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AB203520

Anti-PCDH11X antibody [EPR16916]

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(1 Publication)

Rabbit Recombinant Monoclonal PCDH11X antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 1 publication.

View Alternative Names

KIAA1326, PCDH11, PCDHX, PCDH11X, Protocadherin-11 X-linked, Protocadherin-11, Protocadherin on the X chromosome, Protocadherin-S, PCDH-X

3 Images
Western blot - Anti-PCDH11X antibody [EPR16916] (AB203520)
  • WB

Supplier Data

Western blot - Anti-PCDH11X antibody [EPR16916] (AB203520)

Blocking and diluting buffer :

5% NFDM/TBST

All lanes:

Western blot - Anti-PCDH11X antibody [EPR16916] (ab203520) at 1/1000 dilution

Lane 1:

Human Hippocampus lysate at 20 µg

Lane 2:

Human heart lysate at 20 µg

Lane 3:

Human kidney lysate at 20 µg

Lane 4:

Human spleen lysate at 20 µg

Lane 5:

Mouse hippocampus lysate at 20 µg

Lane 6:

Mouse cerebellum lysate at 20 µg

Lane 7:

Mouse heart lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 148 kDa

Observed band size: 148 kDa

false

Exposure time: 180s

Western blot - Anti-PCDH11X antibody [EPR16916] (AB203520)
  • WB

Supplier Data

Western blot - Anti-PCDH11X antibody [EPR16916] (AB203520)

Blocking/Dilution buffer : 5% NFDM/TBST.

PCDH11X protein is expressed strongly in fetal brain and brain (cortex, amygdala, thalamus, substantia nigra, hippocampus, caudate nucleus and corpus callosum).

All lanes:

Western blot - Anti-PCDH11X antibody [EPR16916] (ab203520) at 1/1000 dilution

Lane 1:

Human hippocampus lysate at 10 µg

Lane 2:

Human cerebellum lysate at 10 µg

Lane 3:

Human fetal brain lysate at 10 µg

Lane 4:

Human fetal heart lysate at 10 µg

Lane 5:

Human fetal kidney lysate at 10 µg

Lane 6:

Human fetal spleen lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

false

Western blot - Anti-PCDH11X antibody [EPR16916] (AB203520)
  • WB

Supplier Data

Western blot - Anti-PCDH11X antibody [EPR16916] (AB203520)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PCDH11X antibody [EPR16916] (ab203520) at 1/1000 dilution

Lane 1:

Mouse thalamus lysate at 10 µg

Lane 2:

Mouse hippocampus lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 148 kDa

Observed band size: 148 kDa

false

Exposure time: 3min

  • Carrier free

    Anti-PCDH11X antibody [EPR16916] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16916

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PCDH11X also known as Protocadherin 11 X-linked is a protein encoded by the PCDH11X gene. It weighs around 170 kDa. This protein is expressed mainly in the brain with higher expression in cerebral cortices and hippocampus regions. PCDH11X belongs to the protocadherin family known for their role in cell adhesion. Mechanically it facilitates cell-cell interactions supporting neuronal connectivity and synapse formation.
Biological function summary

PCDH11X plays a critical role in neuronal development and differentiation. It engages in homophilic adhesion allowing neurons to communicate and establish complex networks. PCDH11X functions within synaptic junctions and contributes to maintaining neural circuitry. It often interacts with other protocadherins and is considered part of a larger adhesion protein complex which includes cadherins known to regulate cellular architecture.

Pathways

PCDH11X takes part in neural circuitry development and synaptic plasticity pathways. Notably it participates in the Wnt signaling pathway which is essential for neuronal patterning and connectivity. PCDH11X shares interaction with proteins such as beta-catenin which link cell adhesion molecules to the actin cytoskeleton further influencing signaling pathways related to neural morphogenesis.

PCDH11X shows relevance to neurodevelopmental disorders and mental health conditions like schizophrenia. Researchers notice alterations in PCDH11X expression linked to these conditions suggesting its importance in neuropsychiatric health. Additionally it is connected to the N-cadherin protein which also associates with cognitive disorders emphasizing its role in maintaining proper neural function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Potential calcium-dependent cell-adhesion protein.
See full target information PCDH11X
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer biology & therapy 24:2225884 PubMed37382415

2023

BTF3 promotes proliferation and glycolysis in hepatocellular carcinoma by regulating GLUT1.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Wang,Jianmin Sun,Chengming Sun,Haoran Zhao,YuBao Zhang,Jing Chen
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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