AB96729

Anti-PCCB antibody

Name: Anti-PCCB antibody (ab96729) | Abcam
Brand: Abcam Limited
SKU: AB96729
Price: 535 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal PCCB antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PCCB aa 150-450.

View Alternative Names

PCCase subunit beta, Propanoyl-CoA:carbon dioxide ligase subunit beta, PCCB

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCCB antibody (AB96729)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCCB antibody (AB96729)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labelling PCCB protein at mitochondria with ab96729 at a 1/500 dilution.
Antigen retrieval : EDTA based, pH 8.0 buffer, 15min

Unknown

Western blot - Anti-PCCB antibody (AB96729)

7.5% SDS PAGE

All lanes:

Western blot - Anti-PCCB antibody (ab96729) at 1/1000 dilution

All lanes:

HepG2 whole cell lysate at 30 µg

Predicted band size: 58 kDa

false

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCCB antibody (AB96729)

Immunohistochemical analysis of paraffin-embedded Lung Ad Cancer PC14, using ab96729 at 1 : 100 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PCCB aa 150-450. The exact immunogen used to generate this antibody is proprietary information.

P05166

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCCB also known as Propionyl-CoA Carboxylase β subunit is an enzyme component that plays an important role in metabolizing certain amino acids and odd-chain fatty acids. This protein partners with the alpha subunit to form a dimeric enzyme with a total mass of approximately 750 kDa. PCCB is mainly expressed in the liver but its expression also occurs in other tissues such as the kidney and heart where energy and metabolism play key roles.

Biological function summary

Propionyl-CoA Carboxylase β subunit takes part in the process of carboxylation of propionyl-CoA to methylmalonyl-CoA an essential step in catabolism. This subunit contributes to the function of the complex necessary for efficient energy production and proper detoxification of specific intermediary products. In this enzymatic action it works jointly with the alpha subunit that harbors the biotin-binding sites important for transferring carbon dioxide.

Pathways

PCCB is an important component in the propionic acid pathway and is critical for maintaining the balance in mitochondrial fatty acid synthesis. Its function plays a central role in the catabolism of certain amino acids including valine isoleucine and methionine as well as odd-chain fatty acids. Propionyl-CoA Carboxylase β subunit’s action works closely with methylmalonyl-CoA mutase assisting in metabolic conversion that takes place after the initial carboxylation step.

Deficiencies or mutations in PCCB are linked to propionic acidemia a metabolic disorder that interrupts normal propionic acid breakdown leading to an accumulation that is toxic to the body. This deficiency relates it to methylmalonic acidemia if methylmalonyl-CoA mutase is affected often causing similar clinical symptoms such as developmental delays vomiting and metabolic crises. Understanding the function and pathways of PCCB helps to develop targeted therapies that address its metabolic roles and its involvement in these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites (PubMed : 15890657, PubMed : 6765947). Propionyl-CoA carboxylase catalyzes the carboxylation of propionyl-CoA/propanoyl-CoA to D-methylmalonyl-CoA/(S)-methylmalonyl-CoA (PubMed : 15890657, PubMed : 6765947). Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then transfers the carboxyl group from carboxylated biotin to propionyl-CoA (By similarity). Propionyl-CoA carboxylase also significantly acts on butyryl-CoA/butanoyl-CoA, which is converted to ethylmalonyl-CoA/(2S)-ethylmalonyl-CoA at a much lower rate (PubMed : 6765947). Other alternative minor substrates include (2E)-butenoyl-CoA/crotonoyl-CoA (By similarity).

See full target information PCCB

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell biology international 47:1799-1812 PubMed37434531

2023

RGMb impacts partial epithelial-mesenchymal transition and BMP2-Induced ID mRNA expression independent of PD-L2 in nonsmall cell lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sofie Rahbek Dorset,Tina Fuglsang Daugaard,Trine Vilsbøll Larsen,Anders Lade Nielsen

Orphanet journal of rare diseases 17:62 PubMed35189944

2022

Therapeutic potential of living donor liver transplantation from heterozygous carrier donors in children with propionic acidemia.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Gui Zeng,Guang-Peng Zhou,Lin Wei,Wei Qu,Ying Liu,Yu-Le Tan,Jun Wang,Li-Ying Sun,Zhi-Jun Zhu

Thyroid : official journal of the American Thyroid Association 29:845-857 PubMed30990120

2019

Reprogramming of Energy Metabolism: Increased Expression and Roles of Pyruvate Carboxylase in Papillary Thyroid Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Aurélie Strickaert,Cyril Corbet,Selim-Alex Spinette,Ligia Craciun,Geneviève Dom,Guy Andry,Denis Larsimont,Ruddy Wattiez,Jacques E Dumont,Olivier Feron,Carine Maenhaut

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PCCB antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCCB antibody (AB96729)

Western blot - Anti-PCCB antibody (AB96729)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCCB antibody (AB96729)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise