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then transfers the carboxyl group from carboxylated biotin to propionyl-CoA (By similarity). Propionyl-CoA carboxylase also significantly acts on butyryl-CoA/butanoyl-CoA, which is converted to ethylmalonyl-CoA/(2S)-ethylmalonyl-CoA at a much lower rate (PubMed : 6765947). Other alternative minor substrates include (2E)-butenoyl-CoA/crotonoyl-CoA (By similarity).

See full target information PCCA

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 300:107662 PubMed39128713

2024

Differential utilization of vitamin B-dependent and independent pathways for propionate metabolism across human cells.

Applications

Unspecified application

Species

Unspecified reactive species

Harsha Gouda,Yuanyuan Ji,Sneha Rath,David Watkins,David Rosenblatt,Vamsi Mootha,Jace W Jones,Ruma Banerjee

EMBO molecular medicine 15:e17836 PubMed37766669

2023

Propionate reinforces epithelial identity and reduces aggressiveness of lung carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Vignesh Ramesh,Paradesi Naidu Gollavilli,Luisa Pinna,Mohammad Aarif Siddiqui,Adriana Martinez Turtos,Francesca Napoli,Yasmin Antonelli,Aldo Leal-Egaña,Jesper Foged Havelund,Simon Toftholm Jakobsen,Elisa Le Boiteux,Marco Volante,Nils Joakim Faergeman,Ole N Jensen,Rasmus Siersbaek,Kumar Somyajit,Paolo Ceppi

Molecular therapy. Methods & clinical development 30:181-190 PubMed37746248

2023

Systemic gene therapy using an AAV44.9 vector rescues a neonatal lethal mouse model of propionic acidemia.

Applications

Unspecified application

Species

Unspecified reactive species

Randy J Chandler,Giovanni Di Pasquale,Eun-Young Choi,David Chang,Stephanie N Smith,Jennifer L Sloan,Victoria Hoffmann,Lina Li,John A Chiorini,Charles P Venditti

Proceedings of the National Academy of Sciences of the United States of America 119:e2203628119 PubMed36201541

2022

Succinyl-CoA-based energy metabolism dysfunction in chronic heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Shingo Takada,Satoshi Maekawa,Takaaki Furihata,Naoya Kakutani,Daiki Setoyama,Koji Ueda,Hideo Nambu,Hikaru Hagiwara,Haruka Handa,Yoshizuki Fumoto,Soichiro Hata,Tomoka Masunaga,Arata Fukushima,Takashi Yokota,Dongchon Kang,Shintaro Kinugawa,Hisataka Sabe

International journal of molecular sciences 23: PubMed35562866

2022

Application of a Fluorescence Anisotropy-Based Assay to Quantify Transglutaminase 2 Activity in Cell Lysates.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra Hauser,Paul Sommerfeld,Johanna Wodtke,Christoph Hauser,Paul Schlitterlau,Jens Pietzsch,Reik Löser,Markus Pietsch,Robert Wodtke

Nature metabolism 4:435-443 PubMed35361954

2022

Altered propionate metabolism contributes to tumour progression and aggressiveness.

Applications

Unspecified application

Species

Unspecified reactive species

Ana P Gomes,Didem Ilter,Vivien Low,Stanislav Drapela,Tanya Schild,Edouard Mullarky,Julie Han,Ilaria Elia,Dorien Broekaert,Adam Rosenzweig,Michal Nagiec,Joana B Nunes,Bethany E Schaffer,Anders P Mutvei,John M Asara,Lewis C Cantley,Sarah-Maria Fendt,John Blenis

Nature communications 11:3501 PubMed32647108

2020

Antisense oligonucleotide modulation of non-productive alternative splicing upregulates gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Kian Huat Lim,Zhou Han,Hyun Yong Jeon,Jacob Kach,Enxuan Jing,Sebastien Weyn-Vanhentenryck,Mikaela Downs,Anna Corrionero,Raymond Oh,Juergen Scharner,Aditya Venkatesh,Sophina Ji,Gene Liau,Barry Ticho,Huw Nash,Isabel Aznarez

Biochemical and biophysical research communication 503:2619-2624 PubMed30100068

2018

Caspase-1 regulates cellular trafficking via cleavage of the Rab7 adaptor protein RILP.

Applications

Unspecified application

Species

Unspecified reactive species

Abby Adams,Steven A Weinman,Ann L Wozniak

View all publications

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Product promise

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Anti-PCCA antibody - C-terminal

Immunohistochemistry (Frozen sections) - Anti-PCCA antibody - C-terminal (AB187686)

Immunohistochemistry (Frozen sections) - Anti-PCCA antibody - C-terminal (AB187686)

Western blot - Anti-PCCA antibody - C-terminal (AB187686)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

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